Source 1primary paper2022Nature Communications
Toolkit/doxycycline- and light-inducible Cre recombinase
doxycycline- and light-inducible Cre recombinase
Also known as: DiLiCre
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
DiLiCre is an optimized photoactivatable Cre recombinase system described as a doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing. It enables inducible Cre-mediated genome recombination in vivo, including light-induced mutagenesis and positional cell tracing by intravital microscopy.
Usefulness & Problems
Why this is useful
This tool is useful for inducing Cre-lox genome recombination in vivo with combined doxycycline and light control. The reported applications include light-induced mutagenesis and positional cell tracing by intravital microscopy in a mouse model.
Source:
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
Source:
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
Problem solved
DiLiCre addresses the need for inducible in vivo genome editing with optical control in a transgenic mouse context. The source specifically positions it as a doxycycline- and light-inducible system for optogenetic genome editing.
Source:
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
editingrecombinationInput: Light
Implementation Constraints
The available evidence indicates implementation in a mouse transgenic setting and dependence on both doxycycline and light for induction. The provided material does not specify construct architecture, photoreceptor domains, illumination parameters, or delivery details.
The supplied evidence does not provide quantitative performance metrics such as recombination efficiency, background activity, light wavelength, temporal kinetics, or tissue penetration limits. Independent replication is not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
Approval Evidence
1 source5 linked approval claimsfirst-pass slug doxycycline-and-light-inducible-cre-recombinase
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
Source:
application statementsupports
DiLiCre enabled light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy.
we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy
Source:
biological observationsupports
Newly formed HrasV12 mutant cells were observed to move toward the interfollicular dermis in the reported experiments.
These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis.
Source:
engineering resultsupports
The authors engineered an optimized photoactivatable Cre recombinase system called DiLiCre.
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
Source:
performance statementsupports
DiLiCre is described as an efficient photoactivatable Cre recombinase mouse model.
Together, we develop an efficient photoactivatable Cre recombinase mouse model
Source:
utility statementsupports
The model is presented as a powerful genome-editing tool for biological and biomedical research.
illustrate how this model is a powerful genome-editing tool for biological and biomedical research
Source:
Comparisons
Source-backed strengths
The system was reported as an optimized photoactivatable Cre recombinase and was demonstrated in vivo in a mouse model. Reported use cases include light-induced mutagenesis and positional cell tracing, and the study observed newly formed HrasV12 mutant cells moving toward the interfollicular dermis.
Source:
Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre).
Source:
Together, we develop an efficient photoactivatable Cre recombinase mouse model
Ranked Citations
- 1.