Source 1primary paper2024Nucleic Acids Research
Toolkit/pcVP16
pcVP16
Also known as: pcVP16 transactivator, photo-controlling the VP16 (pcVP16) transactivation peptide
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
pcVP16 is a highly compact optogenetic regulator built by photo-controlling the VP16 transactivation peptide. It is described as a light-responsive construct for regulating transcriptional activation.
Usefulness & Problems
Why this is useful
pcVP16 is useful as a compact light-responsive transactivation module for optogenetic control of gene expression. The supplied evidence supports its design concept, but does not provide direct performance data for pcVP16 itself.
Source:
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
Source:
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Problem solved
pcVP16 addresses the need for compact optogenetic regulators that can place transcriptional activation under light control. The available evidence indicates that this is achieved by photo-controlling the VP16 transactivation peptide, but the specific implementation details are not provided.
Source:
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
Problem links
Need conditional recombination or state switching
DerivedpcVP16 is a highly compact optogenetic regulator built by photo-controlling the VP16 transactivation peptide. It is described as a light-responsive construct for regulating transcriptional activation.
Need precise spatiotemporal control with light input
DerivedpcVP16 is a highly compact optogenetic regulator built by photo-controlling the VP16 transactivation peptide. It is described as a light-responsive construct for regulating transcriptional activation.
Need tighter control over gene expression timing or amplitude
DerivedpcVP16 is a highly compact optogenetic regulator built by photo-controlling the VP16 transactivation peptide. It is described as a light-responsive construct for regulating transcriptional activation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
recombinationtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
pcVP16 is based on the VP16 transactivation peptide and was engineered by photo-controlling that peptide. The provided evidence does not specify the photosensory domain, construct architecture, cofactors, host system, or delivery method.
The supplied evidence does not report quantitative dynamic range, kinetics, wavelength dependence beyond general light responsiveness, or validation contexts specifically for pcVP16. Claims about blue-light transcriptional control and compatibility with multiple effectors are supported for LOOMINA in the same paper, but are not directly attributed to pcVP16 in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
LOOMINA combined with dCas9 controls transcription with blue light from endogenous promoters in multiple cell lines and shows exceptionally high dynamic ranges.
Leveraging the flexibility of CRISPR systems, we combined LOOMINA with dCas9 to control transcription with blue light from endogenous promoters with exceptionally high dynamic ranges in multiple cell lines.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
LOOMINA is a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
Then, applying a two-hybrid strategy, we engineered LOOMINA (light off-operated modular inductor of transcriptional activation), a versatile transcriptional control platform for mammalian cells that is compatible with various effector proteins.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug pcvp16
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
Source:
capabilitysupports
The authors created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Here, we inverted this mode of action and created optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Source:
designsupports
pcVP16 is a highly compact regulator created by photo-controlling the VP16 transactivation peptide.
First, we designed highly compact regulators by photo-controlling the VP16 (pcVP16) transactivation peptide.
Source:
significancesupports
LOOMINA and pcVP16 are valuable additions to the optogenetic repertoire for transcriptional regulation.
Functionally and mechanistically, the versatile LOOMINA platform and the exceptionally compact pcVP16 transactivator represent valuable additions to the optogenetic repertoire for transcriptional regulation.
Source:
Comparisons
Source-backed strengths
The main supported strength is compactness, as the source explicitly describes pcVP16 as a highly compact regulator. It is also explicitly framed as an optogenetic construct for controlling transcriptional activation with light.
Compared with 4pLRE-cPAOX1
pcVP16 and 4pLRE-cPAOX1 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
pcVP16 and phase-separation-engineered optogenetic synthetic transcription factors address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; same primary input modality: light
pcVP16 and photo-controlled VP16 transactivation peptide exposure regulators address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; same primary input modality: light
Ranked Citations
- 1.