Source 1review2017Proceedings of the Royal Society A Mathematical Physical and Engineering Sciences
Toolkit/Light-addressable potentiometric sensor
Light-addressable potentiometric sensor
Also known as: LAPS, Light-addressable potentiometric sensors
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light-addressable potentiometric sensors (LAPS) ... use photocurrent measurements at electrolyte-insulator-semiconductor substrates for spatio-temporal imaging of electrical potentials and impedance.
Usefulness & Problems
Why this is useful
LAPS uses photocurrent measurements at electrolyte-insulator-semiconductor substrates to image electrical potentials and related chemical signals in space and time. The abstract describes both sensor-array interrogation and biological imaging uses.; spatio-temporal imaging of electrical potentials; interrogation of sensor arrays; detection of ions; label-free detection of charged molecules; enzyme-based biosensing; imaging dynamic concentration changes in microfluidic channels
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LAPS uses photocurrent measurements at electrolyte-insulator-semiconductor substrates to image electrical potentials and related chemical signals in space and time. The abstract describes both sensor-array interrogation and biological imaging uses.
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spatio-temporal imaging of electrical potentials
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interrogation of sensor arrays
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detection of ions
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label-free detection of charged molecules
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enzyme-based biosensing
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imaging dynamic concentration changes in microfluidic channels
Problem solved
It enables label-free, spatially resolved detection of ions, charged biomolecules, and biologically relevant concentration changes. It also supports imaging of extracellular potentials and cell-associated signals.; provides label-free electrical readout of chemical and biological signals; enables spatially resolved sensing on electrolyte-insulator-semiconductor substrates
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It enables label-free, spatially resolved detection of ions, charged biomolecules, and biologically relevant concentration changes. It also supports imaging of extracellular potentials and cell-associated signals.
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provides label-free electrical readout of chemical and biological signals
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enables spatially resolved sensing on electrolyte-insulator-semiconductor substrates
Problem links
This is a real biosensing platform for spatiotemporal imaging of electrical potentials and impedance, so it could support exploratory measurements of biophysical states in living samples. However, the provided evidence supports conventional potentiometric sensing rather than direct detection of quantum phenomena.
enables spatially resolved sensing on electrolyte-insulator-semiconductor substrates
LiteratureIt enables label-free, spatially resolved detection of ions, charged biomolecules, and biologically relevant concentration changes. It also supports imaging of extracellular potentials and cell-associated signals.
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It enables label-free, spatially resolved detection of ions, charged biomolecules, and biologically relevant concentration changes. It also supports imaging of extracellular potentials and cell-associated signals.
provides label-free electrical readout of chemical and biological signals
LiteratureIt enables label-free, spatially resolved detection of ions, charged biomolecules, and biologically relevant concentration changes. It also supports imaging of extracellular potentials and cell-associated signals.
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It enables label-free, spatially resolved detection of ions, charged biomolecules, and biologically relevant concentration changes. It also supports imaging of extracellular potentials and cell-associated signals.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The method requires an electrolyte-insulator-semiconductor substrate and photocurrent measurement instrumentation. The abstract also implies optical addressing as part of the light-addressable measurement setup.; requires photocurrent measurement; requires electrolyte-insulator-semiconductor substrates
The abstract does not show that LAPS alone solves all biological imaging needs or provides molecular specificity beyond charged or enzyme-coupled readouts. It also does not establish exact resolution or throughput limits.; the abstract does not specify exact performance limits or comparative weaknesses
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Applications described for these techniques include detection of ions, label-free detection of charged molecules such as DNA and proteins, and enzyme-based biosensors.
LAPS and SPIM have been used for interrogation of sensor arrays and imaging of biological systems.
Imaging applications described in the review include temporal imaging of extracellular potentials, dynamic concentration changes in microfluidic channels, and lateral imaging of cell surface charges and cell metabolism.
LAPS and SPIM use photocurrent measurements at electrolyte-insulator-semiconductor substrates for spatio-temporal imaging of electrical potentials and impedance.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug light-addressable-potentiometric-sensor
Light-addressable potentiometric sensors (LAPS) ... use photocurrent measurements at electrolyte-insulator-semiconductor substrates for spatio-temporal imaging of electrical potentials and impedance.
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application scopesupports
Applications described for these techniques include detection of ions, label-free detection of charged molecules such as DNA and proteins, and enzyme-based biosensors.
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application scopesupports
LAPS and SPIM have been used for interrogation of sensor arrays and imaging of biological systems.
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biological imaging scopesupports
Imaging applications described in the review include temporal imaging of extracellular potentials, dynamic concentration changes in microfluidic channels, and lateral imaging of cell surface charges and cell metabolism.
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review scope summarysupports
LAPS and SPIM use photocurrent measurements at electrolyte-insulator-semiconductor substrates for spatio-temporal imaging of electrical potentials and impedance.
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Comparisons
Source-stated alternatives
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
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The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Source-backed strengths
supports spatio-temporal imaging; supports label-free detection of charged molecules; has broad application range from ions to biological imaging
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supports spatio-temporal imaging
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supports label-free detection of charged molecules
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has broad application range from ions to biological imaging
Compared with imaging
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports spatio-temporal imaging; supports label-free detection of charged molecules; has broad application range from ions to biological imaging.
Relative tradeoffs: the abstract does not specify exact performance limits or comparative weaknesses.
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The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Compared with imaging surveillance
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports spatio-temporal imaging; supports label-free detection of charged molecules; has broad application range from ions to biological imaging.
Relative tradeoffs: the abstract does not specify exact performance limits or comparative weaknesses.
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The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Compared with microscopy
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports spatio-temporal imaging; supports label-free detection of charged molecules; has broad application range from ions to biological imaging.
Relative tradeoffs: the abstract does not specify exact performance limits or comparative weaknesses.
Source:
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Compared with Scanning photo-induced impedance microscopy
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports spatio-temporal imaging; supports label-free detection of charged molecules; has broad application range from ions to biological imaging.
Relative tradeoffs: the abstract does not specify exact performance limits or comparative weaknesses.
Source:
The review directly pairs LAPS with scanning photo-induced impedance microscopy as a related modality. The abstract does not provide a broader comparison against other imaging platforms.
Ranked Citations
- 1.