Source 1primary paper2018Journal of Extracellular Vesicles
Toolkit/MISEV2014 guidelines
MISEV2014 guidelines
Also known as: Minimal Information for Studies of Extracellular Vesicles, MISEV2014
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years.
Usefulness & Problems
Why this is useful
MISEV2014 is the earlier ISEV guideline framework for extracellular vesicle studies referenced as the basis for the 2018 update.; prior standardized guidance for extracellular vesicle studies
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MISEV2014 is the earlier ISEV guideline framework for extracellular vesicle studies referenced as the basis for the 2018 update.
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prior standardized guidance for extracellular vesicle studies
Problem solved
It provided an initial minimum-information standard for the EV field.; providing an earlier minimum-information framework for EV research
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It provided an initial minimum-information standard for the EV field.
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providing an earlier minimum-information framework for EV research
Problem links
providing an earlier minimum-information framework for EV research
LiteratureIt provided an initial minimum-information standard for the EV field.
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It provided an initial minimum-information standard for the EV field.
Published Workflows
Objective: Document specific extracellular vesicle-associated functional activities with sufficient reporting and characterization rigor.
Why it works: The abstract states that specific EV functions should not be assigned from crude, potentially contaminated, heterogeneous preparations alone, and that MISEV2018 provides protocols, steps, and a checklist to document EV-associated functional activities.
distinguishing EV-associated functions from effects attributable to crude, contaminated, or heterogeneous preparationssuggested protocolsstepwise documentationchecklist-based reporting
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
No mechanism tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Operational role: sensor. Implementation mode: genetically encoded. Cofactor status: cofactor requirement unknown.
superseded in part by MISEV2018 according to the abstract
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
MISEV2018 includes suggested protocols, steps, and a checklist for documenting specific EV-associated functional activities.
The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
MISEV2018 updates the MISEV2014 guidelines based on evolution of collective knowledge over the previous four years.
We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years.
Assigning specific functions to extracellular vesicles or EV subtypes requires reporting information beyond mere description of function in crude, potentially contaminated, heterogeneous preparations.
ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation
Approval Evidence
1 source1 linked approval claimfirst-pass slug misev2014-guidelines
The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years.
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guideline updatesupports
MISEV2018 updates the MISEV2014 guidelines based on evolution of collective knowledge over the previous four years.
We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years.
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Comparisons
Source-stated alternatives
MISEV2018 is presented as the updated version.
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MISEV2018 is presented as the updated version.
Source-backed strengths
served as the basis for the 2018 update
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served as the basis for the 2018 update
Compared with MISEV2018 guidelines
MISEV2018 is presented as the updated version.
Shared frame: source-stated alternative in extracted literature
Strengths here: served as the basis for the 2018 update.
Relative tradeoffs: superseded in part by MISEV2018 according to the abstract.
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MISEV2018 is presented as the updated version.
Ranked Citations
- 1.