Source 1primary paper2017Nature Communications
Toolkit/optogenetic MAPK inhibitor
optogenetic MAPK inhibitor
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The optogenetic MAPK inhibitor is a reported construct design that uses light to inhibit MAPK signaling. In a 2017 Nature Communications report, this design was used to reveal resonance between transcription-regulating circuitry and temporally encoded inputs.
Usefulness & Problems
Why this is useful
This tool is useful for perturbing MAPK signaling with light as the input modality while probing how transcriptional circuitry responds to temporal patterns. The available evidence specifically supports its use for studying resonance between transcription-regulating circuitry and temporally encoded inputs.
Problem solved
It addresses the problem of controlling MAPK inhibition with an optogenetic input in order to interrogate temporally encoded signaling effects on transcription. The evidence does not provide further detail on the exact MAPK node, molecular architecture, or experimental context.
Problem links
Need precise spatiotemporal control with light input
DerivedThe optogenetic MAPK inhibitor is a reported construct design that uses light to inhibit MAPK signaling. In a 2017 Nature Communications report, this design was used to reveal resonance between transcription-regulating circuitry and temporally encoded inputs.
Need tighter control over gene expression timing or amplitude
DerivedThe optogenetic MAPK inhibitor is a reported construct design that uses light to inhibit MAPK signaling. In a 2017 Nature Communications report, this design was used to reveal resonance between transcription-regulating circuitry and temporally encoded inputs.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
optogenetic light-controlled inhibitionTechniques
Computational DesignTarget processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
Implementation is known only at the level that this is an optogenetic construct pattern using light to control MAPK inhibition. The evidence does not state construct composition, cofactors, delivery method, expression system, or assay configuration.
The supplied evidence does not specify the inhibitor components, light-responsive domain, wavelength, kinetics, dynamic range, or organismal system. Independent replication, comparative benchmarking, and breadth of validation are not established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
The paper reports a simple optogenetic MAPK inhibitor design.
The paper reports a simple optogenetic MAPK inhibitor design.
The paper reports a simple optogenetic MAPK inhibitor design.
The paper reports a simple optogenetic MAPK inhibitor design.
The paper reports a simple optogenetic MAPK inhibitor design.
The paper reports a simple optogenetic MAPK inhibitor design.
The paper reports a simple optogenetic MAPK inhibitor design.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug optogenetic-mapk-inhibitor
A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs
Source:
biological findingsupports
The reported optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally encoded inputs.
Source:
design reportsupports
The paper reports a simple optogenetic MAPK inhibitor design.
Source:
Comparisons
Source-backed strengths
The reported strength is conceptual simplicity, as the source describes it as a simple optogenetic MAPK inhibitor design. It was sufficiently functional in the reported study to reveal resonance between transcription-regulating circuitry and temporally encoded inputs.
Compared with 4pLRE-cPAOX1
optogenetic MAPK inhibitor and 4pLRE-cPAOX1 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Compared with blue-light-activated DNA template ON switch
optogenetic MAPK inhibitor and blue-light-activated DNA template ON switch address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Compared with VP16-EL222 light-responsive transcription factor
optogenetic MAPK inhibitor and VP16-EL222 light-responsive transcription factor address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Ranked Citations
- 1.