The engineered optogenetic on switch is a light-inducible multi-component system that converts a transient blue light input into permanent transgene expression. It has been applied to drive recombination-linked gene expression programs, including spatially patterned differentiation in culture and pro-angiogenic factor expression in vivo.
Home/Mechanisms/recombination
Mechanism Concept
recombination
A mechanism-level grouping derived from the current toolkit evidence. Current coverage includes 2 architectures and 0 components. Across those tools, the main enabled capabilities are recombination.
2 total items2 architectures0 components
Main enabled capabilities: recombination.
Architectures
2 of 2we developed a dCas9-miniRecTE editor that enhances large-fragment integration without introducing double-strand breaks in human cells and primary mouse neurons, achieving ∼20% kilobase-scale knock-in efficiency.