Source 1primary paper2018Journal of Extracellular Vesicles
Toolkit/MISEV2018 guidelines
MISEV2018 guidelines
Also known as: Minimal Information for Studies of Extracellular Vesicles 2018, MISEV2018
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
Usefulness & Problems
Why this is useful
MISEV2018 is a reporting and study-guidance framework for extracellular vesicle research. The abstract states that it includes suggested protocols, steps, and a checklist for documenting EV-associated functional activities.; standardized reporting of extracellular vesicle studies; documenting EV-associated functional activities
Source:
MISEV2018 is a reporting and study-guidance framework for extracellular vesicle research. The abstract states that it includes suggested protocols, steps, and a checklist for documenting EV-associated functional activities.
Source:
standardized reporting of extracellular vesicle studies
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documenting EV-associated functional activities
Problem solved
It addresses the difficulty of interpreting EV function claims when preparations are impure, heterogeneous, or poorly characterized.; insufficient reporting for assigning functions to extracellular vesicles; lack of standardized documentation for EV preparation and characterization
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It addresses the difficulty of interpreting EV function claims when preparations are impure, heterogeneous, or poorly characterized.
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insufficient reporting for assigning functions to extracellular vesicles
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lack of standardized documentation for EV preparation and characterization
Problem links
insufficient reporting for assigning functions to extracellular vesicles
LiteratureIt addresses the difficulty of interpreting EV function claims when preparations are impure, heterogeneous, or poorly characterized.
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It addresses the difficulty of interpreting EV function claims when preparations are impure, heterogeneous, or poorly characterized.
lack of standardized documentation for EV preparation and characterization
LiteratureIt addresses the difficulty of interpreting EV function claims when preparations are impure, heterogeneous, or poorly characterized.
Source:
It addresses the difficulty of interpreting EV function claims when preparations are impure, heterogeneous, or poorly characterized.
Published Workflows
Objective: Document specific extracellular vesicle-associated functional activities with sufficient reporting and characterization rigor.
Why it works: The abstract states that specific EV functions should not be assigned from crude, potentially contaminated, heterogeneous preparations alone, and that MISEV2018 provides protocols, steps, and a checklist to document EV-associated functional activities.
distinguishing EV-associated functions from effects attributable to crude, contaminated, or heterogeneous preparationssuggested protocolsstepwise documentationchecklist-based reporting
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
No mechanism tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Using MISEV2018 requires the ability to report EV preparation, characterization, and functional-study details beyond a simple functional claim from crude material.; requires reporting information beyond description of function in crude, potentially contaminated, heterogeneous preparations
The abstract does not claim that the guideline itself solves the underlying biological uncertainty around exosome-specific biogenesis and release mechanisms.; does not resolve limited knowledge of specific molecular machineries of exosome biogenesis and release
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
MISEV2018 includes suggested protocols, steps, and a checklist for documenting specific EV-associated functional activities.
The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
MISEV2018 updates the MISEV2014 guidelines based on evolution of collective knowledge over the previous four years.
We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years.
Assigning specific functions to extracellular vesicles or EV subtypes requires reporting information beyond mere description of function in crude, potentially contaminated, heterogeneous preparations.
ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation
Approval Evidence
1 source3 linked approval claimsfirst-pass slug misev2018-guidelines
The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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guideline contentsupports
MISEV2018 includes suggested protocols, steps, and a checklist for documenting specific EV-associated functional activities.
The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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guideline updatesupports
MISEV2018 updates the MISEV2014 guidelines based on evolution of collective knowledge over the previous four years.
We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years.
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reporting requirementsupports
Assigning specific functions to extracellular vesicles or EV subtypes requires reporting information beyond mere description of function in crude, potentially contaminated, heterogeneous preparations.
ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation
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Comparisons
Source-stated alternatives
The source presents MISEV2014 as the prior version that MISEV2018 updates.
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The source presents MISEV2014 as the prior version that MISEV2018 updates.
Source-backed strengths
provides suggested protocols and steps; includes a checklist summarizing key points; updates prior MISEV2014 guidance
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provides suggested protocols and steps
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includes a checklist summarizing key points
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updates prior MISEV2014 guidance
Compared with MISEV2014 guidelines
The source presents MISEV2014 as the prior version that MISEV2018 updates.
Shared frame: source-stated alternative in extracted literature
Strengths here: provides suggested protocols and steps; includes a checklist summarizing key points; updates prior MISEV2014 guidance.
Relative tradeoffs: does not resolve limited knowledge of specific molecular machineries of exosome biogenesis and release.
Source:
The source presents MISEV2014 as the prior version that MISEV2018 updates.
Ranked Citations
- 1.