Source 1review2023Frontiers in Molecular Biosciences
Toolkit/fluorescence method
fluorescence method
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The fluorescence method is a signal transformation modality used in CRISPR-Cas pathogen nucleic acid diagnostic platforms. In the cited context, Cas effector proteins recognize and cleave specific DNA or RNA targets, and fluorescence is combined with signal amplification and transformation technologies to report detection.
Usefulness & Problems
Why this is useful
This method is useful as a readout strategy for CRISPR-Cas-based molecular diagnosis of pathogen nucleic acids. The available evidence indicates that fluorescence serves as part of signal transformation workflows coupled to Cas-mediated target recognition and cleavage.
Source:
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Problem solved
It helps convert Cas protein activity on specific pathogen-derived DNA or RNA sequences into a detectable diagnostic signal. The cited literature specifically places fluorescence within platforms designed for rapid pathogen nucleic acid detection.
Source:
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Problem links
This item is a signal readout modality for CRISPR-based nucleic acid detection, so it could support rapid molecular assays for surveillance of known targets. Its relevance is mainly as an assay component rather than a full surveillance solution.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescent signal transformationnuclease cleavagesequence-specific nucleic acid recognitiontrans-cleavageTechniques
Functional AssayTarget processes
diagnosticImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The available evidence indicates implementation within CRISPR-Cas diagnostic systems that include Cas proteins plus signal amplification and signal transformation components. No specific construct architecture, reporter molecule, reaction conditions, cofactor requirements, or instrumentation details are provided in the supplied sources.
The supplied evidence does not specify which fluorescent chemistries, reporter designs, Cas proteins, wavelengths, limits of detection, or pathogen panels were validated for this fluorescence method. Independent replication and comparative performance against other readout modalities are not established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
Approval Evidence
1 source1 linked approval claimfirst-pass slug fluorescence-method
in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Source:
application summarysupports
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Source:
Comparisons
Source-backed strengths
The method is supported as part of CRISPR-Cas diagnostic platforms that leverage the intrinsic sequence specificity of Cas effectors for DNA or RNA recognition and cleavage. It is also explicitly described as compatible with signal amplification and signal transformation technologies in pathogen detection workflows.
Compared with magnetic nanoparticle biosensors
fluorescence method and magnetic nanoparticle biosensors address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic
Compared with magnetic resonance elastography
fluorescence method and magnetic resonance elastography address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic
Compared with qRT-PCR
fluorescence method and qRT-PCR address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic
Relative tradeoffs: appears more independently replicated.
Ranked Citations
- 1.