Source 1primary paper2013Frontiers in Molecular Neuroscience
Toolkit/recombinant light-gated G protein-coupled receptors
recombinant light-gated G protein-coupled receptors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
There exists now a wide range of experimentally validated protein tools capable of modifying cellular function, including ... recombinant light-gated G protein-coupled receptors.
Usefulness & Problems
Why this is useful
These are recombinant light-gated G protein-coupled receptors used as protein tools to modify cellular function. The abstract places them among optophysiology actuators for neuroscience.; modifying cellular function; examining and manipulating neuronal function in vivo
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These are recombinant light-gated G protein-coupled receptors used as protein tools to modify cellular function. The abstract places them among optophysiology actuators for neuroscience.
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modifying cellular function
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examining and manipulating neuronal function in vivo
Problem solved
They provide a way to manipulate cellular function with light.; provide light-responsive receptor-based control of cellular function
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They provide a way to manipulate cellular function with light.
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provide light-responsive receptor-based control of cellular function
Problem links
provide light-responsive receptor-based control of cellular function
LiteratureThey provide a way to manipulate cellular function with light.
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They provide a way to manipulate cellular function with light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Use requires expression of the recombinant receptor and optical hardware for activation.; requires recombinant expression; requires optical activation
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Combined optophysiology tools and methods have strong potential for studying neural circuits and networks, behavior, animal models of disease, and high-throughput ex vivo systems.
Optogenetics and genetically encoded photosensors have provided neuroscience researchers with many tools and methods for examining and manipulating neuronal function in vivo.
Experimentally validated protein tools for modifying cellular function include light-gated ion channels, recombinant light-gated G protein-coupled receptors, and neurotransmitter receptors modified with tethered photo-switchable ligands.
Genetically encoded protein sensors have been developed to optically track cellular activity in real time, including membrane-voltage-sensitive fluorophores and fluorescent calcium and pH indicators.
Approval Evidence
1 source1 linked approval claimfirst-pass slug recombinant-light-gated-g-protein-coupled-receptors
There exists now a wide range of experimentally validated protein tools capable of modifying cellular function, including ... recombinant light-gated G protein-coupled receptors.
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tool class capabilitysupports
Experimentally validated protein tools for modifying cellular function include light-gated ion channels, recombinant light-gated G protein-coupled receptors, and neurotransmitter receptors modified with tethered photo-switchable ligands.
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Comparisons
Source-stated alternatives
The abstract lists light-gated ion channels and neurotransmitter receptors modified with tethered photo-switchable ligands as alternative actuator classes.
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The abstract lists light-gated ion channels and neurotransmitter receptors modified with tethered photo-switchable ligands as alternative actuator classes.
Source-backed strengths
experimentally validated protein tool class
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experimentally validated protein tool class
The abstract lists light-gated ion channels and neurotransmitter receptors modified with tethered photo-switchable ligands as alternative actuator classes.
Shared frame: source-stated alternative in extracted literature
Strengths here: experimentally validated protein tool class.
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The abstract lists light-gated ion channels and neurotransmitter receptors modified with tethered photo-switchable ligands as alternative actuator classes.
Ranked Citations
- 1.