Source 1primary paper2023
Toolkit/photo-controlled VP16 transactivation peptide exposure regulators
photo-controlled VP16 transactivation peptide exposure regulators
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photo-controlled VP16 transactivation peptide exposure regulators are highly compact optogenetic constructs that control exposure of the VP16 transactivation peptide with blue light. In the reported system, they were used as part of complementary platforms for blue-light-triggered termination of transcriptional activation and were incorporated into the LOOMINA toolbox.
Usefulness & Problems
Why this is useful
These regulators provide an optogenetic route to deactivate transcriptional activation with blue light rather than induce it. The reported integration of LOOMINA with Cas9 as a DNA-binding domain indicates utility for controlling transcription from various endogenous promoters.
Source:
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Problem solved
The tool addresses the need for compact optogenetic systems that terminate ongoing transcriptional activation in response to blue light. It specifically tackles how to make VP16-based activation conditional on light-controlled peptide exposure within a modular transcription-control framework.
Problem links
Need conditional recombination or state switching
DerivedPhoto-controlled VP16 transactivation peptide exposure regulators are highly compact optogenetic constructs that control exposure of the VP16 transactivation peptide with blue light. In the reported system, they were used as part of complementary platforms for blue-light-triggered termination of transcriptional activation and were incorporated into the LOOMINA toolbox.
Need precise spatiotemporal control with light input
DerivedPhoto-controlled VP16 transactivation peptide exposure regulators are highly compact optogenetic constructs that control exposure of the VP16 transactivation peptide with blue light. In the reported system, they were used as part of complementary platforms for blue-light-triggered termination of transcriptional activation and were incorporated into the LOOMINA toolbox.
Need tighter control over gene expression timing or amplitude
DerivedPhoto-controlled VP16 transactivation peptide exposure regulators are highly compact optogenetic constructs that control exposure of the VP16 transactivation peptide with blue light. In the reported system, they were used as part of complementary platforms for blue-light-triggered termination of transcriptional activation and were incorporated into the LOOMINA toolbox.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
recombinationtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The available evidence supports that the design centers on photo-controlling exposure of the VP16 transactivation peptide and that the broader LOOMINA system can be combined with Cas9 as a DNA-binding domain. The supplied text does not state the exact construct architecture, chromophore requirements, expression context, or delivery method.
The supplied evidence does not specify the photosensory domain, dynamic range, kinetics, leakiness, or quantitative performance of the VP16 exposure regulators. It also does not provide independent replication, organism-specific validation details, or direct evidence for recombination control by this specific construct pattern.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA was integrated with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters.
Leveraging the flexibility of CRISPR systems, we integrated LOOMINA with Cas9 as a DNA-binding domain to control transcription from various endogenous promoters
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA is a valuable addition to the optogenetic repertoire for transcriptional regulation based on functional and mechanistic results.
Both functionally and mechanistically, LOOMINA represents a valuable addition to the optogenetic repertoire for transcriptional regulation.
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA-Cas9 controlled transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells.
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
dynamic range exceptionally high
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
LOOMINA is a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins.
we engineered LOOMINA ... a versatile transcriptional control platform for mammalian cells that is highly adaptable and compatible with various effector proteins
Approval Evidence
1 source2 linked approval claimsfirst-pass slug photo-controlled-vp16-transactivation-peptide-exposure-regulators
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Source:
capabilitysupports
The paper reports two complementary optogenetic systems that terminate transcriptional activation in response to blue light.
Here, we inverted this mode of action and created two complementary optogenetic systems capable of efficiently terminating transcriptional activation in response to blue light.
Source:
engineering strategysupports
Highly compact regulators were designed by photo-controlling VP16 transactivation peptide exposure.
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Source:
Comparisons
Source-backed strengths
The source describes these regulators as highly compact, which is a practical design advantage for construct engineering. They were reported within a system that enables blue-light-responsive termination of transcriptional activation, and the broader toolbox was integrated with Cas9 to target various endogenous promoters.
Source:
First, we designed highly compact regulators, by photo-controlling VP16 transactivation peptide exposure.
Source:
to control transcription from various endogenous promoters with exceptionally high dynamic ranges in multiple cell lines, including neuron-like cells
Compared with 4pLRE-cPAOX1
photo-controlled VP16 transactivation peptide exposure regulators and 4pLRE-cPAOX1 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Compared with pcVP16
photo-controlled VP16 transactivation peptide exposure regulators and pcVP16 address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; same primary input modality: light
photo-controlled VP16 transactivation peptide exposure regulators and phase-separation-engineered optogenetic synthetic transcription factors address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; same primary input modality: light
Ranked Citations
- 1.