Source 1primary paper2022
Toolkit/Bessel beam stimulation source integrated into LLSM
Bessel beam stimulation source integrated into LLSM
Also known as: Bessel beam
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The Bessel beam stimulation source integrated into lattice light-sheet microscopy (LLSM) is an optical assay configuration that adds Bessel-beam-based optogenetic stimulation to LLSM without changing the microscope optical configuration. It enables three-dimensional photoactivation with improved spatiotemporal control and has been used to manipulate subcellular membrane ruffling and guide cell migration.
Usefulness & Problems
Why this is useful
This configuration is useful for optogenetic experiments that require spatially confined activation within cells while simultaneously performing volumetric imaging. Reported applications include subcellular activation of membrane ruffling at different intracellular locations and long-term guidance of cell migration during repeated volume acquisition.
Source:
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Source:
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
Problem solved
It addresses the problem of achieving precise optogenetic photoactivation inside the 3D imaging context of LLSM with high spatiotemporal control. The reported implementation specifically supports subcellularly resolved stimulation during live-cell observation of migration-related behaviors.
Source:
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Source:
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
Problem links
Need precise spatiotemporal control with light input
DerivedThe Bessel beam stimulation source integrated into lattice light-sheet microscopy (LLSM) is an optical assay configuration that adds Bessel-beam-based optogenetic stimulation to LLSM without changing the microscope optical configuration. It enables three-dimensional photoactivation with improved spatiotemporal control and has been used to manipulate subcellular membrane ruffling and guide cell migration.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The stimulation source was integrated into LLSM as a Bessel beam without changing the optical configuration. Beyond this integration detail, the evidence does not specify construct design, illumination wavelength, hardware components, or sample preparation requirements.
The supplied evidence is limited to a single 2022 source and a small set of application outcomes. No quantitative optical parameters, wavelength requirements, compatible optogenetic actuators, or cross-platform validation details are provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
guided cell migration duration 6 himaging volumes collected 463 imaging volumes
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
Lattice lightsheet microscopy was modified to enable three-dimensional optogenetic activation with subcellular resolution for manipulation of cellular behavior.
Lattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation.
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
required energy power for optogenetic reactions 1 nWrequired power density for optogenetic reactions 24 mW/cm 2
Approval Evidence
1 source4 linked approval claimsfirst-pass slug bessel-beam-stimulation-source-integrated-into-llsm
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
Source:
application resultsupports
Membrane ruffling could be activated at different locations within a cell with subcellular resolution.
membrane ruffling can be activated at different locations within a cell with subcellular resolution
Source:
application resultsupports
Optogenetic stimulation enabled guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage.
We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
Source:
implementationsupports
A Bessel beam stimulation source was integrated into LLSM without changing the optical configuration, improving spatiotemporal control of photoactivation.
As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation.
Source:
performancesupports
The energy power required for optogenetic reactions was lower than 1 nW or 24 mW/cm2.
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
Source:
Comparisons
Source-backed strengths
The reported system achieved activation of membrane ruffling at different locations within a cell with subcellular resolution. It also supported guided cell migration for up to 6 hours while collecting 463 imaging volumes without noticeable cell damage, indicating compatibility with extended live-cell imaging.
Source:
We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm 2 )
Compared with Langendorff perfused heart electrical recordings
Bessel beam stimulation source integrated into LLSM and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with lattice lightsheet microscopy
Bessel beam stimulation source integrated into LLSM and lattice lightsheet microscopy address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: optogenetic photoactivation; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with native green gel system
Bessel beam stimulation source integrated into LLSM and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.