Source 1review2019Frontiers in Neuroscience
Toolkit/current/voltage clamp
current/voltage clamp
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Well-established techniques based on current/voltage clamp, optogenetics, calcium imaging, and Micro-Electrode Arrays (MEAs) are proposed for monitoring intra- and extra-cellular responses underlying neuronal dynamics and functional connections.
Usefulness & Problems
Why this is useful
Current/voltage clamp is presented as a well-established technique for monitoring responses underlying neuronal dynamics and functional connections in brain organoids.; monitoring intra-cellular responses; monitoring extra-cellular responses; studying neuronal dynamics; studying functional connections
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Current/voltage clamp is presented as a well-established technique for monitoring responses underlying neuronal dynamics and functional connections in brain organoids.
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monitoring intra-cellular responses
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monitoring extra-cellular responses
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studying neuronal dynamics
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studying functional connections
Problem solved
It helps functionally characterize organoid networks beyond structural description alone.; providing functional readouts of organoid neuronal activity
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It helps functionally characterize organoid networks beyond structural description alone.
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providing functional readouts of organoid neuronal activity
Problem links
providing functional readouts of organoid neuronal activity
LiteratureIt helps functionally characterize organoid networks beyond structural description alone.
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It helps functionally characterize organoid networks beyond structural description alone.
Published Workflows
Objective: Generate and investigate cerebral organoids using a quantitative framework that combines organoid generation, computational assessment, and structural and functional characterization.
Why it works: The review describes a complementary strategy in which generation protocols are followed by computational assessment of formation, organization, and resource uptake, and then by structural and functional characterization of neuronal networks.
organoid formationorganoid organizationneuronal dynamicsfunctional connectivityorganoid generation protocolscomputational modelingstructural characterizationfunctional characterizationcurrent/voltage clampoptogeneticscalcium imagingmicro-electrode arrays
Stages
- 1.Organoid generation protocols(library_build)
This stage establishes the cerebral organoid model system that is subsequently assessed and characterized.
Selection: Generation of cerebral organoid models from human cells using literature protocols.
- 2.Computational assessment of formation, organization, and resource uptake(in_silico_filter)
The review identifies a lack of quantitative framework and presents computational models as a way to assess key organoid properties.
Selection: Assessment of organoid formation, organization, and resource uptake using computational models.
- 3.Structural characterization of brain organoid networks(functional_characterization)
This stage characterizes the organization of organoid neural networks beyond gross formation.
Selection: Experimental approaches for studying single neuron morphology and connections at cellular and sub-cellular resolution.
- 4.Functional monitoring of neuronal dynamics and connections(confirmatory_validation)
Functional assays are used to evaluate neuronal dynamics and connectivity after organoid generation and broader assessment.
Selection: Use of current/voltage clamp, optogenetics, calcium imaging, and MEAs to monitor intra- and extra-cellular responses.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: sensorswitch architecture: multi component
It requires electrophysiology instrumentation and organoid preparations suitable for intracellular or related electrical recording. The abstract does not provide protocol details.; requires electrophysiology capability for current or voltage clamp measurements
The abstract does not indicate that it resolves reproducibility, cellular diversity, or maturation limitations.; the abstract does not specify throughput, spatial coverage, or organoid-specific constraints
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A quantitative framework for generating and investigating cerebral organoids is lacking.
Current/voltage clamp, optogenetics, calcium imaging, and MEAs are proposed as well-established techniques for monitoring responses underlying neuronal dynamics and functional connections in brain organoids.
Computational models are used to assess cerebral organoid formation, organization, and resource uptake.
Approval Evidence
1 source1 linked approval claimfirst-pass slug current-voltage-clamp
Well-established techniques based on current/voltage clamp, optogenetics, calcium imaging, and Micro-Electrode Arrays (MEAs) are proposed for monitoring intra- and extra-cellular responses underlying neuronal dynamics and functional connections.
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functional assay usesupports
Current/voltage clamp, optogenetics, calcium imaging, and MEAs are proposed as well-established techniques for monitoring responses underlying neuronal dynamics and functional connections in brain organoids.
Source:
Comparisons
Source-stated alternatives
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
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The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Source-backed strengths
described as a well-established technique
Source:
described as a well-established technique
Compared with Ca2+ imaging
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with calcium imaging
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with calcium imaging of freely behaving animals
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with imaging
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with imaging surveillance
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with Micro-Electrode Arrays
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with optogenetic functional interrogation
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Compared with optogenetic membrane potential perturbation
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as a well-established technique.
Relative tradeoffs: the abstract does not specify throughput, spatial coverage, or organoid-specific constraints.
Source:
The review also names optogenetics, calcium imaging, and micro-electrode arrays as related functional monitoring approaches.
Ranked Citations
- 1.