Source 1primary paper2022BIO-PROTOCOL
Toolkit/multicomponent, ligand-functionalized microarrays
multicomponent, ligand-functionalized microarrays
Also known as: Patterned Substrate of Mobile and Immobile Ligands
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Multicomponent, ligand-functionalized microarrays are a patterned substrate assay method for individual living cells that spatially segregates distinct ligand presentations to enable simultaneous monitoring of receptor activation and downstream signaling. The method was developed to probe clustering-dependent EphA2 signal transduction.
Usefulness & Problems
Why this is useful
This assay is useful for dissecting how spatially organized ligand presentation influences receptor activation and signaling within single living cells. The source indicates that the protocol is potentially applicable beyond EphA2 to multiple cell signaling systems and microbe-host interactions.
Source:
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
Source:
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
Source:
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Source:
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
Problem solved
The method addresses the problem of measuring receptor activation and signaling under spatially segregated ligand conditions in the same live-cell assay. It was specifically developed to study the effects of receptor clustering on EphA2 signaling transduction.
Source:
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
Source:
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
Problem links
Microarray fabrication and assay formats can support parallelized testing of many chemical conditions or ligands, which is relevant to reducing low-throughput manual workflows. It is more of a high-throughput screening aid than a synthesis automation solution.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
clustering-dependent signaling modulationclustering-dependent signaling modulationligand-mediated receptor activationligand-mediated receptor activationspatial segregation of ligand presentationspatial segregation of mobile and immobile ligand presentationTechniques
Functional AssayTarget processes
signalingInput: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Implementation involves fabrication of multicomponent, ligand-functionalized microarrays on patterned substrates for live-cell measurements. The provided evidence does not specify ligand chemistries, substrate materials, imaging modalities, or construct design details.
The supplied evidence does not report quantitative performance metrics, throughput, sensitivity, or comparative benchmarking against other live-cell signaling assays. Independent replication and validation outside the reported EphA2-focused application are not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug multicomponent-ligand-functionalized-microarrays
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays
Source:
application scopesupports
The protocol is potentially applicable to multiple cell signaling systems or microbe-host interactions.
It is potentially applicable to multiple cell signaling systems, or microbe/host interactions.
Source:
application scopesupports
The protocol was developed to study effects of clustering on EphA2 signaling transduction.
This protocol has been developed to study the effects of clustering on EphA2 signaling transduction.
Source:
method capabilitysupports
The described method fabricates multicomponent, ligand-functionalized microarrays for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Here, we describe a method to fabricate multicomponent, ligand-functionalized microarrays, for spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells.
Source:
method capabilitysupports
The protocol allows functionalization of both mobile membrane corrals and immobile polymers with selective ligands and microscopic monitoring of cognate receptor activation at the cell membrane interface.
This protocol uniquely allows for functionalization of both mobile membrane corrals and immobile polymers with selective ligands, as well as microscopic monitoring of cognate receptor activation at the cell membrane interface.
Source:
Comparisons
Source-backed strengths
A key strength is the ability to fabricate multicomponent, ligand-functionalized microarrays that support spatially segregated and simultaneous monitoring of receptor activation and signaling in individual living cells. The protocol is also presented as potentially extensible to multiple signaling systems or microbe-host interaction contexts.
Compared with IRAP-pHluorin translocation assay
multicomponent, ligand-functionalized microarrays and IRAP-pHluorin translocation assay address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Strengths here: looks easier to implement in practice.
multicomponent, ligand-functionalized microarrays and light-induced Fourier transform infrared (FTIR) difference spectroscopy address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Strengths here: looks easier to implement in practice.
multicomponent, ligand-functionalized microarrays and root-specific transcriptomic dataset comparison for ethylene responses address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Ranked Citations
- 1.