Source 1primary paper2019Communications Biology
Toolkit/Neuroligin-1 sfCherry3 Linker Across Synaptic Partners
Neuroligin-1 sfCherry3 Linker Across Synaptic Partners
Also known as: NLG-1 CLASP
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP) is a red trans-synaptic marker built from split sfCherry3 and neuroligin-1-based linkage across synaptic partners. It was reported to enable multiplexed visualization of neuronal synapses in living Caenorhabditis elegans.
Usefulness & Problems
Why this is useful
NLG-1 CLASP is useful for visualizing synaptic contacts in vivo with a red fluorescent channel, which supports multiplexed imaging alongside other markers. The associated split sfCherry3 system was also reported to facilitate endogenous protein tagging by gene editing, indicating compatibility with native-context labeling workflows.
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for multiplexed visualization of neuronal synapses in living C. elegans
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facilitating the tagging of endogenous proteins by gene editing
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Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Problem solved
This construct addresses the need for a red-colored trans-synaptic marker for live synapse visualization in C. elegans. It also contributes to the broader problem of expanding split fluorescent protein tools for multiplexed imaging and endogenous labeling.
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for multiplexed visualization of neuronal synapses in living C. elegans
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facilitating the tagging of endogenous proteins by gene editing
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Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuatorswitch architecture: split
The construct is described as a neuroligin-1-based trans-synaptic marker using split sfCherry3, implying a domain-fusion design across synaptic partners. The evidence supports use in living C. elegans, but the supplied material does not specify construct architecture, expression strategy, linker composition, or any required cofactors.
The supplied evidence does not provide quantitative performance data such as brightness, complementation efficiency, synapse specificity, or background signal for NLG-1 CLASP. Validation is only explicitly described for living C. elegans, and independent replication is not provided in the supplied record.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Approval Evidence
1 source2 linked approval claimsfirst-pass slug neuroligin-1-sfcherry3-linker-across-synaptic-partners
a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
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application demosupports
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
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tool developmentsupports
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
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Comparisons
Source-backed strengths
The reported strength of NLG-1 CLASP is its use as a red trans-synaptic marker that enabled multiplexed visualization of neuronal synapses in living C. elegans. The underlying split sfCherry3 platform was described in a study that improved split red fluorescent proteins using directed evolution and SpyTag/SpyCatcher-assisted strategies.
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The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Compared with joining proteins in creative ways
Neuroligin-1 sfCherry3 Linker Across Synaptic Partners and joining proteins in creative ways address a similar problem space.
Shared frame: same top-level item type
Compared with Nano-lantern
Neuroligin-1 sfCherry3 Linker Across Synaptic Partners and Nano-lantern address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with tethered PEs
Neuroligin-1 sfCherry3 Linker Across Synaptic Partners and tethered PEs address a similar problem space.
Shared frame: same top-level item type
Ranked Citations
- 1.