Source 1primary paper2021
Toolkit/CaRTRIDGE
CaRTRIDGE
Also known as: Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CaRTRIDGE is a mammalian synthetic biology framework that repurposes CRISPR-associated proteins as translational modulators. In this system, Cas proteins repress or activate translation of mRNAs carrying a cognate Cas-binding RNA motif in the 5′ untranslated region, and the platform can be combined with other Cas-based regulatory layers.
Usefulness & Problems
Why this is useful
CaRTRIDGE provides a way to control gene expression at the translational level using CRISPR-associated proteins rather than only at transcriptional or genome-editing layers. The reported compatibility with Cas-mediated transcriptional regulation supports construction of more complex synthetic circuits with fewer components.
Source:
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
Problem solved
This framework addresses the need for programmable, modular translational control in mammalian cells that can interface with existing CRISPR engineering platforms. It specifically solves the problem of using Cas proteins to regulate translation through 5′-UTR RNA motifs, enabling integrated multi-layer circuit design.
Problem links
Need conditional recombination or state switching
DerivedCaRTRIDGE is a mammalian synthetic biology framework that repurposes CRISPR-associated proteins as translational modulators. In this system, Cas proteins repress or activate translation of mRNAs carrying a cognate Cas-binding RNA motif in the 5′ untranslated region, and the platform can be combined with other Cas-based regulatory layers.
Need controllable genome or transcript editing
DerivedCaRTRIDGE is a mammalian synthetic biology framework that repurposes CRISPR-associated proteins as translational modulators. In this system, Cas proteins repress or activate translation of mRNAs carrying a cognate Cas-binding RNA motif in the 5′ untranslated region, and the platform can be combined with other Cas-based regulatory layers.
Need tighter control over gene expression timing or amplitude
DerivedCaRTRIDGE is a mammalian synthetic biology framework that repurposes CRISPR-associated proteins as translational modulators. In this system, Cas proteins repress or activate translation of mRNAs carrying a cognate Cas-binding RNA motif in the 5′ untranslated region, and the platform can be combined with other Cas-based regulatory layers.
Need tighter control over protein production
DerivedCaRTRIDGE is a mammalian synthetic biology framework that repurposes CRISPR-associated proteins as translational modulators. In this system, Cas proteins repress or activate translation of mRNAs carrying a cognate Cas-binding RNA motif in the 5′ untranslated region, and the platform can be combined with other Cas-based regulatory layers.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
rna motif-dependent translational activationrna motif-dependent translational repressiontranslation controlTranslation ControlTechniques
No technique tags yet.
Target processes
editingrecombinationtranscriptiontranslationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
The core construct logic requires mRNAs containing a Cas-binding RNA motif in the 5′ untranslated region and expression of the corresponding Cas protein. Beyond this motif-dependent 5′-UTR design and mammalian context, the supplied evidence does not provide construct architecture, delivery method, or cofactor requirements.
The provided evidence is limited to a single 2021 source and does not specify which Cas proteins, effect sizes, dynamic ranges, or cell types were tested. Practical performance details, orthogonality, delivery constraints, and independent replication are not established by the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Interconnecting the switches enabled construction of artificial circuits including 60 translational AND gates.
By interconnecting these switches, we designed and built artificial circuits, including 60 translational AND gates.
translational and gates built 60 gates
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug cartridge
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
Source:
compatibilitysupports
Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases synthetic circuit complexity with fewer elements.
Our Cas-mediated translational regulation is compatible with transcriptional regulation by Cas proteins and increases the complexity of synthetic circuits with fewer elements.
Source:
mechanismsupports
A set of Cas proteins can repress or activate translation of mRNAs containing a Cas-binding RNA motif in the 5'-UTR.
We demonstrate that a set of Cas proteins are able to repress (OFF) or activate (ON) the translation of mRNAs that contain a Cas-binding RNA motif in the 5’-UTR.
Source:
repurposing scopesupports
Various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Moreover, we show that various CRISPR-related technologies, including anti-CRISPR and split-Cas9 platforms, can be repurposed to control translation.
Source:
tool proposalsupports
CaRTRIDGE repurposes CRISPR-associated proteins as translational modulators.
Here we propose CaRTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Genomic Engineering) to repurpose CRISPR-associated (Cas) proteins as translational modulators.
Source:
Comparisons
Source-backed strengths
The source literature reports that a set of Cas proteins can both repress and activate translation in an RNA motif-dependent manner. Interconnecting these switches enabled construction of artificial circuits, including 60 translational AND gates, and the system was reported to be compatible with Cas-based transcriptional regulation.
Compared with 4pLRE-cPAOX1
CaRTRIDGE and 4pLRE-cPAOX1 address a similar problem space because they share transcription, translation.
Shared frame: same top-level item type; shared target processes: transcription, translation; shared mechanisms: translation_control
Strengths here: looks easier to implement in practice.
Compared with cell-free biosensors
CaRTRIDGE and cell-free biosensors address a similar problem space because they share recombination, transcription, translation.
Shared frame: same top-level item type; shared target processes: recombination, transcription, translation; shared mechanisms: translation_control
Compared with CRISPR/Cas9 system
CaRTRIDGE and CRISPR/Cas9 system address a similar problem space because they share editing, recombination, transcription, translation.
Shared frame: shared target processes: editing, recombination, transcription, translation; shared mechanisms: translation_control
Relative tradeoffs: appears more independently replicated.
Ranked Citations
- 1.