Source 1primary paper2026MED
Toolkit/OptoHEAL
OptoHEAL
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
OptoHEAL is described as a red-light-inducible version of the HEAL CRISPR activation platform for remote and precise transcriptional control. HEAL is a compact dCas12f-based CRISPRa architecture that activates transcription by recruiting transactivators through MS2 coat protein binding to MS2 aptamers embedded in the sgRNA scaffold.
Usefulness & Problems
Why this is useful
This tool is useful for programmable transcriptional activation in contexts where a compact CRISPRa architecture is advantageous. Its stated red-light inducibility suggests potential for remote temporal control, but the supplied evidence only directly supports the underlying HEAL recruitment architecture and comparative performance.
Source:
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
Source:
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
Source:
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
Source:
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
Problem solved
The underlying HEAL system addresses the need for a compact CRISPRa platform for programmable in vivo gene activation. OptoHEAL is described as extending this platform toward inducible control, but the supplied evidence does not detail how the red-light response is implemented.
Source:
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
Source:
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
Problem links
Need tighter control over gene expression timing or amplitude
DerivedOptoHEAL is described as a red-light-inducible version of the HEAL CRISPR activation platform for remote and precise transcriptional control. The underlying HEAL system is a compact dCas12f-based CRISPRa architecture that recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded in the sgRNA scaffold.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
crispra-mediated transcriptional activationcrispra-mediated transcriptional activationms2 aptamer-ms2 coat protein recruitmentms2 aptamer-ms2 coat protein recruitmentTechniques
No technique tags yet.
Target processes
transcriptionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: payload burdeninducible: Trueinduction modality: red lightoperating role: regulatorswitch architecture: multi componentswitch architecture: recruitment
The underlying HEAL architecture uses dCas12f together with sgRNAs containing embedded MS2 aptamers and transactivator recruitment via MS2 coat protein. No additional construct design, photoreceptor components, cofactors, delivery strategy, or expression-system details for OptoHEAL are provided in the supplied evidence.
The supplied evidence does not provide direct experimental details for OptoHEAL itself beyond its description as red-light inducible. No specific wavelengths, dynamic range, kinetics, target genes, organismal systems, or independent replication data are provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL outperforms existing CRISPRa systems in vitro and in vivo.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL recruits transactivators through MS2 coat protein binding to MS2 aptamers embedded within the sgRNA scaffold.
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
HEAL induces over 100,000-fold activation of endogenous genes.
gene activation fold change 100000
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
AAV-delivered HEAL targeting interleukin 10 alleviates acute kidney injury in mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
ChemHEAL-mediated activation of thymic stromal lymphopoietin reduces body weight in obese mice.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
HEAL is a high-efficiency dCas12f-based CRISPRa system for endogenous gene activation.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
ChemHEAL is a small-molecule-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
Approval Evidence
1 source1 linked approval claimfirst-pass slug optoheal
We further develop red-light-inducible OptoHEAL ... for remote and precise transcriptional control.
Source:
tool variantsupports
OptoHEAL is a red-light-inducible HEAL variant for remote and precise transcriptional control.
Source:
Comparisons
Source-backed strengths
According to the cited source, HEAL outperforms existing CRISPRa systems in vitro and in vivo. The platform is also described as compact and based on dCas12f, with transactivator recruitment mediated through engineered sgRNA-associated MS2 aptamers.
Source:
HEAL induces over 100,000-fold activation of endogenous genes.
Compared with opto-PKR
OptoHEAL and opto-PKR address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription
Strengths here: looks easier to implement in practice.
OptoHEAL and PmeR-OPmeR paraben-responsive mammalian transcription-control devices address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription
Compared with Set2-LANS optogenetic switch
OptoHEAL and Set2-LANS optogenetic switch address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription
Relative tradeoffs: appears more independently replicated.
Ranked Citations
- 1.