Source 1review2016Cold Spring Harbor Perspectives in Biology
Toolkit/closed-loop gene networks
closed-loop gene networks
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Closed-loop gene networks are synthetic mammalian gene circuit architectures assembled from gene switches in engineered cells. Reported functions of these complex circuits include event memory, oscillatory protein production, and information-processing behaviors, and microencapsulated mammalian cells carrying such networks have been implanted into mice.
Usefulness & Problems
Why this is useful
These networks are useful as higher-order synthetic biology constructs for programming mammalian cell behavior beyond single-input gene control. The cited literature positions gene switches as modular building blocks for constructing complex circuits that can store past events, generate dynamic outputs, and execute information-processing functions.
Source:
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Source:
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Problem solved
Closed-loop gene networks address the problem of implementing complex, circuit-level control in mammalian cells rather than isolated transgene expression. Specifically, they are described as enabling memory, oscillatory regulation of protein production, and information-processing tasks in engineered mammalian systems.
Source:
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
feedback controlgenetic memoryinformation processingoscillatory regulationtranscriptional gene regulationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuator
The evidence supports implementation in mammalian cells using synthetic gene switches as circuit building blocks. It also supports microencapsulation of engineered mammalian cells for implantation into mice, but it does not specify host cell types, vector systems, promoters, or molecular parts.
The supplied evidence does not provide quantitative performance metrics, specific circuit topologies, component identities, or comparative benchmarking. Independent replication is not established from the provided sources, and validation breadth is limited because the evidence is drawn from a small number of statements in a single cited review source.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Approval Evidence
1 source1 linked approval claimfirst-pass slug closed-loop-gene-networks
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice
Source:
therapeutic applicationsupports
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Source:
Comparisons
Source-backed strengths
A key strength is the reported ability to combine gene switches into complex mammalian circuits with multiple functional behaviors, including memory and oscillation. The available evidence also indicates that engineered mammalian cells containing closed-loop gene networks can be microencapsulated and implanted into mice, supporting at least some in vivo deployment context.
Compared with complex genetic networks
closed-loop gene networks and complex genetic networks address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: information processing
closed-loop gene networks and split luminescent enzyme reconstituted by magnetic stimulus address a similar problem space.
Shared frame: same top-level item type
Ranked Citations
- 1.