Source 1primary paper2008PLoS ONE
Toolkit/mKikGR
mKikGR
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates.
Usefulness & Problems
Why this is useful
mKikGR is a monomeric photoswitchable fluorescent protein derived from KikGR. The abstract states that it has high photostability and switching rates and can support single-molecule localization.; super-resolution imaging; single-molecule localization; tagging molecular species in cells
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mKikGR is a monomeric photoswitchable fluorescent protein derived from KikGR. The abstract states that it has high photostability and switching rates and can support single-molecule localization.
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super-resolution imaging
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single-molecule localization
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tagging molecular species in cells
Problem solved
The paper positions mKikGR as addressing common limitations of available photoswitchable proteins, specifically fast photobleaching, slow photoswitching, and oligomerization. It is suggested as suitable for super-resolution imaging.; reduces oligomerization relative to previously reported KikGR; addresses fast photobleaching and slow photoswitching limitations of available photoswitchable proteins
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The paper positions mKikGR as addressing common limitations of available photoswitchable proteins, specifically fast photobleaching, slow photoswitching, and oligomerization. It is suggested as suitable for super-resolution imaging.
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reduces oligomerization relative to previously reported KikGR
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addresses fast photobleaching and slow photoswitching limitations of available photoswitchable proteins
Problem links
addresses fast photobleaching and slow photoswitching limitations of available photoswitchable proteins
LiteratureThe paper positions mKikGR as addressing common limitations of available photoswitchable proteins, specifically fast photobleaching, slow photoswitching, and oligomerization. It is suggested as suitable for super-resolution imaging.
Source:
The paper positions mKikGR as addressing common limitations of available photoswitchable proteins, specifically fast photobleaching, slow photoswitching, and oligomerization. It is suggested as suitable for super-resolution imaging.
reduces oligomerization relative to previously reported KikGR
LiteratureThe paper positions mKikGR as addressing common limitations of available photoswitchable proteins, specifically fast photobleaching, slow photoswitching, and oligomerization. It is suggested as suitable for super-resolution imaging.
Source:
The paper positions mKikGR as addressing common limitations of available photoswitchable proteins, specifically fast photobleaching, slow photoswitching, and oligomerization. It is suggested as suitable for super-resolution imaging.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
Its reported characterization involved molecular cloning, spectroscopy, and single-molecule imaging experiments. Use as an imaging tag therefore requires fluorescence imaging instrumentation capable of photoswitching and localization measurements.; requires molecular cloning and spectroscopic characterization; used in single-molecule imaging experiments for demonstrated localization performance
The abstract does not establish performance in specific live-animal, therapeutic, or non-imaging applications. It also does not provide enough detail to judge all limitations relative to other photoconvertible proteins.; abstract does not specify exact mutation set or full performance tradeoffs
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Individual mKikGR proteins can be localized with precision better than 10 nanometers in single-molecule imaging experiments.
individual mKikGR proteins can be localized with a precision of better than 10 nanometers
localization precision 10 nanometers
mKikGR is suggested to be suitable for super-resolution imaging.
suggesting their suitability for super-resolution imaging
mKikGR displays high photostability and switching rates.
mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates
mKikGR is a monomeric version of KikGR.
mKikGR, a monomeric version of the previously reported KikGR
Approval Evidence
1 source4 linked approval claimsfirst-pass slug mkikgr
Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates.
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application capabilitysupports
Individual mKikGR proteins can be localized with precision better than 10 nanometers in single-molecule imaging experiments.
individual mKikGR proteins can be localized with a precision of better than 10 nanometers
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application suitabilitysupports
mKikGR is suggested to be suitable for super-resolution imaging.
suggesting their suitability for super-resolution imaging
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tool propertysupports
mKikGR displays high photostability and switching rates.
mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates
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tool propertysupports
mKikGR is a monomeric version of KikGR.
mKikGR, a monomeric version of the previously reported KikGR
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Comparisons
Source-stated alternatives
The abstract explicitly contrasts mKikGR with the previously reported KikGR and with photoswitchable proteins currently available at the time.
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The abstract explicitly contrasts mKikGR with the previously reported KikGR and with photoswitchable proteins currently available at the time.
Source-backed strengths
monomeric version of KikGR; high photostability; high switching rates; supports localization precision better than 10 nanometers
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monomeric version of KikGR
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high photostability
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high switching rates
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supports localization precision better than 10 nanometers
Compared with mMORp
mKikGR and mMORp address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with optogenetic probes
mKikGR and optogenetic probes address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with organoid fusion
mKikGR and organoid fusion address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.