Source 1primary paper2013Nature Communications
Toolkit/PAiRFP1
PAiRFP1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The paper reports bacteriophytochrome-derived photoactivatable near-infrared fluorescent proteins PAiRFP1 and PAiRFP2 that use biliverdin and undergo far-red-light-induced conversion to a brighter fluorescent state.
Usefulness & Problems
Why this is useful
PAiRFP1 is a bacteriophytochrome-derived photoactivatable near-infrared fluorescent protein. It converts to a brighter fluorescent state upon far-red light exposure.; photoactivatable near-infrared fluorescence imaging; far-red-light-controlled activation of fluorescent signal
Source:
PAiRFP1 is a bacteriophytochrome-derived photoactivatable near-infrared fluorescent protein. It converts to a brighter fluorescent state upon far-red light exposure.
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photoactivatable near-infrared fluorescence imaging
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far-red-light-controlled activation of fluorescent signal
Problem solved
It provides a genetically encoded photoactivatable fluorescent probe in the near-infrared range.; provides a photoactivatable near-infrared fluorescent protein derived from a bacterial phytochrome
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It provides a genetically encoded photoactivatable fluorescent probe in the near-infrared range.
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provides a photoactivatable near-infrared fluorescent protein derived from a bacterial phytochrome
Problem links
provides a photoactivatable near-infrared fluorescent protein derived from a bacterial phytochrome
LiteratureIt provides a genetically encoded photoactivatable fluorescent probe in the near-infrared range.
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It provides a genetically encoded photoactivatable fluorescent probe in the near-infrared range.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
biliverdin-dependent fluorescencefar-red-light-induced photoconversion to a brighter fluorescent statephotoactivationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: requires exogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
The reported construct uses biliverdin as its chromophore and is engineered from a bacterial phytochrome scaffold.; requires biliverdin chromophore
Requires extra cofactor or chromophore handling. Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. It may depend on an exogenous cofactor or chromophore supply step. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
PAiRFP1 and PAiRFP2 are photoactivatable near-infrared fluorescent proteins engineered from a bacterial phytochrome.
PAiRFP1 and PAiRFP2 undergo far-red-light-induced conversion to a brighter fluorescent state.
PAiRFP1 and PAiRFP2 use biliverdin as chromophore.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug pairfp1
The paper reports bacteriophytochrome-derived photoactivatable near-infrared fluorescent proteins PAiRFP1 and PAiRFP2 that use biliverdin and undergo far-red-light-induced conversion to a brighter fluorescent state.
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engineering resultsupports
PAiRFP1 and PAiRFP2 are photoactivatable near-infrared fluorescent proteins engineered from a bacterial phytochrome.
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mechanism or propertysupports
PAiRFP1 and PAiRFP2 undergo far-red-light-induced conversion to a brighter fluorescent state.
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mechanism or propertysupports
PAiRFP1 and PAiRFP2 use biliverdin as chromophore.
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Comparisons
Source-stated alternatives
The source-discovery summary identifies PAiRFP2 as a companion tool and iRFP and IFP1.4 as related predecessor bacteriophytochrome-derived fluorescent proteins.
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The source-discovery summary identifies PAiRFP2 as a companion tool and iRFP and IFP1.4 as related predecessor bacteriophytochrome-derived fluorescent proteins.
Source-backed strengths
uses biliverdin as chromophore; undergoes far-red-light-induced conversion to a brighter fluorescent state
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uses biliverdin as chromophore
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undergoes far-red-light-induced conversion to a brighter fluorescent state
Compared with PAiRFP2
The source-discovery summary identifies PAiRFP2 as a companion tool and iRFP and IFP1.4 as related predecessor bacteriophytochrome-derived fluorescent proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: uses biliverdin as chromophore; undergoes far-red-light-induced conversion to a brighter fluorescent state.
Source:
The source-discovery summary identifies PAiRFP2 as a companion tool and iRFP and IFP1.4 as related predecessor bacteriophytochrome-derived fluorescent proteins.
Ranked Citations
- 1.