Toolkit/bisulfite pyrosequencing

bisulfite pyrosequencing

Assay Method·Research·Since 2014

Also known as: bisulfite pyrosequencing technology

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Bisulfite pyrosequencing is an assay method used to quantify promoter CpG DNA methylation after bisulfite treatment. In the cited study, it was used to measure methylation in the CAMK1D, CRY2, and CALM2 promoter regions in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Usefulness & Problems

Why this is useful

This method is useful for locus-specific measurement of promoter CpG methylation in biological samples. The supplied evidence supports its use for comparing methylation levels between disease and control cohorts in peripheral blood.

Problem solved

It addresses the need to measure promoter DNA methylation levels at specific genes of interest. In the cited application, it enabled assessment of methylation at CAMK1D, CRY2, and CALM2 promoters in type 2 diabetes versus matched healthy controls.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Target processes

No target processes tagged yet.

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor

The method, as evidenced here, involves bisulfite pyrosequencing technology applied to promoter regions of CAMK1D, CRY2, and CALM2. The reported sample source was peripheral blood from patients with type 2 diabetes and matched healthy controls; no further protocol details are provided.

The supplied evidence is limited to a single study and a single application context. No performance metrics, resolution details, throughput characteristics, or independent validation data are provided in the evidence.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48
Claim 2measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48
Claim 3measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48
Claim 4measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48
Claim 5measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48
Claim 6measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48
Claim 7measurement methodsupports2014Source 1needs review

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.
healthy controls 48patients with T2D 48

Approval Evidence

1 source1 linked approval claimfirst-pass slug bisulfite-pyrosequencing
Using bisulfite pyrosequencing technology

Source:

measurement methodsupports

Promoter CpG DNA methylation levels of CAMK1D, CRY2, and CALM2 were measured using bisulfite pyrosequencing in peripheral blood from patients with type 2 diabetes and matched healthy controls.

Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls.

Source:

Comparisons

Source-backed strengths

The evidence shows that the method can be applied to promoter CpG methylation analysis in human peripheral blood samples. It was used on defined target genes in a case-control study context, indicating suitability for targeted methylation quantification.

Compared with fluorescence line narrowing

bisulfite pyrosequencing and fluorescence line narrowing address a similar problem space.

Shared frame: same top-level item type

Compared with Langendorff perfused heart electrical recordings

bisulfite pyrosequencing and Langendorff perfused heart electrical recordings address a similar problem space.

Shared frame: same top-level item type

Strengths here: looks easier to implement in practice.

Compared with native green gel system

bisulfite pyrosequencing and native green gel system address a similar problem space.

Shared frame: same top-level item type

Strengths here: looks easier to implement in practice.

Ranked Citations

  1. 1.
    StructuralSource 1Experimental and Therapeutic Medicine2014Claim 1Claim 2Claim 3

    Extracted from this source document.