Source 1primary paper2011Nature Communications
Toolkit/KFERQ-PA-mCherry1
KFERQ-PA-mCherry1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
High-signal enrichment leads and related item candidates identify a photoactivable/photoconvertible CMA reporter built around a KFERQ-targeting motif fused to PA-mCherry1, and explicitly name the reporter variant as KFERQ-PA-mCherry1.
Usefulness & Problems
Why this is useful
This construct is described as a photoactivable/photoconvertible fluorescent reporter for monitoring chaperone-mediated autophagy. It is built around a KFERQ-targeting motif fused to PA-mCherry1.; tracking chaperone-mediated autophagy in living cells; monitoring CMA activity with a fluorescent reporter
Source:
This construct is described as a photoactivable/photoconvertible fluorescent reporter for monitoring chaperone-mediated autophagy. It is built around a KFERQ-targeting motif fused to PA-mCherry1.
Source:
tracking chaperone-mediated autophagy in living cells
Source:
monitoring CMA activity with a fluorescent reporter
Problem solved
It provides a way to track CMA activity using a fluorescent reporter rather than relying only on indirect pathway measurements.; provides a fluorescent reporter for CMA activity
Source:
It provides a way to track CMA activity using a fluorescent reporter rather than relying only on indirect pathway measurements.
Source:
provides a fluorescent reporter for CMA activity
Problem links
provides a fluorescent reporter for CMA activity
LiteratureIt provides a way to track CMA activity using a fluorescent reporter rather than relying only on indirect pathway measurements.
Source:
It provides a way to track CMA activity using a fluorescent reporter rather than relying only on indirect pathway measurements.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Selection / EnrichmentTarget processes
selectionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Use of the reporter requires the engineered KFERQ-PA-mCherry1 construct and fluorescence-based imaging or readout in living cells.; requires a KFERQ motif fused to PA-mCherry1 as the reporter construct
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper describes a photoconvertible fluorescent reporter for tracking chaperone-mediated autophagy.
A photoconvertible fluorescent reporter to track chaperone-mediated autophagy
Approval Evidence
1 source1 linked approval claimfirst-pass slug kferq-pa-mcherry1
High-signal enrichment leads and related item candidates identify a photoactivable/photoconvertible CMA reporter built around a KFERQ-targeting motif fused to PA-mCherry1, and explicitly name the reporter variant as KFERQ-PA-mCherry1.
Source:
tool purposesupports
The paper describes a photoconvertible fluorescent reporter for tracking chaperone-mediated autophagy.
A photoconvertible fluorescent reporter to track chaperone-mediated autophagy
Source:
Comparisons
Source-backed strengths
explicitly described as photoconvertible/photoactivable fluorescent reporter for CMA
Source:
explicitly described as photoconvertible/photoactivable fluorescent reporter for CMA
Compared with CfRhPDE1
KFERQ-PA-mCherry1 and CfRhPDE1 address a similar problem space because they share selection.
Shared frame: same top-level item type; shared target processes: selection; same primary input modality: light
Compared with CheRiff
KFERQ-PA-mCherry1 and CheRiff address a similar problem space because they share selection.
Shared frame: same top-level item type; shared target processes: selection; same primary input modality: light
Compared with luciferin-luciferase pair
KFERQ-PA-mCherry1 and luciferin-luciferase pair address a similar problem space because they share selection.
Shared frame: same top-level item type; shared target processes: selection; same primary input modality: light
Ranked Citations
- 1.