Source 1review2017Chemical Reviews
Toolkit/Phytochrome-based reporters and biosensors
Phytochrome-based reporters and biosensors
Also known as: reporters and biosensors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Phytochrome-based reporters and biosensors are construct designs derived from phytochrome systems for near-infrared sensing applications. They have been described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications, particularly in contexts relevant to mammalian cells and in vivo use.
Usefulness & Problems
Why this is useful
These tools are useful as near-infrared probes for sensing biological events in settings where noninvasive imaging and light-based applications are desired. The cited review specifically positions them within probe-selection frameworks for mammalian-cell and in vivo applications.
Source:
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Problem solved
They address the need to detect molecular events such as protein-protein interactions, proteolysis, and posttranslational modification using phytochrome-derived reporter architectures. The available evidence does not provide more specific information about individual assay formats or performance benchmarks.
Source:
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Problem links
Need better screening or enrichment leverage
DerivedPhytochrome-based reporters and biosensors are construct designs derived from phytochrome systems for near-infrared sensing applications. They have been described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications, particularly in contexts relevant to mammalian cells and in vivo use.
Need precise spatiotemporal control with light input
DerivedPhytochrome-based reporters and biosensors are construct designs derived from phytochrome systems for near-infrared sensing applications. They have been described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications, particularly in contexts relevant to mammalian cells and in vivo use.
Need tighter control over protein production
DerivedPhytochrome-based reporters and biosensors are construct designs derived from phytochrome systems for near-infrared sensing applications. They have been described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications, particularly in contexts relevant to mammalian cells and in vivo use.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
Translation ControlTarget processes
selectiontranslationInput: Light
Implementation Constraints
cofactor dependency: requires exogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The evidence supports intended use in mammalian cells and in vivo applications and situates these constructs among near-infrared probes and light-manipulation tools. However, the supplied material does not specify chromophore requirements, domain architecture, delivery strategy, or expression-system details for implementation.
The supplied evidence is review-level and does not identify specific reporter constructs, dynamic range, sensitivity, kinetics, or validation datasets. It also does not establish whether any particular phytochrome-based biosensor was independently replicated beyond the reviewed literature.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
Phytochromes are attractive molecular templates for engineering light-sensing probes because of their multidomain structure and autocatalytic incorporation of linear tetrapyrrole chromophores.
Their multidomain structure and autocatalytic incorporation of linear tetrapyrrole chromophores make phytochromes attractive molecular templates for the development of light-sensing probes.
Bacterial phytochromes use biliverdin as a chromophore and exhibit strongly near-infrared-shifted spectra within the tissue transparency window.
A subclass of bacterial phytochromes (BphPs) utilizes heme-derived biliverdin tetrapyrrole, which is ubiquitous in mammalian tissues, as a chromophore. Because biliverdin possesses the largest electron-conjugated chromophore system among linear tetrapyrroles, BphPs exhibit the most NIR-shifted spectra that reside within the NIR tissue transparency window.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug phytochrome-based-reporters-and-biosensors
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Source:
application scopesupports
Phytochrome-based reporters and biosensors are described for detecting protein-protein interactions, proteolytic activities, and posttranslational modifications.
We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications.
Source:
intended use scopesupports
The review provides selection guidelines for near-infrared probes and tools intended for noninvasive imaging, sensing, and light-manipulation applications, with a focus on mammalian cells and in vivo use.
Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
Source:
Comparisons
Source-backed strengths
A key strength is their stated applicability to multiple biological readouts, including interaction detection, proteolytic activity, and posttranslational modification sensing. The review also places these tools in the near-infrared and in vivo imaging context, which supports relevance for mammalian systems.
Compared with 4pLRE-cPAOX1
Phytochrome-based reporters and biosensors and 4pLRE-cPAOX1 address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with blue-light-activated DNA template ON switch
Phytochrome-based reporters and biosensors and blue-light-activated DNA template ON switch address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with triple brake design
Phytochrome-based reporters and biosensors and triple brake design address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.