Source 1review2013Reports on Progress in Physics
Toolkit/optical super-resolution microscopy
optical super-resolution microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
We will review some of the novel tools that have been developed for the observation of cellular activity and then discuss the recent breakthroughs in optical super-resolution microscopy that allow for optical observations beyond the diffraction limit.
Usefulness & Problems
Why this is useful
Optical super-resolution microscopy is presented as a set of optical observation methods that enable imaging beyond the diffraction limit. In this review it is framed as part of the toolkit for studying cellular activity at single-cell resolution.; observing cellular activity; optical observations beyond the diffraction limit; single-cell optical study in live organisms
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Optical super-resolution microscopy is presented as a set of optical observation methods that enable imaging beyond the diffraction limit. In this review it is framed as part of the toolkit for studying cellular activity at single-cell resolution.
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observing cellular activity
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optical observations beyond the diffraction limit
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single-cell optical study in live organisms
Problem solved
It addresses the need for higher-resolution optical observation of biological processes than conventional diffraction-limited microscopy allows.; improves spatial resolution of optical observation beyond the diffraction limit
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It addresses the need for higher-resolution optical observation of biological processes than conventional diffraction-limited microscopy allows.
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improves spatial resolution of optical observation beyond the diffraction limit
Problem links
improves spatial resolution of optical observation beyond the diffraction limit
LiteratureIt addresses the need for higher-resolution optical observation of biological processes than conventional diffraction-limited microscopy allows.
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It addresses the need for higher-resolution optical observation of biological processes than conventional diffraction-limited microscopy allows.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
No mechanism tags yet.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires optical microscopy instrumentation suited for super-resolution imaging in live biological samples. The abstract does not specify particular modalities or hardware details.; requires optical microscopy capability
The abstract does not state that it directly perturbs physiology or controls biomolecular activity; it is described as an observation technology.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optical super-resolution microscopy enables optical observations beyond the diffraction limit.
optical super-resolution microscopy that allow for optical observations beyond the diffraction limit
Photo-control of biomolecules allows local perturbations of physiological processes.
photo-control the activity of biomolecules, which allow for local perturbations of physiological processes
Approval Evidence
1 source1 linked approval claimfirst-pass slug optical-super-resolution-microscopy
We will review some of the novel tools that have been developed for the observation of cellular activity and then discuss the recent breakthroughs in optical super-resolution microscopy that allow for optical observations beyond the diffraction limit.
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capability summarysupports
Optical super-resolution microscopy enables optical observations beyond the diffraction limit.
optical super-resolution microscopy that allow for optical observations beyond the diffraction limit
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Comparisons
Source-stated alternatives
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
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The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Source-backed strengths
enables optical observations beyond the diffraction limit
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enables optical observations beyond the diffraction limit
Compared with imaging
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables optical observations beyond the diffraction limit.
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The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Compared with imaging surveillance
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables optical observations beyond the diffraction limit.
Source:
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Compared with optogenetic functional interrogation
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables optical observations beyond the diffraction limit.
Source:
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Compared with optogenetic membrane potential perturbation
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables optical observations beyond the diffraction limit.
Source:
The abstract contrasts observation tools with photo-control of biomolecules and optogenetics, which are presented as perturbation and monitoring approaches rather than purely super-resolution imaging.
Ranked Citations
- 1.