Source 1primary paper2016Proceedings of the National Academy of Sciences
Toolkit/retrograde tracing
retrograde tracing
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Retrograde tracing showed that these clustered cell bodies in turn project to the striatum as part of the classic nigrostriatal pathway.
Usefulness & Problems
Why this is useful
Retrograde tracing was used to show that clustered nigral cell bodies associated with the bouquet project to the striatum.; identifying projection targets of clustered nigral cell bodies associated with bouquets; linking bouquet-associated neurons to the nigrostriatal pathway
Source:
Retrograde tracing was used to show that clustered nigral cell bodies associated with the bouquet project to the striatum.
Source:
identifying projection targets of clustered nigral cell bodies associated with bouquets
Source:
linking bouquet-associated neurons to the nigrostriatal pathway
Problem solved
It connects the bouquet-associated nigral cell clusters to the classic nigrostriatal pathway.; establishes whether bouquet-associated nigral cell bodies project back to the striatum
Source:
It connects the bouquet-associated nigral cell clusters to the classic nigrostriatal pathway.
Source:
establishes whether bouquet-associated nigral cell bodies project back to the striatum
Problem links
establishes whether bouquet-associated nigral cell bodies project back to the striatum
LiteratureIt connects the bouquet-associated nigral cell clusters to the classic nigrostriatal pathway.
Source:
It connects the bouquet-associated nigral cell clusters to the classic nigrostriatal pathway.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract supports use of a retrograde anatomical tracing approach to identify projection-defined neurons.; requires anatomical tracing of projection-defined neurons
The abstract does not show that retrograde tracing alone resolves local microanatomical intertwining or functional signaling.; the abstract does not provide finer mechanistic or functional information beyond projection identity
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Expansion microscopy resolved many individual striosomal fibers tightly intertwined with dopamine-containing dendrites and with afferents labeled by glutamatergic, GABAergic, cholinergic, astrocytic, and connexin 43 markers within the bouquets.
Retrograde tracing showed that clustered nigral cell bodies associated with the bouquets project to the striatum as part of the classic nigrostriatal pathway.
Approval Evidence
1 source1 linked approval claimfirst-pass slug retrograde-tracing
Retrograde tracing showed that these clustered cell bodies in turn project to the striatum as part of the classic nigrostriatal pathway.
Source:
projection mappingsupports
Retrograde tracing showed that clustered nigral cell bodies associated with the bouquets project to the striatum as part of the classic nigrostriatal pathway.
Source:
Comparisons
Source-stated alternatives
The abstract pairs this with expansion microscopy, which addresses local structural resolution rather than long-range projection identity.
Source:
The abstract pairs this with expansion microscopy, which addresses local structural resolution rather than long-range projection identity.
Source-backed strengths
supports projection-level circuit assignment in the abstract
Source:
supports projection-level circuit assignment in the abstract
Compared with expansion microscopy
The abstract pairs this with expansion microscopy, which addresses local structural resolution rather than long-range projection identity.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports projection-level circuit assignment in the abstract.
Relative tradeoffs: the abstract does not provide finer mechanistic or functional information beyond projection identity.
Source:
The abstract pairs this with expansion microscopy, which addresses local structural resolution rather than long-range projection identity.
Compared with microscopy
The abstract pairs this with expansion microscopy, which addresses local structural resolution rather than long-range projection identity.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports projection-level circuit assignment in the abstract.
Relative tradeoffs: the abstract does not provide finer mechanistic or functional information beyond projection identity.
Source:
The abstract pairs this with expansion microscopy, which addresses local structural resolution rather than long-range projection identity.
Ranked Citations
- 1.