Source 1review2025MED
Toolkit/polymerase chain reactions
polymerase chain reactions
Also known as: PCR
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
While these methods, like polymerase chain reactions or whole-genome sequencing, are considered the "gold standard" for diagnostics, the development of inexpensive, rapid diagnostic assays is necessary for effective AMR detection and management.
Usefulness & Problems
Why this is useful
Polymerase chain reactions are described as gold-standard diagnostic methods for AMR detection. In this review context they serve as established comparators to emerging rapid assays.; gold-standard diagnostics for AMR genes
Source:
Polymerase chain reactions are described as gold-standard diagnostic methods for AMR detection. In this review context they serve as established comparators to emerging rapid assays.
Source:
gold-standard diagnostics for AMR genes
Problem solved
PCR supports molecular detection of antimicrobial resistance genes. The review uses it as a benchmark for current diagnostic practice.; established molecular detection of antimicrobial resistance genes
Source:
PCR supports molecular detection of antimicrobial resistance genes. The review uses it as a benchmark for current diagnostic practice.
Source:
established molecular detection of antimicrobial resistance genes
Problem links
PCR is directly relevant to pathogen detection and is a practical first-line surveillance assay for known targets. It could support rapid confirmation and monitoring after an emerging threat has been identified by sequencing or other discovery methods.
established molecular detection of antimicrobial resistance genes
LiteraturePCR supports molecular detection of antimicrobial resistance genes. The review uses it as a benchmark for current diagnostic practice.
Source:
PCR supports molecular detection of antimicrobial resistance genes. The review uses it as a benchmark for current diagnostic practice.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
No mechanism tags yet.
Target processes
diagnosticImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Operational role: sensor. Implementation mode: genetically encoded. Cofactor status: cofactor requirement unknown.
The abstract states that current methods such as PCR are expensive and time-consuming. It therefore does not meet the stated need for inexpensive, rapid testing in all settings.; expensive; time-consuming
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Biosensors have potential for diagnostic testing because they are easy to use, use inexpensive materials, provide rapid results, and are portable.
Combining biosensors with nanomaterials can produce sensitive and easily interpretable diagnostic results.
Current methods for detecting antimicrobial resistance genes are expensive and time-consuming.
Polymerase chain reactions and whole-genome sequencing are considered gold-standard diagnostics for antimicrobial resistance detection.
There is a need for inexpensive, rapid diagnostic assays for effective antimicrobial resistance detection and management.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug polymerase-chain-reactions
While these methods, like polymerase chain reactions or whole-genome sequencing, are considered the "gold standard" for diagnostics, the development of inexpensive, rapid diagnostic assays is necessary for effective AMR detection and management.
Source:
review summarysupports
Current methods for detecting antimicrobial resistance genes are expensive and time-consuming.
Source:
review summarysupports
Polymerase chain reactions and whole-genome sequencing are considered gold-standard diagnostics for antimicrobial resistance detection.
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Comparisons
Source-stated alternatives
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
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The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Source-backed strengths
considered a gold-standard diagnostic method
Source:
considered a gold-standard diagnostic method
Compared with biosensors
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Shared frame: source-stated alternative in extracted literature
Strengths here: considered a gold-standard diagnostic method.
Relative tradeoffs: expensive; time-consuming.
Source:
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Compared with biosensors for active Rho detection
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Shared frame: source-stated alternative in extracted literature
Strengths here: considered a gold-standard diagnostic method.
Relative tradeoffs: expensive; time-consuming.
Source:
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Compared with fluorescent protein based reporters and biosensors
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Shared frame: source-stated alternative in extracted literature
Strengths here: considered a gold-standard diagnostic method.
Relative tradeoffs: expensive; time-consuming.
Source:
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Compared with genetically engineered biosensors
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Shared frame: source-stated alternative in extracted literature
Strengths here: considered a gold-standard diagnostic method.
Relative tradeoffs: expensive; time-consuming.
Source:
The abstract contrasts PCR with biosensors and also mentions whole-genome sequencing as another gold-standard method.
Ranked Citations
- 1.