Source 1primary paper2013Frontiers in Molecular Neuroscience
Toolkit/neurotransmitter receptors modified with tethered photo-switchable ligands
neurotransmitter receptors modified with tethered photo-switchable ligands
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
There exists now a wide range of experimentally validated protein tools capable of modifying cellular function, including ... neurotransmitter receptors modified with tethered photo-switchable ligands.
Usefulness & Problems
Why this is useful
This tool class modifies neurotransmitter receptors with tethered photo-switchable ligands so cellular function can be controlled optically.; modifying cellular function; examining and manipulating neuronal function in vivo
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This tool class modifies neurotransmitter receptors with tethered photo-switchable ligands so cellular function can be controlled optically.
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modifying cellular function
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examining and manipulating neuronal function in vivo
Problem solved
It provides an optically addressable way to manipulate receptor-linked cellular function.; enable light-based control of receptor function
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It provides an optically addressable way to manipulate receptor-linked cellular function.
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enable light-based control of receptor function
Problem links
enable light-based control of receptor function
LiteratureIt provides an optically addressable way to manipulate receptor-linked cellular function.
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It provides an optically addressable way to manipulate receptor-linked cellular function.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Use requires the modified receptor-ligand system and light delivery.; requires receptor modification with tethered photo-switchable ligands; requires optical activation
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Combined optophysiology tools and methods have strong potential for studying neural circuits and networks, behavior, animal models of disease, and high-throughput ex vivo systems.
Optogenetics and genetically encoded photosensors have provided neuroscience researchers with many tools and methods for examining and manipulating neuronal function in vivo.
Experimentally validated protein tools for modifying cellular function include light-gated ion channels, recombinant light-gated G protein-coupled receptors, and neurotransmitter receptors modified with tethered photo-switchable ligands.
Genetically encoded protein sensors have been developed to optically track cellular activity in real time, including membrane-voltage-sensitive fluorophores and fluorescent calcium and pH indicators.
Approval Evidence
1 source1 linked approval claimfirst-pass slug neurotransmitter-receptors-modified-with-tethered-photo-switchable-ligands
There exists now a wide range of experimentally validated protein tools capable of modifying cellular function, including ... neurotransmitter receptors modified with tethered photo-switchable ligands.
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tool class capabilitysupports
Experimentally validated protein tools for modifying cellular function include light-gated ion channels, recombinant light-gated G protein-coupled receptors, and neurotransmitter receptors modified with tethered photo-switchable ligands.
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Comparisons
Source-stated alternatives
The abstract contrasts this class with light-gated ion channels and recombinant light-gated G protein-coupled receptors.
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The abstract contrasts this class with light-gated ion channels and recombinant light-gated G protein-coupled receptors.
Source-backed strengths
experimentally validated protein tool class
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experimentally validated protein tool class
Compared with recombinant light-gated G protein-coupled receptors
The abstract contrasts this class with light-gated ion channels and recombinant light-gated G protein-coupled receptors.
Shared frame: source-stated alternative in extracted literature
Strengths here: experimentally validated protein tool class.
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The abstract contrasts this class with light-gated ion channels and recombinant light-gated G protein-coupled receptors.
Ranked Citations
- 1.