Source 1primary paper2026MED
Toolkit/electrochemical impedance spectroscopy
electrochemical impedance spectroscopy
Also known as: EIS
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Using electrochemical impedance spectroscopy, the device exhibits a linear response over the range of 62.5-1000 nM tetracycline.
Usefulness & Problems
Why this is useful
Electrochemical impedance spectroscopy is the readout method used to measure the sensor response to tetracycline across the reported concentration range.; impedimetric readout of tetracycline concentration; quantifying sensor response; EIS is the assay readout used to detect whether α-syntrophin binds the gold electrode-bound photoswitchable peptide. The abstract states that it provides both qualitative and quantitative sensor information.; detecting binding of α-syntrophin to the surface-bound photoswitchable peptide; qualitative and quantitative sensor readout
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Electrochemical impedance spectroscopy is the readout method used to measure the sensor response to tetracycline across the reported concentration range.
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impedimetric readout of tetracycline concentration
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quantifying sensor response
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EIS is the assay readout used to detect whether α-syntrophin binds the gold electrode-bound photoswitchable peptide. The abstract states that it provides both qualitative and quantitative sensor information.
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detecting binding of α-syntrophin to the surface-bound photoswitchable peptide
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qualitative and quantitative sensor readout
Problem solved
It enables quantitative readout of the nanocomposite-functionalized interdigitated electrode sensor.; providing the measurement method for the disposable tetracycline sensor; It converts the light-controlled binding event into a measurable electrochemical signal.; provides an electrochemical readout of the light-controlled protein-peptide interaction
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It enables quantitative readout of the nanocomposite-functionalized interdigitated electrode sensor.
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providing the measurement method for the disposable tetracycline sensor
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It converts the light-controlled binding event into a measurable electrochemical signal.
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provides an electrochemical readout of the light-controlled protein-peptide interaction
Problem links
provides an electrochemical readout of the light-controlled protein-peptide interaction
LiteratureIt converts the light-controlled binding event into a measurable electrochemical signal.
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It converts the light-controlled binding event into a measurable electrochemical signal.
providing the measurement method for the disposable tetracycline sensor
LiteratureIt enables quantitative readout of the nanocomposite-functionalized interdigitated electrode sensor.
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It enables quantitative readout of the nanocomposite-functionalized interdigitated electrode sensor.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract supports that the biosensor must be interrogated by electrochemical impedance spectroscopy instrumentation.; requires an impedimetric sensor device compatible with electrochemical impedance spectroscopy; The abstract supports the need for a gold electrode-bound peptide and EIS measurement setup.; requires a gold electrode-bound peptide interface; requires electrochemical instrumentation
The abstract does not indicate that EIS itself controls binding; control is provided by the photoswitchable peptide and light.; the abstract only supports this assay in the context of the gold electrode-bound peptide system
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successCell-freeapplication demo
electrochemical impedance spectroscopy
Inferred from claim claim_3 during normalization. Electrochemical impedance spectroscopy detected binding between the gold electrode-bound peptide in its cis photostationary state and α-syntrophin across a wide range of concentrations. Derived from claim claim_3.
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target concentration range(wide range of concentrations)
Supporting Sources
Source 2primary paper2018Biosensors and Bioelectronics
Ranked Claims
The combination of biocompatible low-cost materials and simple fabrication supports single-use deployment and scalability.
Using electrochemical impedance spectroscopy, the device shows a linear response for tetracycline from 62.5 to 1000 nM with a limit of detection of 42 nM and a limit of quantification of 138 nM.
limit of detection 42 nMlimit of quantification 138 nMlinear response range lower bound 62.5 nMlinear response range upper bound 1000 nM
The sensor detects tetracycline in lake, tap, and bottled water with linear responses across the same concentration range.
linear response range lower bound 62.5 nMlinear response range upper bound 1000 nM
The sensor shows strong selectivity over ampicillin, amoxicillin, cephalexin, doxycycline, penicillin, and glucose.
A disposable non-enzymatic impedimetric biosensor using interdigitated electrodes functionalized with Mn-doped ZnS-chitosan nanocomposite can rapidly and selectively detect tetracycline.
Electrochemical impedance spectroscopy detected binding between the gold electrode-bound peptide in its cis photostationary state and α-syntrophin across a wide range of concentrations.
target concentration range wide range of concentrations
An azobenzene photoswitch incorporated into the peptide backbone enables reversible switching between a trans state lacking secondary structure and a cis state with antiparallel β-strand geometry.
The biosensor has high thermal stability of the cis photostationary state and enables regenerable on-off control of binding using light.
The probe in its random trans photostationary state does not bind α-syntrophin.
A light-driven photoswitchable peptide-based biosensor modeled on the nNOS β-finger was used to detect and control interaction with α-syntrophin.
Approval Evidence
2 sources2 linked approval claimsfirst-pass slug electrochemical-impedance-spectroscopy
Using electrochemical impedance spectroscopy, the device exhibits a linear response over the range of 62.5-1000 nM tetracycline.
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Electrochemical impedance spectroscopy (EIS) is used to successfully detect the interaction between the gold electrode bound peptide in its cis photostationary state and a wide range of concentrations of α-syntrophin protein.
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performancesupports
Using electrochemical impedance spectroscopy, the device shows a linear response for tetracycline from 62.5 to 1000 nM with a limit of detection of 42 nM and a limit of quantification of 138 nM.
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assay resultsupports
Electrochemical impedance spectroscopy detected binding between the gold electrode-bound peptide in its cis photostationary state and α-syntrophin across a wide range of concentrations.
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Comparisons
Source-stated alternatives
No alternative assay modalities are directly compared in the abstract.
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No alternative assay modalities are directly compared in the abstract.
Source-backed strengths
successfully detects binding in the cis state; supports measurement across a wide range of target concentrations; distinguishes binding and non-binding photoswitch states
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successfully detects binding in the cis state
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supports measurement across a wide range of target concentrations
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distinguishes binding and non-binding photoswitch states
Compared with cyclic voltammetry
electrochemical impedance spectroscopy and cyclic voltammetry address a similar problem space.
Shared frame: same top-level item type; same primary input modality: chemical
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with multicomponent, ligand-functionalized microarrays
electrochemical impedance spectroscopy and multicomponent, ligand-functionalized microarrays address a similar problem space.
Shared frame: same top-level item type; same primary input modality: chemical
Strengths here: appears more independently replicated; looks easier to implement in practice.
electrochemical impedance spectroscopy and time-resolved imaging of nucleoid spatial distribution after drug perturbation address a similar problem space.
Shared frame: same top-level item type; same primary input modality: chemical
Strengths here: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.
- 2.