Source 1primary paper2024Angewandte Chemie
Toolkit/Boolean logic gate
Boolean logic gate
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The Boolean logic gate is a construct pattern incorporated into a photoactivatable CRISPR/Cas9-inducible DNAzyme probe. In the reported system, it contributed to superior spatiotemporal control for dynamic imaging of nuclear Zn2+ in HeLa cells and mice.
Usefulness & Problems
Why this is useful
This construct pattern is useful for tightening conditional control over probe activation in a light-responsive CRISPR-DNAzyme imaging system. The reported implementation enabled in situ imaging of nuclear Zn2+ in living cells and improved spatiotemporal control during dynamic monitoring.
Source:
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
Problem solved
It addresses the problem of achieving precise spatiotemporal activation of a DNAzyme-based imaging probe in the nucleus. In the cited design, Boolean logic gating was combined with photoactivation and CRISPR/Cas9-triggered probe conversion to reduce premature activity and support controlled nuclear Zn2+ imaging.
Source:
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
Problem links
Need controllable genome or transcript editing
DerivedThe Boolean logic gate is a construct pattern incorporated into a photoactivatable CRISPR/Cas9-inducible DNAzyme probe. In the reported system, it contributed to superior spatiotemporal control for dynamic imaging of nuclear Zn2+ in HeLa cells and mice.
Need precise spatiotemporal control with light input
DerivedThe Boolean logic gate is a construct pattern incorporated into a photoactivatable CRISPR/Cas9-inducible DNAzyme probe. In the reported system, it contributed to superior spatiotemporal control for dynamic imaging of nuclear Zn2+ in HeLa cells and mice.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
boolean logic gatingconditional dnazyme activationcrispr/cas9-mediated cleavagephotoactivationTechniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The reported probe is a three-stranded DNAzyme construct containing a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage generates the catalytic DNAzyme structure in the nucleus. The system was integrated with a photoactivation strategy and applied in HeLa cells and mice, but the supplied evidence does not detail the photochemical cage, illumination wavelength, or delivery format.
The available evidence describes Boolean logic gate integration at a high level but does not specify the exact logical architecture, inputs, or truth table. Quantitative performance metrics, generalizability beyond nuclear Zn2+ imaging, and independent replication are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
The CRISPR/Cas9-inducible DNAzyme design enables in situ imaging of nuclear Zn2+ in living cells.
With this design, the CRISPR/Cas9‐inducible imaging of nuclear Zn 2+ is demonstrated in living cells.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
The three-stranded DNAzyme probe contains a 20-bp CRISPR/Cas9 recognition site that blocks DNAzyme activity until Cas9/sgRNA cleavage forms the catalytic DNAzyme structure in the nucleus.
We developed a three‐stranded DNAzyme probe (TSDP) that contained a 20‐base‐pair (20‐bp) recognition site of a CRISPR/Cas9, which blocks the DNAzyme activity. When Cas9, with its specialized nuclear localization function, forms an active complex with sgRNA within the cell nucleus, it cleaves the TSDP at the recognition site, resulting in the in situ formation of catalytic DNAzyme structure.
recognition site length 20 bp
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
This conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and provides new analytical methods for nuclear metal-associated biology.
Collectively, this conceptual design expands the DNAzyme toolbox for visualizing nuclear metal ions and thus provides new analytical methods for nuclear metal‐associated biology.
Approval Evidence
1 source1 linked approval claimfirst-pass slug boolean-logic-gate
the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate
Source:
comparative performancesupports
Integrating the CRISPR-DNAzyme system with photoactivation strategy and Boolean logic gate provided superior spatiotemporal control imaging for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice.
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Source:
Comparisons
Source-backed strengths
The integrated system was reported to provide superior spatiotemporal control imaging when Boolean logic gating was combined with photoactivation and the CRISPR-DNAzyme design. The platform was demonstrated for dynamic monitoring of nuclear Zn2+ in HeLa cells and mice, indicating validation in both cultured cells and an in vivo context.
Source:
Moreover, the superiority of CRISPR‐DNAzyme for spatiotemporal control imaging was demonstrated by integrating it with photoactivation strategy and Boolean logic gate for dynamic monitoring nuclear Zn 2+ in both HeLa cells and mice.
Compared with retinal prostheses
Boolean logic gate and retinal prostheses address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with three-stranded DNAzyme probe
Boolean logic gate and three-stranded DNAzyme probe address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: boolean logic gating, crispr/cas9-mediated cleavage
Relative tradeoffs: looks easier to implement in practice.
Compared with tube-in-tube structure
Boolean logic gate and tube-in-tube structure address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photoactivation; same primary input modality: light
Ranked Citations
- 1.