Source 1primary paper2020ACS Synthetic Biology
Toolkit/P_GAL1-S
P_GAL1-S
Also known as: engineered inducible GAL1 promoter, GAL1-S
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
P_GAL1-S is an engineered inducible GAL1 promoter for yeast reported to be stronger than constitutive or inducible promoters commonly used in this host. In the cited study, it was coupled to the OptoINVRT7 light-responsive circuit to drive strong, light-tunable gene expression and metabolic pathway control.
Usefulness & Problems
Why this is useful
P_GAL1-S is useful as a high-output yeast promoter for applications requiring strong inducible transcription. When paired with OptoINVRT7, it enabled light-tunable expression and supported improved production of lactic acid and isobutanol in metabolic engineering experiments.
Source:
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
Problem solved
This construct addresses the need for stronger inducible transcriptional output in yeast than is available from commonly used constitutive or inducible promoters. In the reported context, it also supported dynamic optical control of pathway gene expression for metabolic production tuning.
Problem links
Need precise spatiotemporal control with light input
DerivedP_GAL1-S is an engineered inducible GAL1 promoter for yeast reported to be stronger than constitutive or inducible promoters commonly used in this host. In the cited study, it was coupled to the OptoINVRT7 light-responsive circuit to drive strong, light-tunable gene expression and metabolic pathway control.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
The available evidence indicates use in yeast and functional coupling to the OptoINVRT7 light-responsive circuit. The supplied material does not specify promoter sequence changes, host strain, delivery format, or other construct design requirements.
The supplied evidence supports performance only in yeast and primarily in the context of coupling to the OptoINVRT7 circuit. Independent replication, cross-host validation, and construct-level design details for the promoter engineering are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
successYeastapplication demo
Inferred from claim c4 during normalization. Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness. Derived from claim c4. Quoted text: Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
fold induction in darkness132.9 foldfold induction variation22.6 fold
Supporting Sources
Ranked Claims
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
fold induction in darkness 132.9 foldfold induction variation 22.6 fold
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
The optogenetic circuit improved production of lactic acid by more than 50% and isobutanol by 15% when controlling metabolic enzymes.
The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50% and 15%, respectively.
isobutanol production increase 15 %lactic acid production increase 50 %
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 induces gene expression in 0.6 h after switching cells from light to darkness and is at least 6 times faster than previous OptoINVRT circuits used for chemical production.
The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chemical production.
induction time after light to dark switch 0.6 hspeed relative to previous OptoINVRT circuits 6 fold
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
OptoINVRT7 is a rapid optogenetic inverter circuit for controlling gene expression in Saccharomyces cerevisiae.
Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug p-gal1-s
In addition, we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
Source:
gene expression inductionsupports
Combining OptoINVRT7 with P_GAL1-S yields strong, light-tunable gene expression with up to 132.9 b1 22.6-fold induction in darkness.
Combining OptoINVRT7 with P_GAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 b1 22.6-fold induction in darkness.
Source:
promoter strengthsupports
P_GAL1-S is an engineered inducible GAL1 promoter reported to be stronger than constitutive or inducible promoters commonly used in yeast.
we introduce an engineered inducible GAL1 promoter (P_GAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast.
Source:
Comparisons
Source-backed strengths
The cited study reports that P_GAL1-S is stronger than any constitutive or inducible promoter commonly used in yeast. In combination with OptoINVRT7, it produced strong light-tunable expression with up to 132.9 ± 22.6-fold induction in darkness, and the associated optogenetic control scheme improved lactic acid production by more than 50% and isobutanol production by 15%.
Compared with mMORp
P_GAL1-S and mMORp address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with optogenetic probes
P_GAL1-S and optogenetic probes address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with organoid fusion
P_GAL1-S and organoid fusion address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.