Source 1primary paper2015PLoS ONE
Toolkit/LANS
LANS
Also known as: light-activated nuclear shuttle, LOV/NLS switch
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LANS is a light-activated nuclear shuttle, or LOV/NLS switch, that uses blue light to control nuclear localization by uncaging a nuclear localization signal. It has been used to regulate transcription in yeast and to manipulate nuclear localization and cell fate in the C. elegans embryo, including light-dependent control of the native transcription factor LIN-1.
Usefulness & Problems
Why this is useful
LANS provides spatial and temporal control of nuclear localization in vivo through light input. This makes it useful for experimentally controlling transcriptional regulation and cell fate decisions in specific cells and time windows.
Source:
By inserting LANS into the C. elegans lin-1 locus using Cas9-triggered homologous recombination, we demonstrated control of cell fate via light-dependent manipulation of a native transcription factor.
Source:
demonstrating that it can be used to control gene transcription in yeast
Source:
the switch, referred to as LANS (light-activated nuclear shuttle), functions in the C. elegans embryo and allows for control of nuclear localization in individual cells
Source:
We conclude that LANS can be a valuable experimental method for spatial and temporal control of nuclear localization in vivo.
Problem solved
LANS addresses the problem of controlling when and where proteins enter the nucleus. The cited work specifically shows its use for light-dependent control of gene transcription in yeast and for manipulating nuclear localization and cell fate in individual C. elegans embryonic cells.
Source:
By inserting LANS into the C. elegans lin-1 locus using Cas9-triggered homologous recombination, we demonstrated control of cell fate via light-dependent manipulation of a native transcription factor.
Source:
demonstrating that it can be used to control gene transcription in yeast
Source:
the switch, referred to as LANS (light-activated nuclear shuttle), functions in the C. elegans embryo and allows for control of nuclear localization in individual cells
Problem links
Need conditional recombination or state switching
DerivedLANS is a light-activated nuclear shuttle, also described as a LOV/NLS switch, that uses blue light to control nuclear localization. It has been applied to regulate transcription in yeast and to manipulate nuclear localization and cell fate in the C. elegans embryo, including light-dependent control of the native transcription factor LIN-1.
Need inducible protein relocalization or recruitment
DerivedLANS is a light-activated nuclear shuttle, also described as a LOV/NLS switch, that uses blue light to control nuclear localization. It has been applied to regulate transcription in yeast and to manipulate nuclear localization and cell fate in the C. elegans embryo, including light-dependent control of the native transcription factor LIN-1.
Need precise spatiotemporal control with light input
DerivedLANS is a light-activated nuclear shuttle, also described as a LOV/NLS switch, that uses blue light to control nuclear localization. It has been applied to regulate transcription in yeast and to manipulate nuclear localization and cell fate in the C. elegans embryo, including light-dependent control of the native transcription factor LIN-1.
Need tighter control over gene expression timing or amplitude
DerivedLANS is a light-activated nuclear shuttle, also described as a LOV/NLS switch, that uses blue light to control nuclear localization. It has been applied to regulate transcription in yeast and to manipulate nuclear localization and cell fate in the C. elegans embryo, including light-dependent control of the native transcription factor LIN-1.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
blue-light-triggered nuclear importblue-light-triggered nuclear importlight-induced conformational switchinglight-induced conformational switchingsteric caging and uncaging of a nuclear localization signalsteric caging and uncaging of a nuclear localization signalTechniques
No technique tags yet.
Target processes
localizationrecombinationtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: uncaging
LANS is a multi-component light-responsive construct described as a LOV/NLS switch, indicating a fusion architecture that couples a LOV photosensory element to an NLS. Reported implementations include use in yeast, function in the C. elegans embryo, and insertion into the endogenous lin-1 locus by genome engineering.
The supplied evidence does not report quantitative performance metrics such as import kinetics, dynamic range, reversibility, or background nuclear localization in the dark. Validation is described for yeast and C. elegans embryo applications, but broader organismal or assay coverage is not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Insertion of LANS into the C. elegans lin-1 locus enabled control of cell fate through light-dependent manipulation of a native transcription factor.
By inserting LANS into the C. elegans lin-1 locus using Cas9-triggered homologous recombination, we demonstrated control of cell fate via light-dependent manipulation of a native transcription factor.
LANS can be used to control gene transcription in yeast.
demonstrating that it can be used to control gene transcription in yeast
LANS functions in the C. elegans embryo and allows control of nuclear localization in individual cells.
the switch, referred to as LANS (light-activated nuclear shuttle), functions in the C. elegans embryo and allows for control of nuclear localization in individual cells
In the dark, the LOV/NLS switch adopts a closed conformation that sterically blocks the NLS motif, and blue light activation unfolds the C-terminus to free the NLS and direct nuclear import.
In the dark, the switch adopts a closed conformation that sterically blocks the NLS motif. Upon activation with blue light the C-terminus of the protein unfolds, freeing the NLS to direct the protein to the nucleus.
LANS is a valuable experimental method for spatial and temporal control of nuclear localization in vivo.
We conclude that LANS can be a valuable experimental method for spatial and temporal control of nuclear localization in vivo.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug lans
the switch, referred to as LANS (light-activated nuclear shuttle)
Source:
applicationsupports
Insertion of LANS into the C. elegans lin-1 locus enabled control of cell fate through light-dependent manipulation of a native transcription factor.
By inserting LANS into the C. elegans lin-1 locus using Cas9-triggered homologous recombination, we demonstrated control of cell fate via light-dependent manipulation of a native transcription factor.
Source:
applicationsupports
LANS can be used to control gene transcription in yeast.
demonstrating that it can be used to control gene transcription in yeast
Source:
applicationsupports
LANS functions in the C. elegans embryo and allows control of nuclear localization in individual cells.
the switch, referred to as LANS (light-activated nuclear shuttle), functions in the C. elegans embryo and allows for control of nuclear localization in individual cells
Source:
mechanismsupports
In the dark, the LOV/NLS switch adopts a closed conformation that sterically blocks the NLS motif, and blue light activation unfolds the C-terminus to free the NLS and direct nuclear import.
In the dark, the switch adopts a closed conformation that sterically blocks the NLS motif. Upon activation with blue light the C-terminus of the protein unfolds, freeing the NLS to direct the protein to the nucleus.
Source:
utilitysupports
LANS is a valuable experimental method for spatial and temporal control of nuclear localization in vivo.
We conclude that LANS can be a valuable experimental method for spatial and temporal control of nuclear localization in vivo.
Source:
Comparisons
Source-backed strengths
The reported strengths are blue-light-dependent nuclear import and in vivo operation in both yeast and the C. elegans embryo. The tool was also integrated into the endogenous C. elegans lin-1 locus to control cell fate via a native transcription factor, supporting utility beyond ectopic reporter contexts.
Compared with Cry2/CIB
LANS and Cry2/CIB address a similar problem space because they share localization, recombination, transcription.
Shared frame: same top-level item type; shared target processes: localization, recombination, transcription; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with iLID/SspB
LANS and iLID/SspB address a similar problem space because they share localization, recombination, transcription.
Shared frame: same top-level item type; shared target processes: localization, recombination, transcription; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with UVR8/UVR8
LANS and UVR8/UVR8 address a similar problem space because they share localization, recombination, transcription.
Shared frame: same top-level item type; shared target processes: localization, recombination, transcription; same primary input modality: light
Ranked Citations
- 1.
Derived from 5 linked claims. Example evidence: By inserting LANS into the C. elegans lin-1 locus using Cas9-triggered homologous recombination, we demonstrated control of cell fate via light-dependent manipulation of a native transcription factor.