Source 1primary paper2025PPR
Toolkit/split luminescent enzyme reconstituted by magnetic stimulus
split luminescent enzyme reconstituted by magnetic stimulus
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is a split luminescent enzyme construct engineered with a magneto-sensitive protein so that enzyme reconstitution is driven by a 50 mT magnetic stimulus. It was described as a component of a magneto-photonic gene circuit in mammalian cells for minimally invasive control of gene expression.
Usefulness & Problems
Why this is useful
The construct provides a way to couple magnetic input to intracellular luminescence generation through stimulus-dependent split-enzyme reconstitution. In the reported context, it supports minimally invasive control architectures for gene expression in mammalian cells and contributes to a combined light-and-magnetic gene circuit.
Source:
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
Problem solved
It addresses the problem of converting a magnetic stimulus into a genetically encoded intracellular signal within mammalian cells. Specifically, it enables magnetic control of split luminescent enzyme assembly at 50 mT as part of a magneto-photonic gene regulation system.
Source:
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
Source:
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
Problem links
providing an internal light source that can be actuated by magnetic stimulus
LiteratureIt provides a way to generate activating light inside cells in response to magnetic input rather than relying only on externally delivered illumination.
Source:
It provides a way to generate activating light inside cells in response to magnetic input rather than relying only on externally delivered illumination.
Published Workflows
Magneto-Photonic Gene Circuit for Minimally Invasive Control of Gene Expression in Mammalian Cells
2025Objective: Engineer a minimally invasive mammalian gene-expression control system that avoids external light delivery by generating intracellular light and coupling it to magnetic actuation.
Why it works: The abstract states that intracellular bioluminescence can bypass inefficient external light delivery, and that combining a luminescent enzyme with a photosensitive transcription factor enables gene-expression control. It further states that magnetic stimulus can reconstitute a split version of the luminescent enzyme, adding magnetic input to the circuit.
bioluminescence-generated intracellular light activates a photosensitive transcription factormagnetic stimulus drives reconstitution of a split luminescent enzymeprotein engineeringmolecular biologygenetic circuit design
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
intracellular bioluminescence generationmagnetic stimulus responsivenesssplit-protein reconstitutionTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Magnetic
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuatorswitch architecture: split
The construct design involves engineering a split luminescent enzyme with a magneto-sensitive protein, consistent with a fusion-based split-protein architecture. The available evidence places its use in mammalian cells and specifies activation by a 50 mT magnetic stimulus, but does not provide construct topology, cofactors, delivery method, or expression details.
The supplied evidence does not identify the specific magneto-sensitive protein, the luminescent enzyme, or quantitative performance metrics such as dynamic range, kinetics, or background reconstitution. Independent replication and validation outside the original 2025 preprint are not provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
magnetic stimulus 50 mT
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The resulting system is presented as a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus.
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The circuit strategy is intended to address limited external light delivery in cells or tissue by generating light directly inside cells with a luminescent enzyme.
the applications of optogenetic systems can be limited by the lack of efficient ways for light delivery inside cells or tissue. Our approach to address this challenge is to harness the power of bioluminescence to produce light directly inside cells using a luminescent enzyme.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
The paper reports development of a fully genetically encoded optogenetic circuit for control of gene expression using intracellular bioluminescence and a photosensitive transcription factor.
Combined with a photosensitive transcription factor, we report the development of a fully genetically encoded optogenetic circuit for control of gene expression.
Approval Evidence
1 source1 linked approval claimfirst-pass slug split-luminescent-enzyme-reconstituted-by-magnetic-stimulus
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
Source:
mechanism or designsupports
A magneto sensitive protein was used to engineer a split luminescent enzyme whose reconstitution is driven by a 50 mT magnetic stimulus.
we utilized a magneto sensitive protein to engineer a split protein version of this luminescent enzyme, where its reconstitution is driven by a 50mT magnetic stimulus
Source:
Comparisons
Source-stated alternatives
The abstract contrasts this design with external light delivery used in standard optogenetic systems. It also implies a non-magnetic intracellular bioluminescence strategy as a related alternative within the same paper.
Source:
The abstract contrasts this design with external light delivery used in standard optogenetic systems. It also implies a non-magnetic intracellular bioluminescence strategy as a related alternative within the same paper.
Source-backed strengths
The reported design directly links a defined magnetic stimulus magnitude, 50 mT, to reconstitution of a split luminescent enzyme. It was presented within a first-of-its-kind gene circuit activated by the combination of light and magnetic stimulus, indicating novelty at the system level.
Source:
resulting in a first-of-its-kind gene circuit activated by a combination of light and magnetic stimulus
Compared with optogenetic
The abstract contrasts this design with external light delivery used in standard optogenetic systems. It also implies a non-magnetic intracellular bioluminescence strategy as a related alternative within the same paper.
Shared frame: source-stated alternative in extracted literature
Strengths here: links magnetic stimulation to reconstitution of a luminescent enzyme; supports combined magnetic and photonic control logic.
Relative tradeoffs: abstract does not name the exact split-enzyme format; abstract does not report reconstitution efficiency or dynamic range.
Source:
The abstract contrasts this design with external light delivery used in standard optogenetic systems. It also implies a non-magnetic intracellular bioluminescence strategy as a related alternative within the same paper.
Compared with optogenetic systems adapted to regulate gene expression
The abstract contrasts this design with external light delivery used in standard optogenetic systems. It also implies a non-magnetic intracellular bioluminescence strategy as a related alternative within the same paper.
Shared frame: source-stated alternative in extracted literature
Strengths here: links magnetic stimulation to reconstitution of a luminescent enzyme; supports combined magnetic and photonic control logic.
Relative tradeoffs: abstract does not name the exact split-enzyme format; abstract does not report reconstitution efficiency or dynamic range.
Source:
The abstract contrasts this design with external light delivery used in standard optogenetic systems. It also implies a non-magnetic intracellular bioluminescence strategy as a related alternative within the same paper.
Ranked Citations
- 1.