Source 1primary paper2020Analytical Chemistry
Toolkit/exonuclease III assisted nucleic acid cascade recycling amplification
exonuclease III assisted nucleic acid cascade recycling amplification
Also known as: EXO III assisted nucleic acid cascade recycling amplification
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Exonuclease III assisted nucleic acid cascade recycling amplification is an engineered nucleic acid signal amplification method used within a near-infrared light-activatable circuit. In the cited implementation, it is combined with a photocontrollable nucleic acid displacement reaction and upconversion nanoparticles to enable spatiotemporally controllable amplified mRNA imaging in living cancer cells.
Usefulness & Problems
Why this is useful
This method is useful because it couples enzymatic nucleic acid recycling amplification to optical triggering, allowing amplification to be turned on by near-infrared light rather than proceeding constitutively. The reported application is signal-amplified mRNA imaging in selected living cancer cells with spatiotemporal control.
Source:
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Problem solved
It addresses the problem of achieving controllable nucleic acid signal amplification for intracellular mRNA imaging, specifically adding near-infrared light control over when and where amplification occurs. The cited work frames this as a spatiotemporally controllable signal amplification strategy in living cancer cells.
Source:
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Problem links
Need precise spatiotemporal control with light input
DerivedExonuclease III assisted nucleic acid cascade recycling amplification is an engineering method for light-triggered nucleic acid signal amplification. In the cited implementation, it is integrated with a photocontrollable nucleic acid displacement reaction and upconversion nanoparticles to enable near-infrared (NIR) activated, spatiotemporally controllable amplified mRNA imaging in living cancer cells.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
exonuclease-assisted target recycling amplificationexonuclease-assisted target recycling amplificationnucleic acid strand displacementnucleic acid strand displacementphotocontrol of amplification activationphotocontrol of amplification activationupconversion nanoparticle-mediated near-infrared activationupconversion nanoparticle-mediated nir activationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builder
The reported implementation requires exonuclease III, a photocontrollable nucleic acid displacement module, and upconversion nanoparticles to achieve NIR activation. The evidence supports use in living cancer cells for mRNA imaging, but it does not specify construct sequences, irradiation parameters, delivery methods, or nanoparticle composition.
The supplied evidence is limited to a single cited implementation and does not report quantitative performance metrics such as gain, sensitivity, background suppression, kinetics, or multiplexing capacity. Independent replication, broader organismal validation, and detailed operating constraints are not provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
Approval Evidence
1 source1 linked approval claimfirst-pass slug exonuclease-iii-assisted-nucleic-acid-cascade-recycling-amplification
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification
Source:
method compositionsupports
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
Source:
Comparisons
Source-backed strengths
The main demonstrated strength is integration into a NIR-triggered amplification process for spatiotemporally controllable, signal-amplified mRNA imaging in living cancer cells. The method composition explicitly combines photocontrollable nucleic acid displacement, EXO III-assisted cascade recycling amplification, and upconversion nanoparticles, supporting modular control of activation.
exonuclease III assisted nucleic acid cascade recycling amplification and Method for efficient synthesis of phycocyanobilin in cultured mammalian cells address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
exonuclease III assisted nucleic acid cascade recycling amplification and photocontrollable nucleic acid cascade recycling amplification address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: nucleic acid strand displacement; same primary input modality: light
Compared with photocontrollable nucleic acid displacement reaction
exonuclease III assisted nucleic acid cascade recycling amplification and photocontrollable nucleic acid displacement reaction address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: upconversion nanoparticle-mediated nir activation; same primary input modality: light
Ranked Citations
- 1.