Source 1primary paper2017CU Scholar (University of Colorado Boulder)
Toolkit/droplet microfluidic platform
droplet microfluidic platform
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The droplet microfluidic platform is an assay method for screening and separating cell populations based on the in vivo fluorescence response of expressed biosensors after addition of an exogenous analyte. It was applied to HeLa-cell genetic linker libraries for genetically encoded Zn2+ sensors to assess library diversity and detect response heterogeneity.
Usefulness & Problems
Why this is useful
This platform is useful for functional screening of biosensor-expressing cell populations using an in vivo fluorescence readout rather than only sequence-level or bulk measurements. In the cited application, it enabled assessment of diversity in HeLa-cell linker libraries for Zn2+ sensor variants and supported separation of populations according to analyte-induced biosensor behavior.
Problem solved
The method addresses the problem of screening and separating heterogeneous cell populations on the basis of analyte-responsive biosensor performance in living cells. In the reported use case, it helped identify heterogeneity among targeted genetically encoded Zn2+ biosensors in HeLa cells.
Problem links
A droplet microfluidic platform is plausibly useful for scaling screening or assay development, which could support higher-throughput surveillance workflows. However, the provided summary is about fluorescence biosensor-based cell screening rather than direct pathogen detection.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
cell population separation based on analyte-induced biosensor responsecell population sorting/separation based on analyte-induced biosensor responsefluorescence-based functional readoutfluorescence-based functional readoutmicrofluidic droplet compartmentalizationmicrofluidic droplet compartmentalizationTarget processes
recombinationselectionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The reported implementation involves a droplet microfluidic instrument and requires expressed fluorescence-based biosensors whose in vivo response can be measured after addition of an exogenous analyte. The documented application used HeLa-cell genetic linker libraries for a family of genetically encoded Zn2+ sensors, but the evidence does not specify construct architecture, droplet chemistry, or optical hardware details.
The supplied evidence documents development and use in HeLa cells with fluorescence-based Zn2+ biosensors, but it does not provide broader validation across other cell types, analytes, or biosensor classes. No quantitative performance metrics, throughput values, sorting purity, droplet composition, or instrument specifications are provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug droplet-microfluidic-platform
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
Source:
library screening usesupports
The droplet microfluidic instrument was used to screen and assess diversity in HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Subsequently, the instrument is used to screen and assess diversity in a number of HeLa-cell based genetic linker libraries for a family of genetically-encoded Zn2+ sensors.
Source:
method developmentsupports
The authors developed a droplet microfluidic platform for screening and separating cell populations based on the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte.
Source:
screening observationsupports
Screening with the droplet microfluidic instrument revealed increased heterogeneity among targeted Zn2+ biosensors in HeLa cells.
Screening with this instrument reveals increased heterogeneity in an array of targeted Zn2+ biosensorsin HeLa cells that helps shed light on the complexities of these sensors in different chemical environments.
Source:
Comparisons
Source-backed strengths
The platform directly links exogenous analyte addition to an in vivo fluorescence-based functional readout in compartmentalized cell populations. Source claims indicate that it was sufficient to screen HeLa-cell genetic linker libraries and reveal increased heterogeneity among targeted Zn2+ biosensors.
Compared with FLIPR
droplet microfluidic platform and FLIPR address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Compared with fluorescence recovery after photobleaching
droplet microfluidic platform and fluorescence recovery after photobleaching address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Compared with open-source microplate reader
droplet microfluidic platform and open-source microplate reader address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Ranked Citations
- 1.