Source 1primary paper2005Angewandte Chemie International Edition
Toolkit/photolabile-modified small interfering RNA
photolabile-modified small interfering RNA
Also known as: light-activated siRNA, siRNA with photolabile moieties
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photolabile-modified small interfering RNA is a chemically caged siRNA reagent whose RNA interference activity is suppressed before illumination and restored by light exposure. Upon irradiation, the modified siRNA is released into an active state that suppresses target gene expression.
Usefulness & Problems
Why this is useful
This tool enables optical control of RNA interference, allowing cellular mRNA levels regulated by the RNA interference pathway to be controlled with light. It is useful when conditional activation of siRNA is needed rather than constitutive knockdown.
Problem solved
It addresses the problem of unwanted siRNA activity before the desired experimental time by chemically blocking RNA interference until illumination. The cited work specifically frames this as light-dependent control over target gene suppression through the RNA interference pathway.
Problem links
Need precise spatiotemporal control with light input
DerivedPhotolabile-modified small interfering RNA is a chemically caged siRNA reagent whose RNA interference activity is suppressed before illumination and restored by light exposure. Upon irradiation, the modified siRNA is released into an active state that suppresses target gene expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: cleavage
Implementation requires chemical modification of siRNA with photolabile moieties to cage its activity prior to illumination. The available evidence does not provide details on construct design, delivery method, cell type, or optical parameters beyond the requirement for irradiation.
The provided evidence does not specify the photolabile chemistry, illumination wavelength, uncaging efficiency, or quantitative knockdown performance. Validation breadth is limited here to a single cited study and a general description of light-activated RNA interference.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug photolabile-modified-small-interfering-rna
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification. Upon irradiation, siRNA is released.
Source:
activity blockingsupports
Photolabile modification of siRNA can partially or fully block RNA interference depending on the extent of modification.
The modification of small interfering RNA (siRNA) with photolabile moieties can partially or fully block RNA interference, depending on the extent of siRNA modification.
Source:
control modalitysupports
Cellular mRNA levels regulated by the RNA interference pathway can be controlled with light.
Cellular mRNA levels that are regulated by the RNA interference pathway can now be controlled with light.
Source:
light activationsupports
Upon irradiation, the modified siRNA is released into an active state that can suppress target gene expression.
Upon irradiation, siRNA is released (see picture). Now in its active state, it is able to suppress target gene expression.
Source:
Comparisons
Source-backed strengths
The source reports that photolabile modification can partially or fully block RNA interference, depending on the extent of siRNA modification. It also reports that irradiation releases the siRNA into an active state capable of suppressing target gene expression, demonstrating reversible control by light.
Compared with phosphorothioate-caged antisense oligonucleotides
photolabile-modified small interfering RNA and phosphorothioate-caged antisense oligonucleotides address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Compared with photo-sensitive circular gRNAs
photolabile-modified small interfering RNA and photo-sensitive circular gRNAs address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
photolabile-modified small interfering RNA and small interfering RNA with randomly incorporated photolabile groups address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
- 1.