Toolkit/Booster

Booster

Construct Pattern·Research·Since 2020

Also known as: Booster backbone, Booster biosensor backbone, Booster biosensors

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Booster is a red-shifted genetically encoded FRET biosensor backbone generated by optimizing the order of fluorescent proteins and modulatory domains within a biosensor architecture. In the reported implementation, a Booster-PKA sensor enabled kinase activity readout in a spectral window compatible with CFP/YFP-based FRET biosensors and blue light-responsive optogenetic tools.

Usefulness & Problems

Why this is useful

Booster is useful for multiplexed live-cell signaling measurements when standard CFP/YFP FRET sensors create spectral conflicts. The reported PKA implementation could be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities in the same experiment.

Source:

Booster is a red-shifted FRET biosensor backbone created by optimizing fluorescent protein order and modulatory domains. It enables genetically encoded activity sensing with excitation and emission shifted away from standard CFP/YFP pairs.

Source:

building red-shifted genetically encoded FRET biosensors

Source:

multiplexed imaging alongside CFP/YFP-based FRET biosensors

Source:

compatibility with blue light-responsive optogenetic tools

Problem solved

Booster addresses spectral incompatibility between conventional CFP/YFP-based FRET biosensors and experiments that require either multiplexed FRET imaging or blue light-responsive optogenetic tools. Its red-shifted backbone is intended to move excitation and emission away from the CFP/YFP spectral window.

Source:

Booster addresses the spectral incompatibility of standard CFP/YFP FRET biosensors with multiplexed FRET imaging and with blue light-responsive optogenetic tools. The red-shifted design is intended to allow these combinations in the same experiment.

Source:

CFP/YFP-based FRET biosensors preclude use of multiple FRET biosensors within a single cell

Source:

CFP/YFP-based FRET biosensors are incompatible with optogenetic tools that operate at blue light

Problem links

addresses incompatibility between CFP/YFP FRET biosensors and blue-light optogenetic tools

Literature

Booster addresses the difficulty of combining standard CFP/YFP FRET biosensors in one cell and their incompatibility with blue-light optogenetic tools.

Source:

Booster addresses the difficulty of combining standard CFP/YFP FRET biosensors in one cell and their incompatibility with blue-light optogenetic tools.

CFP/YFP-based FRET biosensors are incompatible with optogenetic tools that operate at blue light

Literature

Booster addresses the spectral incompatibility of standard CFP/YFP FRET biosensors with multiplexed FRET imaging and with blue light-responsive optogenetic tools. The red-shifted design is intended to allow these combinations in the same experiment.

Source:

Booster addresses the spectral incompatibility of standard CFP/YFP FRET biosensors with multiplexed FRET imaging and with blue light-responsive optogenetic tools. The red-shifted design is intended to allow these combinations in the same experiment.

CFP/YFP-based FRET biosensors preclude use of multiple FRET biosensors within a single cell

Literature

Booster addresses the spectral incompatibility of standard CFP/YFP FRET biosensors with multiplexed FRET imaging and with blue light-responsive optogenetic tools. The red-shifted design is intended to allow these combinations in the same experiment.

Source:

Booster addresses the spectral incompatibility of standard CFP/YFP FRET biosensors with multiplexed FRET imaging and with blue light-responsive optogenetic tools. The red-shifted design is intended to allow these combinations in the same experiment.

reduces spectral conflict that precludes use of multiple CFP/YFP FRET biosensors within a single cell

Literature

Booster addresses the difficulty of combining standard CFP/YFP FRET biosensors in one cell and their incompatibility with blue-light optogenetic tools.

Source:

Booster addresses the difficulty of combining standard CFP/YFP FRET biosensors in one cell and their incompatibility with blue-light optogenetic tools.

Published Workflows

Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools

2020

Objective: Develop a red-shifted genetically encoded FRET biosensor backbone that avoids the multiplexing and blue-light compatibility limitations of CFP/YFP-based biosensors, then demonstrate its utility in vitro and in vivo.

Why it works: The workflow pairs a favorable red-shifted donor/acceptor set selected by Förster distance calculations with biosensor architecture optimization, then tests whether the resulting design retains biosensor performance while reducing spectral conflicts with other FRET sensors and blue-light optogenetic tools.

Förster resonance energy transfer-based reporting of signaling activityspectral separation to enable multiplexed imaging and optogenetic compatibilityFörster distance-based donor/acceptor selectionoptimization of fluorescent protein orderoptimization of modulatory domain arrangementproof-of-concept application testing

Stages

  1. 1.
    Donor-acceptor pair selection by Förster distance calculation(in_silico_filter)

    This stage identifies a donor/acceptor pair suitable for building a red-shifted FRET biosensor.

    Selection: Favorable donor and acceptor pair chosen by calculating the Förster distance.

  2. 2.
    Biosensor backbone optimization(library_design)

    This stage converts the selected fluorescent protein pair into a working biosensor backbone.

    Selection: Optimization of fluorescent protein order and modulatory domains.

  3. 3.
    Benchmarking with a PKA biosensor implementation(functional_characterization)

    This stage checks whether the red-shifted backbone retains useful biosensor performance after engineering.

    Selection: Comparison of Booster-PKA performance to AKAR3EV.

  4. 4.
    Proof-of-concept compatibility demonstrations(confirmatory_validation)

    This stage confirms that the engineered spectral shift solves the intended compatibility problems in live-cell use cases.

    Selection: Demonstration of simultaneous kinase monitoring and compatibility with a blue light-responsive optogenetic tool.

  5. 5.
    In vivo tissue imaging in transgenic mice(in_vivo_validation)

    This stage validates that the biosensor can function in living tissues in an animal context, extending beyond in vitro demonstrations.

    Selection: Presentation of PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Steps

  1. 1.
    Calculate Förster distance to choose donor and acceptor fluorescent proteins

    Identify a favorable red-shifted donor/acceptor pair for the biosensor.

    Pair selection is performed before biosensor backbone optimization because the fluorescent proteins define the core FRET pair used in the design.

  2. 2.
    Optimize fluorescent protein order and modulatory domains to build the Booster backboneengineered biosensor backbone

    Convert the selected fluorescent protein pair into a functional red-shifted FRET biosensor backbone.

    Architecture optimization follows pair selection because the chosen donor and acceptor must be arranged with modulatory domains to create a working biosensor.

  3. 3.
    Implement the Booster backbone as a PKA biosensor and compare it with AKAR3EVbiosensor under test and benchmark comparator

    Determine whether the engineered red-shifted backbone retains practical biosensor performance.

    Benchmarking occurs after backbone construction to verify that the redesigned sensor remains functionally comparable to an established PKA biosensor before broader application claims.

  4. 4.
    Test simultaneous monitoring with a CFP/YFP-based ERK FRET biosensorbiosensor under application test

    Demonstrate multiplexed kinase activity imaging with a standard CFP/YFP-based FRET biosensor.

    This proof-of-concept follows benchmarking because the authors next test whether the red-shifted design solves the intended multiplexing limitation.

  5. 5.
    Test monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclasebiosensor-actuator compatibility pair

    Demonstrate compatibility of the red-shifted biosensor with a blue light-responsive optogenetic tool.

    After showing multiplexed biosensor compatibility, the authors next test the second intended use case: compatibility with blue light-responsive optogenetic control.

  6. 6.
    Image PKA activity in living tissues of transgenic mice expressing Booster-PKAbiosensor under in vivo validation

    Extend validation from in vitro proof-of-concept experiments to living tissue imaging in an animal model.

    In vivo tissue imaging is presented last as a higher-context validation of versatility after in vitro compatibility demonstrations.

Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools

2020

Objective: Develop a red-shifted genetically encoded FRET biosensor backbone that avoids the multiplexing and blue-light incompatibility limitations of CFP/YFP-based FRET biosensors, and demonstrate its utility with a PKA biosensor in vitro and in vivo.

Why it works: The workflow first addresses spectral design by selecting a favorable donor-acceptor pair and optimizing biosensor architecture, then tests whether the resulting backbone retains sensing performance while enabling multiplexing and blue-light optogenetic compatibility.

Förster resonance energy transferPKA activity sensingoptogenetic cAMP-driven PKA activationFörster distance-based fluorophore pair selectionoptimization of fluorescent protein orderoptimization of modulatory domain ordercomparative biosensor benchmarkingproof-of-concept application testing

Stages

  1. 1.
    Fluorophore pair selection(in_silico_filter)

    This stage identifies a donor-acceptor pair suitable for building red-shifted FRET biosensors.

    Selection: Calculated Förster distance used to choose a favorable donor and acceptor pair.

  2. 2.
    Backbone optimization(library_design)

    This stage converts the selected fluorophore pair into a working biosensor backbone.

    Selection: Optimization of the order of fluorescent proteins and modulatory domains.

  3. 3.
    Comparator performance testing(confirmatory_validation)

    This stage checks whether the red-shifted backbone preserves performance relative to an established CFP/YFP PKA biosensor.

    Selection: Comparison of Booster-PKA performance to AKAR3EV.

  4. 4.
    Multiplexing proof of concept(functional_characterization)

    This stage tests whether the red-shifted design enables simultaneous use with standard CFP/YFP biosensors.

    Selection: Ability to monitor two protein kinase activities simultaneously with Booster-PKA and a CFP/YFP ERK FRET biosensor.

  5. 5.
    Optogenetic compatibility testing(functional_characterization)

    This stage tests whether the red-shifted biosensor can operate with a blue-light optogenetic actuator that would conflict with CFP/YFP biosensors.

    Selection: Ability to monitor PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

  6. 6.
    In vivo tissue demonstration(in_vivo_validation)

    This stage extends validation from in vitro demonstrations to living tissues in transgenic mice.

    Selection: Presentation of PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Steps

  1. 1.
    Calculate Förster distance to choose donor and acceptor pair

    Identify a favorable fluorescent protein pair for a red-shifted FRET biosensor.

    Pair selection is done before backbone optimization because the chosen donor and acceptor define the spectral basis of the biosensor.

  2. 2.
    Optimize fluorescent protein and modulatory domain order to build Boosterengineered biosensor backbone

    Convert the selected fluorophore pair into a functional red-shifted FRET biosensor backbone.

    Architecture optimization follows fluorophore selection because domain order must be tuned around the chosen donor-acceptor pair.

  3. 3.
    Benchmark Booster-PKA against AKAR3EVbiosensor and comparator

    Test whether the red-shifted PKA biosensor preserves performance relative to an established CFP/YFP biosensor.

    Comparator benchmarking is performed after backbone construction to confirm that solving spectral compatibility did not compromise sensing performance.

  4. 4.
    Test simultaneous kinase monitoring with Booster-PKA and a CFP/YFP ERK biosensorbiosensor under application test

    Demonstrate multiplexed monitoring of two kinase activities in the same setting.

    This application test follows comparator benchmarking because multiplexing is a key intended advantage once baseline performance is established.

  5. 5.
    Monitor PKA activation driven by Beggiatoa photoactivated adenylyl cyclasebiosensor and optogenetic actuator

    Demonstrate compatibility of the red-shifted biosensor with a blue-light optogenetic cAMP generator.

    This test follows multiplexing proof of concept because blue-light compatibility is another central design goal enabled by spectral red-shifting.

  6. 6.
    Present PKA activity in living tissues of transgenic mice expressing Booster-PKAbiosensor under in vivo validation

    Demonstrate that the biosensor can report PKA activity in living mouse tissues.

    In vivo demonstration is placed after in vitro proof-of-concept tests as a higher-fidelity validation of practical imaging utility.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A reusable architecture pattern for arranging parts into an engineered system.

Target processes

No target processes tagged yet.

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor

The reported backbone uses mKOκ as the donor fluorescent protein and mKate2 as the acceptor fluorescent protein. It is implemented as a biosensor architecture that requires appropriate modulatory domains for the signaling activity being monitored, with the backbone created by optimizing the order of these domains and the fluorescent proteins.

The supplied evidence supports a PKA implementation and proof-of-concept multiplexing use, but it does not establish broad generalization across many signaling targets. Independent replication is not provided in the supplied evidence, and quantitative performance details beyond comparability to AKAR3EV are not available here.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Source 1primary paper2020ACS Sensors
1 ranked citation104 ranked claimsCitation 1Claim 1Claim 2Claim 3

Ranked Claims

Claim 1application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 2application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 3application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 4application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 5application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 6application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 7application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 8application capabilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor activities of two protein kinases.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 9application capabilitysupports2020Source 1needs review

Booster-PKA enabled simultaneous monitoring of two protein kinase activities together with a CFP/YFP-based ERK FRET biosensor.

For the proof of concept, we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP.
Claim 10comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 11comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 12comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 13comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 14comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 15comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 16comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 17comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 18comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 19comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 20comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 21comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 22comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 23comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 24comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV
Claim 25comparative performancesupports2020Source 1needs review

Booster-PKA performance was comparable to that of AKAR3EV.

The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV, a previously developed FRET biosensor comprising CFP and YFP.
Claim 26compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 27compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 28compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 29compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 30compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 31compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 32compatibilitysupports2020Source 1needs review

Booster-PKA can be used simultaneously with a CFP/YFP-based ERK FRET biosensor to monitor two protein kinase activities.

we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP
Claim 33compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 34compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 35compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 36compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 37compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 38compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 39compatibilitysupports2020Source 1needs review

Booster-PKA enabled monitoring of PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP
Claim 40compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 41compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 42compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 43compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 44compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 45compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 46compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 47compatibilitysupports2020Source 1needs review

Booster-PKA is compatible with blue-light optogenetic activation of cAMP signaling using Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 48compatibilitysupports2020Source 1needs review

Booster-PKA was used to monitor PKA activation driven by Beggiatoa photoactivated adenylyl cyclase.

Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP.
Claim 49design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 50design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 51design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 52design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 53design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 54design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 55design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 56design rationalesupports2020Source 1needs review

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.
Claim 57engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 58engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 59engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 60engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 61engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 62engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 63engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 64engineering outcomesupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".
Claim 65engineering outcomesupports2020Source 1needs review

The authors developed red-shifted FRET biosensors and a biosensor backbone named Booster by choosing mKOκ and mKate2 and optimizing fluorescent protein order and modulatory domains.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 66engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 67engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 68engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 69engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 70engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 71engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 72engineering resultsupports2020Source 1needs review

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".
Claim 73in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 74in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 75in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 76in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 77in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 78in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 79in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 80in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 81in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 82in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 83in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 84in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 85in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 86in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 87in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 88in vivo applicationsupports2020Source 1needs review

Booster-PKA was used to present PKA activity in living tissues of transgenic mice expressing Booster-PKA.

Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA.
Claim 89overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 90overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 91overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 92overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 93overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 94overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 95overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 96overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 97overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 98overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 99overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 100overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 101overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 102overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 103overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
Claim 104overall conclusionsupports2020Source 1needs review

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.

Approval Evidence

1 source7 linked approval claimsfirst-pass slug booster
By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".

Source:

design rationalesupports

The Booster design selected mKOκ and mKate2 as the donor and acceptor pair based on calculated Förster distance.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance.

Source:

engineering outcomesupports

Booster is a red-shifted genetically encoded FRET biosensor backbone developed to overcome limitations of CFP/YFP-based FRET biosensors for multiplexing and blue-light optogenetic compatibility.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths... we developed a FRET biosensor backbone named "Booster".

Source:

engineering outcomesupports

The authors developed red-shifted FRET biosensors and a biosensor backbone named Booster by choosing mKOκ and mKate2 and optimizing fluorescent protein order and modulatory domains.

We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".

Source:

engineering resultsupports

Booster is a red-shifted genetically encoded FRET biosensor backbone developed by optimizing fluorescent protein order and modulatory domains.

To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster".

Source:

overall conclusionsupports

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.

Source:

overall conclusionsupports

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.

Source:

overall conclusionsupports

Booster biosensors are effective and versatile imaging tools in vitro and in vivo.

Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.

Source:

Comparisons

Source-stated alternatives

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Source-backed strengths

The backbone was specifically engineered by optimizing fluorescent protein and modulatory domain order, yielding a red-shifted FRET design. In the reported PKA sensor, performance was comparable to AKAR3EV, and the construct supported simultaneous monitoring with a CFP/YFP-based ERK FRET biosensor.

Source:

red-shifted excitation and emission wavelengths

Source:

supports simultaneous use with CFP/YFP-based ERK FRET biosensors

Source:

compatible with blue light-responsive optogenetic tools

Source:

demonstrated in vitro and in vivo versatility

Compared with AKAR3EV

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Shared frame: source-stated alternative in extracted literature

Strengths here: red-shifted excitation and emission wavelengths; demonstrated versatility in vitro and in vivo; supports simultaneous use with CFP/YFP-based ERK FRET biosensors.

Relative tradeoffs: abstract only demonstrates a PKA biosensor implementation explicitly.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Compared with biosensors

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Shared frame: source-stated alternative in extracted literature

Strengths here: red-shifted excitation and emission wavelengths; demonstrated versatility in vitro and in vivo; supports simultaneous use with CFP/YFP-based ERK FRET biosensors.

Relative tradeoffs: abstract only demonstrates a PKA biosensor implementation explicitly.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Compared with biosensors for active Rho detection

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Shared frame: source-stated alternative in extracted literature

Strengths here: red-shifted excitation and emission wavelengths; demonstrated versatility in vitro and in vivo; supports simultaneous use with CFP/YFP-based ERK FRET biosensors.

Relative tradeoffs: abstract only demonstrates a PKA biosensor implementation explicitly.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Compared with fluorescent protein based reporters and biosensors

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Shared frame: source-stated alternative in extracted literature

Strengths here: red-shifted excitation and emission wavelengths; demonstrated versatility in vitro and in vivo; supports simultaneous use with CFP/YFP-based ERK FRET biosensors.

Relative tradeoffs: abstract only demonstrates a PKA biosensor implementation explicitly.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Compared with FRET

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Shared frame: source-stated alternative in extracted literature

Strengths here: red-shifted excitation and emission wavelengths; demonstrated versatility in vitro and in vivo; supports simultaneous use with CFP/YFP-based ERK FRET biosensors.

Relative tradeoffs: abstract only demonstrates a PKA biosensor implementation explicitly.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Compared with genetically engineered biosensors

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.; The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Shared frame: source-stated alternative in extracted literature

Strengths here: red-shifted excitation and emission wavelengths; demonstrated versatility in vitro and in vivo; supports simultaneous use with CFP/YFP-based ERK FRET biosensors.

Relative tradeoffs: abstract only demonstrates a PKA biosensor implementation explicitly.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors and specifically names AKAR3EV as a previously developed comparator biosensor.

Source:

The abstract contrasts Booster with CFP/YFP-based FRET biosensors, including AKAR3EV and an ERK FRET biosensor based on CFP and YFP. These alternatives are established but have the spectral limitations described by the authors.

Ranked Citations

  1. 1.
    StructuralSource 1ACS Sensors2020Claim 1Claim 2Claim 3

    Extracted from this source document.