Source 1primary paper2016Proceedings of the National Academy of Sciences
Toolkit/expansion microscopy
expansion microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Within the bouquets, expansion microscopy resolved many individual striosomal fibers tightly intertwined with the dopamine-containing dendrites and also with afferents labeled by glutamatergic, GABAergic, and cholinergic markers and markers for astrocytic cells and fibers and connexin 43 puncta.
Usefulness & Problems
Why this is useful
Expansion microscopy was used to resolve individual striosomal fibers within the bouquet and their close association with dopamine-containing dendrites and other labeled elements.; resolving fine anatomical relationships within striosome-dendron bouquets; visualizing intertwined fibers, dendrites, and labeled afferents at high spatial detail
Source:
Expansion microscopy was used to resolve individual striosomal fibers within the bouquet and their close association with dopamine-containing dendrites and other labeled elements.
Source:
resolving fine anatomical relationships within striosome-dendron bouquets
Source:
visualizing intertwined fibers, dendrites, and labeled afferents at high spatial detail
Problem solved
It helps distinguish tightly intertwined cellular and fiber components in a compact anatomical structure.; improves resolution of densely interwoven neural elements within the bouquet structure
Source:
It helps distinguish tightly intertwined cellular and fiber components in a compact anatomical structure.
Source:
improves resolution of densely interwoven neural elements within the bouquet structure
Problem links
improves resolution of densely interwoven neural elements within the bouquet structure
LiteratureIt helps distinguish tightly intertwined cellular and fiber components in a compact anatomical structure.
Source:
It helps distinguish tightly intertwined cellular and fiber components in a compact anatomical structure.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescence-based anatomical colocalization of labeled tissue componentsphysical sample expansion for enhanced spatial resolutionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract indicates a microscopy workflow with marker-labeled afferents, astrocytic markers, and connexin 43 puncta in tissue samples.; requires labeled neural elements and microscopy-compatible tissue preparation
The abstract does not indicate that it measures circuit activity or causal function.; the abstract only supports structural resolution, not functional readout
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Expansion microscopy resolved many individual striosomal fibers tightly intertwined with dopamine-containing dendrites and with afferents labeled by glutamatergic, GABAergic, cholinergic, astrocytic, and connexin 43 markers within the bouquets.
Retrograde tracing showed that clustered nigral cell bodies associated with the bouquets project to the striatum as part of the classic nigrostriatal pathway.
Approval Evidence
1 source1 linked approval claimfirst-pass slug expansion-microscopy
Within the bouquets, expansion microscopy resolved many individual striosomal fibers tightly intertwined with the dopamine-containing dendrites and also with afferents labeled by glutamatergic, GABAergic, and cholinergic markers and markers for astrocytic cells and fibers and connexin 43 puncta.
Source:
method capabilitysupports
Expansion microscopy resolved many individual striosomal fibers tightly intertwined with dopamine-containing dendrites and with afferents labeled by glutamatergic, GABAergic, cholinergic, astrocytic, and connexin 43 markers within the bouquets.
Source:
Comparisons
Source-stated alternatives
The abstract also mentions retrograde tracing, which addresses projection identity rather than the same fine structural resolution task.
Source:
The abstract also mentions retrograde tracing, which addresses projection identity rather than the same fine structural resolution task.
Source-backed strengths
resolved many individual striosomal fibers within a dense circuit motif
Source:
resolved many individual striosomal fibers within a dense circuit motif
Compared with retrograde tracing
The abstract also mentions retrograde tracing, which addresses projection identity rather than the same fine structural resolution task.
Shared frame: source-stated alternative in extracted literature
Strengths here: resolved many individual striosomal fibers within a dense circuit motif.
Relative tradeoffs: the abstract only supports structural resolution, not functional readout.
Source:
The abstract also mentions retrograde tracing, which addresses projection identity rather than the same fine structural resolution task.
Ranked Citations
- 1.