Source 1primary paper2020Analytical Chemistry
Toolkit/photocontrollable nucleic acid displacement reaction
photocontrollable nucleic acid displacement reaction
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photocontrollable nucleic acid displacement reaction is a light-gated nucleic acid engineering method used within a near-infrared-activatable cascade recycling amplification system. In the cited implementation, it is integrated with exonuclease III-assisted nucleic acid amplification and upconversion nanoparticles to enable spatiotemporally controllable, signal-amplified mRNA imaging in selected living cancer cells.
Usefulness & Problems
Why this is useful
This method is useful because it allows near-infrared light to control when nucleic acid amplification is triggered, adding spatiotemporal regulation to intracellular mRNA imaging. The reported system couples photocontrol with cascade recycling amplification to increase signal output in living cancer cells.
Source:
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Problem solved
The method addresses the problem of triggering nucleic acid signal amplification in a controllable manner for mRNA imaging in living cells. Specifically, it provides a near-infrared-activatable route to initiate amplification only upon light input, rather than relying on constitutive activation.
Source:
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Problem links
Need precise spatiotemporal control with light input
DerivedPhotocontrollable nucleic acid displacement reaction is a light-gated nucleic acid engineering strategy used as part of a near-infrared (NIR)-activatable cascade recycling amplification system. In the cited implementation, it is integrated with exonuclease III-assisted nucleic acid amplification and upconversion nanoparticles to trigger signal-amplified mRNA imaging in living cancer cells with spatiotemporal control.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
exonuclease iii-assisted cascade recycling amplificationexonuclease iii-assisted cascade recycling amplificationlight-triggered activationlight-triggered activationstrand displacementstrand displacementupconversion nanoparticle-mediated near-infrared activationupconversion nanoparticle-mediated nir activationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builder
The reported implementation integrates a photocontrollable nucleic acid displacement reaction with exonuclease III-assisted nucleic acid cascade recycling amplification and upconversion nanoparticles. Near-infrared light is the stated input used to control and trigger the amplification process, and the application context is living cancer cell mRNA imaging.
The supplied evidence is limited to a single reported implementation for mRNA imaging in selected living cancer cells. The evidence does not specify sequence design rules, quantitative performance metrics, generality across targets or cell types, or independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
Approval Evidence
1 source1 linked approval claimfirst-pass slug photocontrollable-nucleic-acid-displacement-reaction
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification
Source:
method compositionsupports
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
Source:
Comparisons
Source-backed strengths
The cited study demonstrated a NIR light-triggered signal amplification process in selected living cancer cells for spatiotemporally controllable, signal-amplified mRNA imaging. Its design combines photocontrollable nucleic acid displacement, exonuclease III-assisted cascade recycling amplification, and upconversion nanoparticles in a single activatable platform.
photocontrollable nucleic acid displacement reaction and exonuclease III assisted nucleic acid cascade recycling amplification address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: upconversion nanoparticle-mediated nir activation; same primary input modality: light
photocontrollable nucleic acid displacement reaction and Method for efficient synthesis of phycocyanobilin in cultured mammalian cells address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
photocontrollable nucleic acid displacement reaction and photocontrollable nucleic acid cascade recycling amplification address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: exonuclease iii-assisted cascade recycling amplification; same primary input modality: light
Ranked Citations
- 1.