Source 1primary paper2015Proceedings of the National Academy of Sciences
Toolkit/Nano-lantern
Nano-lantern
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Nano-lantern is a bright bioluminescent protein construct platform for real-time multicolor live-cell imaging. In the cited work, a previously developed yellowish-green Nano-lantern was expanded to cyan and orange luminescent variants, enabling imaging of intracellular structures, gene expression, and Ca(2+) dynamics.
Usefulness & Problems
Why this is useful
Nano-lantern is useful as a luminescent imaging platform for live-cell measurements that benefit from high brightness and multiple emission colors. The cited study reports applications including visualization of submicron intracellular structures, rapid endosome and peroxisome dynamics at about 1-second temporal resolution, simultaneous monitoring of multiple gene expression or Ca(2+) signals in one cell, and long-term focal adhesion imaging without photobleaching or photodamage.
Source:
Nano-lantern is a bright luminescent protein probe platform used for real-time multicolor live-cell imaging. In this paper, the palette was expanded to cyan and orange variants in addition to a previously developed yellowish-green form.
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real-time multicolor luminescence imaging
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live imaging without excitation light
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simultaneous monitoring of multiple gene expression or Ca(2+) dynamics
Problem solved
The cited work positions Nano-lantern as addressing the low brightness and limited color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated artifacts that can constrain fluorescence-based live imaging and experiments combined with optogenetic tools.
Source:
It addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
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addresses low brightness of existing luminescent protein probes
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expands poor color variants of luminescent protein probes
Source:
avoids excitation-light-associated autofluorescence, phototoxicity, photobleaching, and unintended optogenetic activation
Problem links
addresses low brightness of existing luminescent protein probes
LiteratureIt addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
Source:
It addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
avoids excitation-light-associated autofluorescence, phototoxicity, photobleaching, and unintended optogenetic activation
LiteratureIt addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
Source:
It addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
expands poor color variants of luminescent protein probes
LiteratureIt addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
Source:
It addresses the low brightness and poor color diversity of prior luminescent protein probes. It also provides an imaging modality that avoids excitation-light-associated problems relevant to fluorescence and optogenetic experiments.
Published Workflows
Objective: Develop an expanded multicolor Nano-lantern palette with improved brightness for real-time luminescence imaging in live cells.
Why it works: The abstract states that color change and brightness enhancement were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein, and that the resulting brightness enabled multicolor live imaging applications.
bioluminescence resonance energy transfer from enhanced Renilla luciferase to a fluorescent proteinprotein probe developmentmulticolor luminescence imaginglive-cell imaging
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
The reported design uses bioluminescence resonance energy transfer from enhanced Renilla luciferase to a fluorescent protein, indicating that Nano-lantern is an engineered fusion construct. Use therefore depends on expression of the luminescent protein construct in cells for luminescence imaging, but the supplied evidence does not specify substrate formulation, vector architecture, or delivery method.
The supplied evidence is limited to one cited study and mainly describes applications and general design principles rather than quantitative benchmarking. Detailed constraints on substrate requirements, instrumentation, absolute sensitivity, and performance across diverse organisms or in vivo settings are not provided in the abstract-level evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
continuous imaging duration a few hours
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
temporal resolution 1 s
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
brightness relative to wild-type Renilla luciferase 20 fold
Approval Evidence
1 source7 linked approval claimsfirst-pass slug nano-lantern
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
Source:
applicationsupports
Multicolor Nano-lanterns were extended to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell
Source:
applicationsupports
Nano-lanterns enabled continuous imaging of focal adhesion dynamics for longer than a few hours without photobleaching or photodamage.
the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage
Source:
applicationsupports
Nano-lanterns enabled visualization of rapid endosome and peroxisome dynamics at around 1-second temporal resolution.
The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution
Source:
applicationsupports
The brightness of the cyan and orange Nano-lanterns enabled multicolor live imaging of intracellular submicron structures.
which allowed us to perform multicolor live imaging of intracellular submicron structures
Source:
engineering advancesupports
The paper reports development of bright cyan and orange Nano-lantern luminescent proteins extending the previous Nano-lantern palette.
Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern.
Source:
mechanismsupports
Color change and brightness enhancement of the new Nano-lantern variants were achieved by BRET from enhanced Renilla luciferase to a fluorescent protein.
The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein.
Source:
performancesupports
The cyan and orange Nano-lanterns were approximately 20 times brighter than wild-type Renilla luciferase.
The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase
Source:
Comparisons
Source-stated alternatives
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Source:
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Source-backed strengths
The platform was reported to produce bright cyan and orange luminescent proteins in addition to the earlier yellowish-green form. This brightness supported multicolor live imaging of intracellular submicron structures, approximately 1-second imaging of endosome and peroxisome dynamics, simultaneous readout of multiple gene expression or Ca(2+) dynamics in distinct compartments, and continuous focal adhesion imaging for longer than a few hours without photobleaching or photodamage.
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bright color variants were developed
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supports multicolor live imaging of intracellular submicron structures
Source:
enabled imaging at around 1-s temporal resolution
Source:
allowed continuous imaging for longer than a few hours without photobleaching or photodamage
Compared with imaging
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Shared frame: source-stated alternative in extracted literature
Strengths here: bright color variants were developed; supports multicolor live imaging of intracellular submicron structures; enabled imaging at around 1-s temporal resolution.
Relative tradeoffs: the abstract does not specify all implementation details or performance tradeoffs across all colors.
Source:
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Compared with imaging surveillance
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Shared frame: source-stated alternative in extracted literature
Strengths here: bright color variants were developed; supports multicolor live imaging of intracellular submicron structures; enabled imaging at around 1-s temporal resolution.
Relative tradeoffs: the abstract does not specify all implementation details or performance tradeoffs across all colors.
Source:
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Compared with live imaging
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Shared frame: source-stated alternative in extracted literature
Strengths here: bright color variants were developed; supports multicolor live imaging of intracellular submicron structures; enabled imaging at around 1-s temporal resolution.
Relative tradeoffs: the abstract does not specify all implementation details or performance tradeoffs across all colors.
Source:
The abstract contrasts Nano-lantern-based luminescence imaging with fluorescence live imaging and with existing luminescent protein probes such as luciferases. Fluorescence is described as established but limited by excitation-light-related artifacts.
Ranked Citations
- 1.