Source 1primary paper2020Molecules
Toolkit/photoactivatable cyclic caged morpholino oligomers
photoactivatable cyclic caged morpholino oligomers
Also known as: caged antisense reagents, ccMOs
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photoactivatable cyclic caged morpholino oligomers (ccMOs) are light-responsive antisense morpholino reagents engineered in a cyclic, caged format to suppress target binding until photoactivation. In the reported design, brief 405-nm illumination photocleaves the cage and restores antisense activity, enabling spatiotemporal regulation of gene expression.
Usefulness & Problems
Why this is useful
ccMOs are useful for modulating gene function with spatial and temporal precision in vivo. The light-gated design allows antisense activity to remain suppressed until illumination, supporting controlled perturbation of gene expression.
Source:
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
Source:
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Problem solved
These reagents address the problem of achieving precise temporal and spatial control over morpholino-mediated gene regulation. The cyclic caging strategy specifically solves premature target binding by inhibiting DNA binding until light exposure restores activity.
Source:
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
Problem links
Need precise spatiotemporal control with light input
DerivedPhotoactivatable cyclic caged morpholino oligomers (ccMOs) are light-responsive antisense morpholino reagents engineered in a cyclic, caged format to suppress target binding until photoactivation. In the reported design, brief 405-nm illumination photocleaves the cage and restores antisense activity, enabling spatiotemporal regulation of gene expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
cyclization-based cagingDNA Bindingdna binding inhibitiondna binding inhibition and light-restored target bindinglight-restored target bindingOligomerizationPhotocleavageTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: cleavage
The reported activation wavelength is 405 nm, and the construct uses a cyclic morpholino architecture with a photocleavable linker. An ethynyl-functionalized photocleavable linker was introduced to facilitate preparation, with click chemistry coupling and chemical linker engineering indicated by the source metadata.
The supplied evidence supports light-triggered activity control and synthetic improvement, but provides limited quantitative performance data. Independent replication, breadth across targets or organisms, and detailed in vivo validation outcomes are not established from the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
activation wavelength 405 nm
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Introducing an ethynyl function on the photocleavable linker facilitates Huisgen 1,3-dipolar cycloaddition coupling with the oligonucleotide and reduces synthetic steps while improving total yield and linker stability compared with previous strategies.
The key factor is the introduction of an ethynyl function on the photocleavable linker to facilitate the use of a Huisgen 1,3-dipolar cycloaddition for the coupling reaction with the oligonucleotide. Compared to previous strategies, this modification reduces the number of synthetic steps and significantly improves the total yield and the stability of the linker.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug photoactivatable-cyclic-caged-morpholino-oligomers
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Source:
activity controlsupports
The caging strategy inhibits DNA binding ability and activity can be restored by brief illumination with 405-nm light.
HPLC analysis confirms that the caging strategy successfully inhibits the DNA binding ability, and the activity can be restored by brief illumination with 405-nm light.
Source:
application potentialsupports
These caged antisense reagents are presented as excellent tools to modulate gene function in vivo with spatial and temporal precision.
Overall, the straightforward preparation together with the clean and fast photochemistry make these caged antisense reagents excellent tools to modulate gene function in-vivo with spatial and temporal precision.
Source:
design improvementsupports
The novel ccMO design with an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
We describe a novel ccMO design that overcomes many of the challenges and considerably expedites the synthetic preparation.
Source:
tool capabilitysupports
Photoactivatable cyclic caged morpholino oligomers can selectively regulate gene expression with spatiotemporal control.
Photoactivatable cyclic caged morpholino oligomers (ccMOs) represent a promising tool to selectively regulate gene expression with spatiotemporal control.
Source:
Comparisons
Source-backed strengths
The reported caging strategy inhibits DNA binding and antisense activity, and activity can be restored by brief 405-nm illumination. The 2020 Molecules report also states that an ethynyl-functionalized photocleavable linker expedites synthetic preparation and overcomes many preparation challenges.
Compared with phosphorothioate-caged antisense oligonucleotides
photoactivatable cyclic caged morpholino oligomers and phosphorothioate-caged antisense oligonucleotides address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Compared with photo-sensitive circular gRNAs
photoactivatable cyclic caged morpholino oligomers and photo-sensitive circular gRNAs address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
photoactivatable cyclic caged morpholino oligomers and small interfering RNA with randomly incorporated photolabile groups address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
- 1.