Source 1primary paper2021Chemical Communications
Toolkit/circularly permuted AsLOV2
circularly permuted AsLOV2
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Circularly permuted AsLOV2 is a re-engineered LOV-domain optogenetic module created by circular permutation of AsLOV2 to enable photoswitchable control over the C-terminus of a peptide. It is reported to function as a light-responsive caging element either alone or in combination with the original AsLOV2 for enhanced caging.
Usefulness & Problems
Why this is useful
This module is useful as an optogenetic protein domain for engineering light-responsive peptides with controllable C-terminal output. The reported ability to use it alone or together with native AsLOV2 suggests utility in building standalone or combinatorial caging designs.
Source:
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Problem solved
It addresses the engineering problem that the original AsLOV2 architecture did not natively provide this reported mode of photoswitchable control over a peptide C-terminus. The circular permutation strategy was introduced specifically to create a module for C-terminal photoswitchable caging.
Source:
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
Implementation is based on a circularly permuted AsLOV2 protein domain used as an optogenetic module for engineering photoswitchable peptides. The evidence supports use as a standalone caging element or in combination with the original AsLOV2, but does not specify construct topology, linker design, cofactors, or expression context.
The supplied evidence does not provide quantitative performance data, wavelength dependence, kinetics, dynamic range, or validation across multiple targets or organisms. Independent replication is not established from the provided source set.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug circularly-permuted-aslov2
the circularly permuted AsLOV2
Source:
application capabilitysupports
Circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging.
Source:
engineering resultsupports
AsLOV2 was re-engineered using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
Source:
field impactsupports
Circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Source:
Comparisons
Source-backed strengths
The main reported strength is that circularly permuted AsLOV2 enables photoswitchable control of a peptide C-terminus through a re-engineered LOV-domain architecture. It is also described as usable in combination with the original AsLOV2 for enhanced caging, indicating potential modularity in optogenetic design.
Source:
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide.
Ranked Citations
- 1.