Source 1review2018Viruses
Toolkit/multi-photon intravital microscopy
multi-photon intravital microscopy
Also known as: intravital microscopy using multi-photon excitation of fluorophores, MP-IVM, multi-photon microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Recently, intravital microscopy using multi-photon excitation of fluorophores has been applied to observe virus dissemination and pathogenesis in real-time under physiological conditions in living organisms. In this review, I summarize the latest research on in vivo studies of viral infections using multi-photon intravital microscopy (MP-IVM).
Usefulness & Problems
Why this is useful
MP-IVM is an in vivo imaging method that uses multi-photon excitation of fluorophores to visualize viral dissemination, infected cells, and immune responses in living organisms in real time.; real-time in vivo imaging of viral dissemination; visualizing virus-infected cells in living organisms; observing immune responses to viral infection under physiological conditions
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MP-IVM is an in vivo imaging method that uses multi-photon excitation of fluorophores to visualize viral dissemination, infected cells, and immune responses in living organisms in real time.
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real-time in vivo imaging of viral dissemination
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visualizing virus-infected cells in living organisms
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observing immune responses to viral infection under physiological conditions
Problem solved
It addresses the limitation of relying only on in vitro imaging by allowing observation of viral infection and pathogenesis under physiological conditions in vivo.; extends viral imaging beyond in vitro culture systems to living organisms; enables direct visualization of critical steps during viral infection and pathogenesis in vivo
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It addresses the limitation of relying only on in vitro imaging by allowing observation of viral infection and pathogenesis under physiological conditions in vivo.
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extends viral imaging beyond in vitro culture systems to living organisms
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enables direct visualization of critical steps during viral infection and pathogenesis in vivo
Problem links
This imaging method could help observe dynamic physiology under more native in vivo conditions, which may improve understanding of where current models diverge from living systems. It is plausibly useful for validating model fidelity, especially for complex tissue behavior.
enables direct visualization of critical steps during viral infection and pathogenesis in vivo
LiteratureIt addresses the limitation of relying only on in vitro imaging by allowing observation of viral infection and pathogenesis under physiological conditions in vivo.
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It addresses the limitation of relying only on in vitro imaging by allowing observation of viral infection and pathogenesis under physiological conditions in vivo.
extends viral imaging beyond in vitro culture systems to living organisms
LiteratureIt addresses the limitation of relying only on in vitro imaging by allowing observation of viral infection and pathogenesis under physiological conditions in vivo.
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It addresses the limitation of relying only on in vitro imaging by allowing observation of viral infection and pathogenesis under physiological conditions in vivo.
Published Workflows
Objective: Study viral infection, dissemination, pathogenesis, and host immune responses in living organisms by real-time in vivo imaging, while extending interpretability through complementary perturbation and molecular analysis.
Why it works: The review frames MP-IVM as enabling real-time observation of infection processes under physiological conditions, with animal preparation and fluorescent labeling providing access and signal, and optogenetic or transcriptional additions extending the significance of the imaging results.
multi-photon excitation of fluorophoresfluorescent labeling of viral or host featuresdirect visualization of virus dissemination and immune responsesintravital microscopymicrosurgical animal preparationfluorescent labelingoptogenetic tool integrationtranscriptional analysis
Stages
- 1.Microsurgical animal preparation(library_build)
The abstract explicitly notes experimental challenges during microsurgical animal preparation, indicating this is a prerequisite stage for in vivo intravital imaging.
Selection: Preparation of living animals for intravital imaging access
- 2.Fluorescent labeling for intravital imaging(library_design)
Fluorescent labeling strategies are discussed because MP-IVM relies on multi-photon excitation of fluorophores and direct visualization of fluorescent virus particles or infected cells.
Selection: Establish fluorescently visible targets for intravital imaging
- 3.In vivo multi-photon intravital imaging(functional_characterization)
This is the core stage that enables observation of viral infection processes in living organisms.
Selection: Real-time observation of virus dissemination, pathogenesis, and immune responses under physiological conditions
- 4.Complementary optogenetic and transcriptional integration(secondary_characterization)
The review explicitly highlights combining MP-IVM with optogenetic tools and transcriptional analysis as a powerful way to extend the significance of imaging studies.
Selection: Extend the significance of in vivo imaging studies
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The review states that MP-IVM depends on microsurgical animal preparation and fluorescent labeling strategies for intravital imaging.; multi-photon excitation microscopy setup; living-animal intravital imaging preparation; fluorescent labeling of virus particles, infected cells, or host components
The abstract does not claim that MP-IVM alone provides molecular perturbation or transcriptional readout; those are described as complementary additions when combined with other tools.; requires microsurgical animal preparation; depends on fluorescent labeling strategies for intravital imaging
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Critical steps during viral infection and pathogenesis can be studied by direct visualization of fluorescent virus particles, virus-infected cells, and the immune response to viral infection.
Combining MP-IVM with optogenetic tools and transcriptional analysis is described as a powerful approach to extend the significance of in vivo imaging studies of viral pathogens.
Much knowledge about virus effects on cells has originated from in vitro imaging studies using cell lines and primary cells, whereas MP-IVM enables in vivo observation under physiological conditions.
Multi-photon intravital microscopy has been applied to observe virus dissemination and pathogenesis in real time under physiological conditions in living organisms.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug multi-photon-intravital-microscopy
Recently, intravital microscopy using multi-photon excitation of fluorophores has been applied to observe virus dissemination and pathogenesis in real-time under physiological conditions in living organisms. In this review, I summarize the latest research on in vivo studies of viral infections using multi-photon intravital microscopy (MP-IVM).
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capabilitysupports
Critical steps during viral infection and pathogenesis can be studied by direct visualization of fluorescent virus particles, virus-infected cells, and the immune response to viral infection.
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combination strategysupports
Combining MP-IVM with optogenetic tools and transcriptional analysis is described as a powerful approach to extend the significance of in vivo imaging studies of viral pathogens.
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comparisonsupports
Much knowledge about virus effects on cells has originated from in vitro imaging studies using cell lines and primary cells, whereas MP-IVM enables in vivo observation under physiological conditions.
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review summarysupports
Multi-photon intravital microscopy has been applied to observe virus dissemination and pathogenesis in real time under physiological conditions in living organisms.
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Comparisons
Source-stated alternatives
The abstract contrasts MP-IVM with in vitro imaging studies using cell lines and primary cells as the prior dominant approach.
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The abstract contrasts MP-IVM with in vitro imaging studies using cell lines and primary cells as the prior dominant approach.
Source-backed strengths
supports real-time observation under physiological conditions; can directly visualize fluorescent virus particles, infected cells, and immune responses
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supports real-time observation under physiological conditions
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can directly visualize fluorescent virus particles, infected cells, and immune responses
Compared with imaging
The abstract contrasts MP-IVM with in vitro imaging studies using cell lines and primary cells as the prior dominant approach.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports real-time observation under physiological conditions; can directly visualize fluorescent virus particles, infected cells, and immune responses.
Relative tradeoffs: requires microsurgical animal preparation; depends on fluorescent labeling strategies for intravital imaging.
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The abstract contrasts MP-IVM with in vitro imaging studies using cell lines and primary cells as the prior dominant approach.
Compared with imaging surveillance
The abstract contrasts MP-IVM with in vitro imaging studies using cell lines and primary cells as the prior dominant approach.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports real-time observation under physiological conditions; can directly visualize fluorescent virus particles, infected cells, and immune responses.
Relative tradeoffs: requires microsurgical animal preparation; depends on fluorescent labeling strategies for intravital imaging.
Source:
The abstract contrasts MP-IVM with in vitro imaging studies using cell lines and primary cells as the prior dominant approach.
Ranked Citations
- 1.