Source 1primary paper2020
Toolkit/photo-caged mRNA
photo-caged mRNA
Also known as: photo-cages, photo-cages tethered to the 5′ untranslated region (5′-UTR) of an mRNA
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photo-caged mRNA is an mRNA engineering strategy in which small-molecule caging groups are tethered to the 5′ untranslated region to suppress translation until illumination. Photocleavage of the cages activates translation and enables single-cell spatiotemporal control in mammalian cells.
Usefulness & Problems
Why this is useful
This tool provides optical control over mRNA translation with single-cell spatial and temporal precision in mammalian cells. The cited study also shows that two distinct mRNAs can be sequentially activated in the same cell, supporting multiplexed control of gene expression.
Source:
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
Source:
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Problem solved
Photo-caged mRNA addresses the problem of how to keep exogenous mRNA translationally silent until a defined time and place. It specifically solves the need for light-triggered, localized activation of translation from mRNA constructs in mammalian cells.
Problem links
Need precise spatiotemporal control with light input
DerivedPhoto-caged mRNA consists of small-molecule caging groups tethered to the 5′ untranslated region of an mRNA to suppress translation until light exposure. Photocleavage of the cages releases this repression and activates translation with single-cell spatiotemporal resolution in mammalian cells.
Need tighter control over protein production
DerivedPhoto-caged mRNA consists of small-molecule caging groups tethered to the 5′ untranslated region of an mRNA to suppress translation until light exposure. Photocleavage of the cages releases this repression and activates translation with single-cell spatiotemporal resolution in mammalian cells.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
PhotocleavagePhotocleavagePhotocleavagetranslation controltranslation controlTranslation ControlTechniques
No technique tags yet.
Target processes
translationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: cleavage
The defining construct feature is tethering small-molecule photo-cages to the 5′ untranslated region of an mRNA. The available evidence supports use in mammalian cells and sequential optical activation of two mRNA species, but it does not specify cage chemistries, illumination wavelengths, delivery methods, or expression workflows.
The supplied evidence is limited to a single 2020 study and does not provide quantitative performance metrics such as fold repression, activation kinetics, wavelength ranges, or phototoxicity. Evidence is also limited to mammalian cells, with no support here for in vivo use, broad organismal portability, or long-term expression studies.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug photo-caged-mrna
small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA
Source:
mechanistic effectsupports
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Source:
mechanistic effectsupports
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Source:
method capabilitysupports
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Source:
Comparisons
Source-backed strengths
Conjugation of photo-cages to the 5′-UTR severely reduces translation, indicating strong repression before illumination. Photo-release of the cages triggers translation activation with single-cell spatiotemporal resolution, and sequential optical activation of two mRNAs in the same mammalian cell was reported.
Compared with tet-controlled riboregulatory module
photo-caged mRNA and tet-controlled riboregulatory module address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation control, translation_control; same primary input modality: light
Compared with upstream ORFs
photo-caged mRNA and upstream ORFs address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Compared with wavelength-selective photo-cage pair for mRNA
photo-caged mRNA and wavelength-selective photo-cage pair for mRNA address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: photocleavage, translation control, translation_control; same primary input modality: light
Ranked Citations
- 1.