Source 1review2023Frontiers in Molecular Biosciences
Toolkit/CRISPR-Cas technology
CRISPR-Cas technology
Also known as: CRISPR, CRISPR-associated proteins
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR-Cas technology comprises CRISPR-associated effector proteins that recognize specific DNA or RNA sequences and cleave them. In the cited review, it is presented primarily as a platform for rapid pathogen nucleic acid detection that leverages Cas trans-cleavage activity together with signal amplification and signal transformation strategies.
Usefulness & Problems
Why this is useful
This technology is useful for molecular diagnosis because sequence-specific nucleic acid recognition and cleavage by Cas proteins can be coupled to diagnostic readouts. The cited review specifically highlights its application to rapid pathogen nucleic acid detection using trans-cleavage-based assay formats.
Source:
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Problem solved
It helps solve the problem of rapidly detecting pathogen nucleic acids in diagnostic settings. The review frames CRISPR-Cas systems as a basis for detection platforms that convert target recognition into measurable signals through signal amplification and signal transformation.
Source:
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Problem links
The summary explicitly states that this item covers rapid pathogen nucleic acid detection based on Cas trans-cleavage activity, which is a direct mechanistic fit for surveillance of emerging bio-threats. It is more relevant to detection than to source attribution.
The summary explicitly states rapid pathogen nucleic-acid detection based on Cas trans-cleavage activity, which is mechanistically relevant to identifying disease causes and emerging pathogens. It could support more informative molecular diagnostics than limited single-analyte tests.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
nuclease-mediated cleavage of dna or rnanuclease-mediated cleavage of dna or rnaPhotocleavagesequence-specific nucleic acid recognitionsequence-specific nucleic acid recognitiontrans-cleavagetrans-cleavageTechniques
No technique tags yet.
Target processes
diagnosticeditingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: builderswitch architecture: cleavage
The cited evidence supports implementation in diagnostic platforms that combine Cas proteins with signal amplification and signal transformation technologies. No specific construct architectures, guide designs, cofactors, organisms, or assay conditions are provided in the supplied evidence.
The supplied evidence is limited to a review-level summary of diagnostic applications and does not provide quantitative performance metrics, specific Cas orthologs, or head-to-head comparisons. It also does not document independent experimental validation, delivery constraints, or editing-specific performance in this evidence set.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
Approval Evidence
2 sources6 linked approval claimsfirst-pass slug crispr-cas-technology
The advent of CRISPR/Cas technology has brought previously inconceivable treatment options to reality.
Source:
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
Source:
capabilitysupports
CRISPR/Cas technology is presented as enabling genomic editing approaches for congenital adrenal hyperplasia.
The advent of CRISPR/Cas technology has brought previously inconceivable treatment options to reality.
Source:
delivery limitationsupports
Delivery of genomic editing reagents to adrenocortical progenitor cells is a major challenge for CAH genomic editing strategies.
There are a number of technologies within reach for CAH, however, delivery of the genomic editing reagents to the elusive adrenocortical progenitor cells remains challenging.
Source:
therapeutic potentialsupports
Genomic editing could repair the defective 21-hydroxylase gene and provide a cure for 21-hydroxylase deficiency.
Genomic editing could repair the defective 21-hydroxylase gene and provide a cure for 21-hydroxylase deficiency, the most common CAH variant, eliminating the current need for constant patient intervention.
Source:
application summarysupports
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Source:
mechanistic summarysupports
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
Source:
review scope summarysupports
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
Source:
Comparisons
Source-backed strengths
A key strength supported by the source is the intrinsic ability of Cas effector proteins to recognize and cut specific DNA or RNA sequences. The review also indicates that trans-cleavage activity has enabled a range of rapid pathogen detection technologies when integrated with amplification and signal conversion methods.
Compared with photoactivatable CRISPR/Cas12a system
CRISPR-Cas technology and photoactivatable CRISPR/Cas12a system address a similar problem space because they share diagnostic, editing.
Shared frame: shared target processes: diagnostic, editing; shared mechanisms: photocleavage
Strengths here: looks easier to implement in practice.
Relative tradeoffs: appears more independently replicated.
Compared with photoactivated CRISPR/Cas12a strategy
CRISPR-Cas technology and photoactivated CRISPR/Cas12a strategy address a similar problem space because they share diagnostic, editing.
Shared frame: shared target processes: diagnostic, editing; shared mechanisms: photocleavage
Strengths here: looks easier to implement in practice.
Compared with transcription activator-like effector nucleases
CRISPR-Cas technology and transcription activator-like effector nucleases address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photocleavage
Ranked Citations
- 1.