Source 1review2014Frontiers in Neural Circuits
Toolkit/cell-type specific Cre-driver mouse lines
cell-type specific Cre-driver mouse lines
Also known as: Cre-driver mouse lines
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Now, with the development of optogenetic tools and cell-type specific Cre-driver mouse lines, it has become possible to stimulate cholinergic axons from the basal forebrain (BF) and probe cholinergic synapses in the cortex for the first time.
Usefulness & Problems
Why this is useful
Cell-type specific Cre-driver mouse lines provide genetic access to defined neuronal populations. In the abstract, they are part of the enabling toolkit for selective stimulation of basal forebrain cholinergic axons.; cell-type specific access to cholinergic circuits; supporting selective stimulation experiments in cortex
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Cell-type specific Cre-driver mouse lines provide genetic access to defined neuronal populations. In the abstract, they are part of the enabling toolkit for selective stimulation of basal forebrain cholinergic axons.
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cell-type specific access to cholinergic circuits
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supporting selective stimulation experiments in cortex
Problem solved
They help solve the selectivity problem that previously limited direct study of cholinergic synapses in cortex.; provides cell-type specific targeting needed to selectively interrogate cholinergic axons
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They help solve the selectivity problem that previously limited direct study of cholinergic synapses in cortex.
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provides cell-type specific targeting needed to selectively interrogate cholinergic axons
Problem links
provides cell-type specific targeting needed to selectively interrogate cholinergic axons
LiteratureThey help solve the selectivity problem that previously limited direct study of cholinergic synapses in cortex.
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They help solve the selectivity problem that previously limited direct study of cholinergic synapses in cortex.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
site-specific recombinationTechniques
No technique tags yet.
Target processes
recombinationsignalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
This approach requires appropriate Cre-driver mouse lines and compatible optogenetic experimental components. The supplied text does not specify particular lines or recombinase-dependent constructs.; requires transgenic mouse lines with cell-type specific Cre expression; used alongside optogenetic tools in the review's described approach
The abstract does not state that Cre-driver lines by themselves are sufficient to probe synapses without the accompanying optogenetic tools.; the abstract does not identify specific Cre lines or their targeting scope
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Understanding how cholinergic synapses modulate cortical function had been limited by the inability to selectively activate cholinergic axons, and newer optogenetic tools plus cell-type specific Cre-driver mouse lines made such selective stimulation possible.
The review synthesizes recent work on cell-type specificity of nicotinic signaling in cortex, synaptic mechanisms of cholinergic transmission, and potential functional roles of nicotinic modulation.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug cell-type-specific-cre-driver-mouse-lines
Now, with the development of optogenetic tools and cell-type specific Cre-driver mouse lines, it has become possible to stimulate cholinergic axons from the basal forebrain (BF) and probe cholinergic synapses in the cortex for the first time.
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limitation and enabling advancesupports
Understanding how cholinergic synapses modulate cortical function had been limited by the inability to selectively activate cholinergic axons, and newer optogenetic tools plus cell-type specific Cre-driver mouse lines made such selective stimulation possible.
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review scopesupports
The review synthesizes recent work on cell-type specificity of nicotinic signaling in cortex, synaptic mechanisms of cholinergic transmission, and potential functional roles of nicotinic modulation.
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Comparisons
Source-stated alternatives
The abstract only contrasts this newer genetically targeted approach with earlier behavioral and anatomical data, not with other specific targeting strategies.
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The abstract only contrasts this newer genetically targeted approach with earlier behavioral and anatomical data, not with other specific targeting strategies.
Source-backed strengths
enables cell-type specific experimental access when paired with optogenetic tools
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enables cell-type specific experimental access when paired with optogenetic tools
Compared with CfRhPDE1
cell-type specific Cre-driver mouse lines and CfRhPDE1 address a similar problem space because they share recombination, signaling.
Shared frame: same top-level item type; shared target processes: recombination, signaling; same primary input modality: light
Compared with MNI-L-lac
cell-type specific Cre-driver mouse lines and MNI-L-lac address a similar problem space because they share recombination, signaling.
Shared frame: same top-level item type; shared target processes: recombination, signaling; same primary input modality: light
Compared with photobiomodulation therapy
cell-type specific Cre-driver mouse lines and photobiomodulation therapy address a similar problem space because they share recombination, signaling.
Shared frame: same top-level item type; shared target processes: recombination, signaling; same primary input modality: light
Ranked Citations
- 1.