Source 1primary paper2022Frontiers in Cell and Developmental Biology
Toolkit/near-infrared non-opsin optogenetic tool for nuclear export
near-infrared non-opsin optogenetic tool for nuclear export
Also known as: NIR OT, non-opsin optogenetic tool, OT
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The near-infrared non-opsin optogenetic tool for nuclear export is a genetically encoded multi-component system that induces nuclear export of a protein of interest in response to near-infrared light. In darkness, its effect is reversed by nuclear import, so subcellular localization depends on the balance between light-driven export and NLS-mediated import.
Usefulness & Problems
Why this is useful
This tool enables optical control of protein localization using near-infrared light, providing a way to regulate nuclear export of a protein of interest. The cited study specifically positions NLS choice as a means to optimize performance in neurons, where nuclear transport properties can differ from non-neuronal cells.
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This engineered optogenetic tool drives nuclear export of a protein of interest in response to near-infrared light. In darkness, nuclear import reverses the tool action.
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light-induced nuclear export of a protein of interest
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comparative analysis of nuclear transport dynamics in neurons
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light-controlled gene expression optimization in neurons
Problem solved
It addresses the need for controllable nuclear export of proteins of interest with light, particularly in neuronal contexts where standard nuclear localization signals may be suboptimal. The evidence indicates that tuning the nuclear localization signal can improve performance of the near-infrared export system.
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It addresses the need for controllable nuclear export and improved light-controlled gene expression performance in neurons, where nuclear transport differs from non-neuronal cells.
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provides near-infrared light control over nuclear export
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enables tuning of tool performance in neurons by varying NLSs
Problem links
enables tuning of tool performance in neurons by varying NLSs
LiteratureIt addresses the need for controllable nuclear export and improved light-controlled gene expression performance in neurons, where nuclear transport differs from non-neuronal cells.
Source:
It addresses the need for controllable nuclear export and improved light-controlled gene expression performance in neurons, where nuclear transport differs from non-neuronal cells.
provides near-infrared light control over nuclear export
LiteratureIt addresses the need for controllable nuclear export and improved light-controlled gene expression performance in neurons, where nuclear transport differs from non-neuronal cells.
Source:
It addresses the need for controllable nuclear export and improved light-controlled gene expression performance in neurons, where nuclear transport differs from non-neuronal cells.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
dark-state reversal by nuclear importdark-state reversal by nuclear importlight-induced nuclear exportlight-induced nuclear exportTechniques
No technique tags yet.
Target processes
localizationInput: Light
Implementation Constraints
cell context: neuronscofactor dependency: cofactor requirement unknowncontrolled process: nuclear exportencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementlight wavelength region: near-infraredmodality: optogeneticoperating role: actuatoroperating role: regulatorswitch architecture: multi component
Use of the system requires near-infrared illumination and a protein-of-interest construct configured with nuclear localization signal context that influences the import-export balance. The cited evidence supports domain fusion and signal-sequence tuning, but does not provide specific construct architecture, cofactors, or delivery methods in the supplied text.
The available evidence is limited to a description of near-infrared-induced nuclear export and dark-state reversal by nuclear import, with no quantitative kinetics, dynamic range, or wavelength details provided here. Performance remains sensitive to NLS choice, and the supplied evidence does not establish broad validation beyond the cited optimization context in neurons.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug near-infrared-non-opsin-optogenetic-tool-for-nuclear-export
Here we developed a non-opsin optogenetic tool (OT) for the nuclear export of a protein of interest induced by near-infrared (NIR) light.
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design rulesupports
Performance of the near-infrared optogenetic nuclear export tool can be adjusted by varying the nuclear localization signal.
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mechanism of actionsupports
In darkness, nuclear import reverses the action of the near-infrared non-opsin optogenetic nuclear export tool.
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optimization outcomesupports
Using selected NLSs, the authors optimized the near-infrared optogenetic tool for light-controlled gene expression to achieve lower background and higher contrast in neurons.
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tool developmentsupports
The authors developed a non-opsin optogenetic tool that induces nuclear export of a protein of interest with near-infrared light.
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Comparisons
Source-stated alternatives
The source contrasts uncommon neuron-suitable NLSs with widely used KPNA2-binding NLSs such as Myc and SV40, which were found to be suboptimal in neurons.
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The source contrasts uncommon neuron-suitable NLSs with widely used KPNA2-binding NLSs such as Myc and SV40, which were found to be suboptimal in neurons.
Source-backed strengths
The tool is described as a non-opsin optogenetic system that responds to near-infrared light to drive nuclear export. Its performance can be adjusted by varying the nuclear localization signal, providing a tunable design parameter for optimizing localization control in neurons.
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non-opsin design
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near-infrared light responsiveness
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performance can be adjusted by varying NLSs
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optimized for lower background and higher contrast in neurons
Compared with ArrayG
near-infrared non-opsin optogenetic tool for nuclear export and ArrayG address a similar problem space because they share localization.
Shared frame: same top-level item type; shared target processes: localization; same primary input modality: light
near-infrared non-opsin optogenetic tool for nuclear export and light-inducible nuclear translocation and dimerization system address a similar problem space because they share localization.
Shared frame: same top-level item type; shared target processes: localization; same primary input modality: light
near-infrared non-opsin optogenetic tool for nuclear export and optogenetic epigenetic editing toolbox for Ascl1 promoter targeting address a similar problem space because they share localization.
Shared frame: same top-level item type; shared target processes: localization; same primary input modality: light
Ranked Citations
- 1.