Toolkit/MNI-L-lac

MNI-L-lac

Construct Pattern·Research·Since 2025

Also known as: 4-methoxy-7-nitroindolinyl-L-lactate

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Here, we report the development of a photocaged derivative of L-lactate, 4-methoxy-7-nitroindolinyl-L-lactate (MNI-L-lac), that releases L-lactate upon illumination.

Usefulness & Problems

Why this is useful

MNI-L-lac is a photocaged derivative of L-lactate that releases L-lactate upon illumination. The abstract presents it as a perturbation reagent for controlled lactate delivery.; perturbing intracellular and extracellular L-lactate concentration; spatiotemporally controlled release of L-lactate in cell culture; probing L-lactate-dependent metabolic and signaling pathways

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MNI-L-lac is a photocaged derivative of L-lactate that releases L-lactate upon illumination. The abstract presents it as a perturbation reagent for controlled lactate delivery.

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perturbing intracellular and extracellular L-lactate concentration

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spatiotemporally controlled release of L-lactate in cell culture

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probing L-lactate-dependent metabolic and signaling pathways

Problem solved

It addresses the lack of tools to perturb intracellular and extracellular L-lactate concentration with spatiotemporal control.; lack of tools to perturb L-lactate concentration intracellularly and extracellularly

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It addresses the lack of tools to perturb intracellular and extracellular L-lactate concentration with spatiotemporal control.

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lack of tools to perturb L-lactate concentration intracellularly and extracellularly

Problem links

lack of tools to perturb L-lactate concentration intracellularly and extracellularly

Literature

It addresses the lack of tools to perturb intracellular and extracellular L-lactate concentration with spatiotemporal control.

Source:

It addresses the lack of tools to perturb intracellular and extracellular L-lactate concentration with spatiotemporal control.

Published Workflows

Synthesis and application of a photocaged-L-lactate for studying the biological roles of L-lactate.

2025

Objective: Develop and apply a photocaged L-lactate reagent for spatiotemporally controlled perturbation of endogenous L-lactate to study metabolic and signaling roles.

Why it works: The workflow uses illumination to release L-lactate with spatiotemporal precision, then checks whether the released molecule is detected by lactate biosensors and can drive a known downstream receptor response.

illumination-triggered release of L-lactateactivation of lactate-responsive signaling through GPR81modulation of adenylyl cyclase and cAMP downstream of released lactatephotocagingcell-culture validationbiosensor-based readoutfunctional receptor activation assay

Stages

  1. 1.
    Tool development(library_build)

    To create a reagent that can perturb L-lactate concentration with optical control.

    Selection: Development of a photocaged derivative of L-lactate that releases L-lactate upon illumination.

  2. 2.
    Cell-culture biosensor validation(confirmatory_validation)

    To validate that illumination of MNI-L-lac produces biologically detectable L-lactate in cell culture.

    Selection: Photoreleased L-lactate elicits responses from extra- and intracellular genetically encoded L-lactate biosensors.

  3. 3.
    Functional signaling application(functional_characterization)

    To demonstrate the utility of MNI-L-lac beyond biosensor detection by showing a downstream signaling consequence of released lactate.

    Selection: Photoreleased L-lactate activates GPR81 with inhibition of adenylyl cyclase activity and decreased cAMP.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A reusable architecture pattern for arranging parts into an engineered system.

Techniques

No technique tags yet.

Target processes

recombinationsignaling

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor

Its use requires illumination to trigger photorelease. The abstract also describes readout using genetically encoded lactate biosensors in cell culture.; requires illumination for photorelease; validated in cell culture in the abstract

The abstract does not establish performance outside cell culture or define broader in vivo applicability.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1application resultsupports2025Source 1needs review

Photoreleased L-lactate from MNI-L-lac activated GPR81, associated with inhibition of adenylyl cyclase activity and decreased cAMP.

To demonstrate the utility of MNI-L-lac, we employed the photorelease of L-lactate to activate G protein-coupled receptor 81 (GPR81), as revealed by the inhibition of adenylyl cyclase activity and concomitant decrease of cAMP.
Claim 2intended usesupports2025Source 1needs review

MNI-L-lac may be useful for perturbing endogenous L-lactate concentration to investigate L-lactate roles in metabolic and signaling pathways.

These results indicate that MNI-L-lac may be useful for perturbing the concentration of endogenous L-lactate in order to investigate L-lactate's roles in metabolic and signaling pathways.
Claim 3tool developmentsupports2025Source 1needs review

MNI-L-lac is a photocaged derivative of L-lactate that releases L-lactate upon illumination.

Here, we report the development of a photocaged derivative of L-lactate, 4-methoxy-7-nitroindolinyl-L-lactate (MNI-L-lac), that releases L-lactate upon illumination.
Claim 4validation resultsupports2025Source 1needs review

In cell culture, photoreleased L-lactate from MNI-L-lac elicited responses in extra- and intracellular genetically encoded L-lactate biosensors.

We validated MNI-L-lac in cell culture by demonstrating that the photorelease of L-lactate elicits a response from genetically encoded extra- and intracellular L-lactate biosensors (eLACCO1, eLACCO2.1, R-iLACCO1.2).

Approval Evidence

1 source4 linked approval claimsfirst-pass slug mni-l-lac
Here, we report the development of a photocaged derivative of L-lactate, 4-methoxy-7-nitroindolinyl-L-lactate (MNI-L-lac), that releases L-lactate upon illumination.

Source:

application resultsupports

Photoreleased L-lactate from MNI-L-lac activated GPR81, associated with inhibition of adenylyl cyclase activity and decreased cAMP.

To demonstrate the utility of MNI-L-lac, we employed the photorelease of L-lactate to activate G protein-coupled receptor 81 (GPR81), as revealed by the inhibition of adenylyl cyclase activity and concomitant decrease of cAMP.

Source:

intended usesupports

MNI-L-lac may be useful for perturbing endogenous L-lactate concentration to investigate L-lactate roles in metabolic and signaling pathways.

These results indicate that MNI-L-lac may be useful for perturbing the concentration of endogenous L-lactate in order to investigate L-lactate's roles in metabolic and signaling pathways.

Source:

tool developmentsupports

MNI-L-lac is a photocaged derivative of L-lactate that releases L-lactate upon illumination.

Here, we report the development of a photocaged derivative of L-lactate, 4-methoxy-7-nitroindolinyl-L-lactate (MNI-L-lac), that releases L-lactate upon illumination.

Source:

validation resultsupports

In cell culture, photoreleased L-lactate from MNI-L-lac elicited responses in extra- and intracellular genetically encoded L-lactate biosensors.

We validated MNI-L-lac in cell culture by demonstrating that the photorelease of L-lactate elicits a response from genetically encoded extra- and intracellular L-lactate biosensors (eLACCO1, eLACCO2.1, R-iLACCO1.2).

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Comparisons

Source-stated alternatives

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

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The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Source-backed strengths

releases L-lactate upon illumination; enables spatiotemporal precision using illumination; was validated with extra- and intracellular genetically encoded L-lactate biosensors; was applied to activate GPR81 with downstream cAMP effects

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releases L-lactate upon illumination

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enables spatiotemporal precision using illumination

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was validated with extra- and intracellular genetically encoded L-lactate biosensors

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was applied to activate GPR81 with downstream cAMP effects

Compared with biosensors

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Shared frame: source-stated alternative in extracted literature

Strengths here: releases L-lactate upon illumination; enables spatiotemporal precision using illumination; was validated with extra- and intracellular genetically encoded L-lactate biosensors.

Source:

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Compared with biosensors for active Rho detection

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Shared frame: source-stated alternative in extracted literature

Strengths here: releases L-lactate upon illumination; enables spatiotemporal precision using illumination; was validated with extra- and intracellular genetically encoded L-lactate biosensors.

Source:

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Compared with fluorescent protein based reporters and biosensors

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Shared frame: source-stated alternative in extracted literature

Strengths here: releases L-lactate upon illumination; enables spatiotemporal precision using illumination; was validated with extra- and intracellular genetically encoded L-lactate biosensors.

Source:

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Compared with genetically engineered biosensors

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Shared frame: source-stated alternative in extracted literature

Strengths here: releases L-lactate upon illumination; enables spatiotemporal precision using illumination; was validated with extra- and intracellular genetically encoded L-lactate biosensors.

Source:

The abstract contrasts MNI-L-lac with genetically encoded lactate biosensors, which are used here as readouts rather than as perturbation tools.

Ranked Citations

  1. 1.
    StructuralSource 1MED2025Claim 1Claim 2Claim 3

    Extracted from this source document.