Source 1review2020Current Opinion in Biotechnology
Toolkit/fluorescent sensors
fluorescent sensors
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we review techniques for detecting the intracellular metabolic state with fluorescent sensors
Usefulness & Problems
Why this is useful
Fluorescent sensors are described as techniques for detecting the intracellular metabolic state. In this review context, they are positioned as monitoring tools for central carbon metabolism during microbial production.; detecting intracellular metabolic state; monitoring metabolism during fermentation; supporting dynamic control of microbial production systems
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Fluorescent sensors are described as techniques for detecting the intracellular metabolic state. In this review context, they are positioned as monitoring tools for central carbon metabolism during microbial production.
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detecting intracellular metabolic state
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monitoring metabolism during fermentation
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supporting dynamic control of microbial production systems
Problem solved
They help reveal intracellular metabolic conditions that change over time and matter for cofactor regeneration and flux control. This supports efforts to maintain an appropriate cellular state during production.; provides a way to sense changing intracellular metabolic conditions relevant to central carbon metabolism
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They help reveal intracellular metabolic conditions that change over time and matter for cofactor regeneration and flux control. This supports efforts to maintain an appropriate cellular state during production.
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provides a way to sense changing intracellular metabolic conditions relevant to central carbon metabolism
Problem links
provides a way to sense changing intracellular metabolic conditions relevant to central carbon metabolism
LiteratureThey help reveal intracellular metabolic conditions that change over time and matter for cofactor regeneration and flux control. This supports efforts to maintain an appropriate cellular state during production.
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They help reveal intracellular metabolic conditions that change over time and matter for cofactor regeneration and flux control. This supports efforts to maintain an appropriate cellular state during production.
Published Workflows
Flux controlling technology for central carbon metabolism for efficient microbial bio-production
2020Objective: Fine-tune central carbon metabolism flux distribution in microbial bioproduction by combining intracellular state detection with dynamic control.
Why it works: The review abstract links changing fermentation conditions to the need for dynamic control, and pairs intracellular-state detection with optogenetic actuation as a way to maintain an appropriate cellular state while fine-tuning flux distribution.
detection of intracellular metabolic stateoptogenetic control of central carbon metabolismfluorescent sensingoptogenetic control
Stages
- 1.Intracellular metabolic state detection(functional_characterization)
This stage exists to reveal intracellular metabolic conditions relevant to central carbon metabolism and cofactor status before or during dynamic control.
Selection: Detection of intracellular metabolic state with fluorescent sensors
- 2.Dynamic flux control of central carbon metabolism(functional_characterization)
This stage exists because intracellular and extracellular conditions change over time during fermentation, making dynamic control necessary to maintain an appropriate cellular state.
Selection: Control of central carbon metabolism flux with optogenetic tools
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescence-based detectionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Use of these tools requires fluorescent sensor systems compatible with the microorganism and fermentation setting. The abstract does not specify particular sensor designs, reporters, or instrumentation.; requires fluorescent sensing capability in the microbial system
The abstract does not indicate that sensing alone changes flux or improves production without a linked control strategy. It also does not specify coverage of all relevant metabolites or cofactors.; the abstract does not specify which metabolites or cofactors each sensor can measure
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Fluorescent sensors are used to detect the intracellular metabolic state in the context of central carbon metabolism and microbial bioproduction.
Approval Evidence
1 source1 linked approval claimfirst-pass slug fluorescent-sensors
Here, we review techniques for detecting the intracellular metabolic state with fluorescent sensors
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tool use casesupports
Fluorescent sensors are used to detect the intracellular metabolic state in the context of central carbon metabolism and microbial bioproduction.
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Comparisons
Source-stated alternatives
The abstract contrasts sensing with optogenetic tools for control rather than naming alternative sensing platforms. Adjacent source leads suggest genetically encoded fluorescent sensors as a more specific related class, but the anchor abstract itself stays broad.
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The abstract contrasts sensing with optogenetic tools for control rather than naming alternative sensing platforms. Adjacent source leads suggest genetically encoded fluorescent sensors as a more specific related class, but the anchor abstract itself stays broad.
Source-backed strengths
enables detection of intracellular metabolic state
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enables detection of intracellular metabolic state
Compared with optogenetic
The abstract contrasts sensing with optogenetic tools for control rather than naming alternative sensing platforms. Adjacent source leads suggest genetically encoded fluorescent sensors as a more specific related class, but the anchor abstract itself stays broad.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables detection of intracellular metabolic state.
Relative tradeoffs: the abstract does not specify which metabolites or cofactors each sensor can measure.
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The abstract contrasts sensing with optogenetic tools for control rather than naming alternative sensing platforms. Adjacent source leads suggest genetically encoded fluorescent sensors as a more specific related class, but the anchor abstract itself stays broad.
Ranked Citations
- 1.