Source 1review2020Frontiers in Bioengineering and Biotechnology
Toolkit/multiplexed engineering
multiplexed engineering
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Multiplexed engineering refers here to the use of the CRISPR/Cas toolbox for simultaneous genome engineering tasks in microbial biotechnology. The cited review places this approach in the context of metabolic engineering and high-throughput screening for production of chemicals and natural compounds.
Usefulness & Problems
Why this is useful
This approach is useful because it extends CRISPR/Cas from single-locus manipulation to multiplexed engineering in microbial systems. The review specifically frames it as relevant to metabolic engineering and high-throughput screening applications.
Source:
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Problem solved
It helps address the need to engineer microbial genomes in a multiplexed manner for biotechnology workflows. The cited source specifically links this need to metabolic engineering for chemical and natural product production.
Source:
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Problem links
Need better screening or enrichment leverage
DerivedMultiplexed engineering here refers to the application of the CRISPR/Cas toolbox for simultaneous genome engineering tasks in microbial biotechnology. The cited review places this approach in the context of metabolic engineering for production of chemicals and natural compounds and links it to high-throughput screening.
Need conditional recombination or state switching
DerivedMultiplexed engineering here refers to the application of the CRISPR/Cas toolbox for simultaneous genome engineering tasks in microbial biotechnology. The cited review places this approach in the context of metabolic engineering for production of chemicals and natural compounds and links it to high-throughput screening.
Need controllable genome or transcript editing
DerivedMultiplexed engineering here refers to the application of the CRISPR/Cas toolbox for simultaneous genome engineering tasks in microbial biotechnology. The cited review places this approach in the context of metabolic engineering for production of chemicals and natural compounds and links it to high-throughput screening.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
crispr/cas-mediated genome editingcrispr/cas-mediated genome editingrecombinationrecombinationTechniques
Functional AssayFunctional AssayFunctional AssaySelection / EnrichmentSelection / EnrichmentSelection / EnrichmentTarget processes
editingrecombinationselectionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: builder
The available evidence identifies CRISPR/Cas systems as the enabling toolbox and microbial biotechnology as the implementation context. However, the source excerpts provided do not specify Cas proteins, guide RNA architectures, delivery methods, host strains, or construct design requirements.
The provided evidence is limited to review-level statements about application areas and does not report specific organisms, editing efficiencies, multiplexing depth, or benchmarked outcomes. No direct experimental validation details are supplied in the evidence set.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
Approval Evidence
1 source1 linked approval claimfirst-pass slug multiplexed-engineering
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering
Source:
application scope summarysupports
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
Comparisons
Source-backed strengths
A key strength supported by the source is its application scope: CRISPR/Cas can be used for multiplexed engineering in microbial biotechnology. The same review also associates the approach with high-throughput screening and metabolic engineering applications.
Compared with CRISPR/Cas9
multiplexed engineering and CRISPR/Cas9 address a similar problem space because they share editing, recombination, selection.
Shared frame: same top-level item type; shared target processes: editing, recombination, selection
Strengths here: looks easier to implement in practice.
Compared with epigenome editing
multiplexed engineering and epigenome editing address a similar problem space because they share editing, recombination, selection.
Shared frame: same top-level item type; shared target processes: editing, recombination, selection
Compared with noncoding RNA manipulation
multiplexed engineering and noncoding RNA manipulation address a similar problem space because they share editing, recombination, selection.
Shared frame: same top-level item type; shared target processes: editing, recombination, selection
Ranked Citations
- 1.