Source 1primary paper2020
Toolkit/wavelength-selective photo-cage pair for mRNA
wavelength-selective photo-cage pair for mRNA
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is a pair of wavelength-selective photo-cages conjugated to the 5′-UTR of mRNA to suppress translation until illumination. Selective photocleavage with different wavelengths enables sequential optical activation of two distinct mRNAs in the same mammalian cell with single-cell spatiotemporal resolution.
Usefulness & Problems
Why this is useful
The system provides optical control over mRNA translation with both spatial and temporal precision at the single-cell level. Its key utility is multiplexed regulation, because two different mRNAs can be activated sequentially by using photo-cages that respond to different wavelengths of light.
Source:
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
Source:
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Problem solved
It addresses the problem of independently controlling translation of multiple mRNA species in the same mammalian cell using light. The approach specifically solves how to keep mRNAs translationally repressed until user-defined photo-irradiation and then release them in sequence.
Problem links
Need precise spatiotemporal control with light input
DerivedThis tool is a pair of wavelength-selective photo-cages conjugated to the 5′-UTR of mRNA to suppress translation until illumination. Selective photocleavage with different wavelengths enables sequential optical activation of two distinct mRNAs in the same mammalian cell with single-cell spatiotemporal resolution.
Need tighter control over protein production
DerivedThis tool is a pair of wavelength-selective photo-cages conjugated to the 5′-UTR of mRNA to suppress translation until illumination. Selective photocleavage with different wavelengths enables sequential optical activation of two distinct mRNAs in the same mammalian cell with single-cell spatiotemporal resolution.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
translationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: cleavage
The photo-cages are tethered to the 5′-UTR of mRNA, and translation is activated by photo-release after irradiation with different wavelengths of light. The available evidence supports use in mammalian cells, but it does not specify delivery format, expression workflow, or detailed conjugation chemistry beyond photo-cage attachment to mRNA.
The supplied evidence is limited to a single 2020 source and focuses on translation suppression and sequential activation in mammalian cells. Specific cage chemistries, exact activation wavelengths, quantitative dynamic range, and validation across broader cell types or in vivo settings are not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Conjugation of photo-cages onto the 5′-UTR severely reduces mRNA translation.
Translation of mRNA was severely reduced upon conjugation of the ‘photo-cages’ onto the 5′-UTR.
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
Photo-release of cages from mRNA triggers activation of translation with single-cell spatiotemporal resolution.
subsequent photo-release of the ‘cages’ from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug wavelength-selective-photo-cage-pair-for-mrna
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light
Source:
method capabilitysupports
A pair of photo-cages can be selectively cleaved from mRNA using different wavelengths of light to enable sequential photo-activation of two mRNAs.
we synthesized a pair of ‘photo-cages’ which can be selectively cleaved from mRNA upon photo-irradiation with different wavelengths of light. Sequential photo-activation of two mRNAs enabled precise optical control of translation of two unique transcripts.
Source:
method capabilitysupports
Two types of mRNAs can be sequentially optically activated in the same mammalian cell by sequential photocleavage of photo-cages tethered to the 5′-UTR.
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups (‘photo-cages’) tethered to the 5′ untranslated region (5′-UTR) of an mRNA.
Source:
Comparisons
Source-backed strengths
Evidence indicates that 5′-UTR conjugation of photo-cages severely reduces mRNA translation, providing an effective OFF state. The paired cages are wavelength-selective and can be photocleaved sequentially, enabling activation of two mRNAs in the same mammalian cell with single-cell spatiotemporal resolution.
Compared with microRNA
wavelength-selective photo-cage pair for mRNA and microRNA address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Compared with PAL
wavelength-selective photo-cage pair for mRNA and PAL address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Compared with photo-caged mRNA
wavelength-selective photo-cage pair for mRNA and photo-caged mRNA address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: photocleavage, translation control, translation_control; same primary input modality: light
Ranked Citations
- 1.