Source 1review2018Advanced Science
Toolkit/light-controllable designer cells
light-controllable designer cells
Also known as: light-sensitive mammalian cells, optogenetically engineered cells
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light-controllable designer cells are optogenetically engineered mammalian cells whose behavior is regulated by light. The available evidence supports their use as a precise and noninvasive control modality in therapeutic synthetic biology.
Usefulness & Problems
Why this is useful
These constructs are useful because they enable precise, noninvasive regulation of engineered mammalian cell behavior. The cited evidence places this capability in the context of therapeutic synthetic biology.
Source:
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Problem solved
They help address the need for external control over engineered mammalian cells in therapeutic synthetic biology. The specific problem supported by the evidence is achieving precise and noninvasive regulation of cell behavior.
Source:
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Problem links
Need precise spatiotemporal control with light input
DerivedLight-controllable designer cells are optogenetically engineered mammalian cells whose behavior is regulated by light. The available evidence supports their use as a precise and noninvasive control modality in therapeutic synthetic biology.
Need tighter control over protein production
DerivedLight-controllable designer cells are optogenetically engineered mammalian cells whose behavior is regulated by light. The available evidence supports their use as a precise and noninvasive control modality in therapeutic synthetic biology.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
translationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The available evidence identifies these systems as engineered mammalian cells that combine optogenetics with therapeutic synthetic biology. No specific photoreceptor, wavelength, construct architecture, expression strategy, or delivery method is provided in the supplied evidence.
The source notes that current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed, indicating unresolved limitations in the field. However, the provided evidence does not specify the exact bottlenecks, molecular implementations, or application-specific constraints for this tool class.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug light-controllable-designer-cells
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Source:
application scopesupports
Combining optogenetics with therapeutic synthetic biology has enabled engineering of light-controllable designer cells whose behavior can be regulated precisely and noninvasively.
Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light-controllable designer cells, whose behavior can be regulated precisely and noninvasively.
Source:
bottleneck summarysupports
Current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.
Source:
translational scopesupports
The review summarizes translational applications of optogenetically engineered cells spanning in vitro basic research to in vivo light-controlled gene therapy.
Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light-controlled gene therapy.
Source:
Comparisons
Source-backed strengths
The reported strengths are precision and noninvasiveness of light-based control over mammalian designer cells. The evidence supports the general concept of regulating cell behavior with light, but does not provide quantitative performance data.
Compared with 4pLRE-cPAOX1
light-controllable designer cells and 4pLRE-cPAOX1 address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Compared with blue-light-activated DNA template ON switch
light-controllable designer cells and blue-light-activated DNA template ON switch address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Compared with triple brake design
light-controllable designer cells and triple brake design address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Ranked Citations
- 1.